TCP1 delta (CCT4) (NM_006430) Human Tagged ORF Clone

SKU RC214480

CCT4 (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 4 (delta) (CCT4)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TCP1 delta
• Synonyms: CCT-DELTA; Cctd; SRB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214480 representing NM_006430; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGGAAAGGCGCCT; ATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCCGCCAAAGCGGTTGCTGATGC; TATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATGATTCAAGATGGAAAAGGTGATGTAACCATT; ACAAATGATGGTGCTACCATTCTGAAACAAATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGC; TGTCTAAGGCTCAAGATATAGAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTT; AGATTCTTGTACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC; CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGAGAAACTTTGT; TAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCAAGTCTGCTTTCTCCAATGAG; TGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCCACCAGTGTAGATCTTAGAGATATTAAAATA; GTTAAGAAGCTTGGTGGGACAATTGATGACTGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGT; CAAATTCTGGCATAACCAGAGTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAA; AACAGACATGGATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA; GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATACAGAAATCTA; TTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATGAAGATCATGGTGATTAAGGA; TATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATTGGAACCAAGCCAGTTGCTCATATTGACCAA; TTTACTGCTGACATGCTGGGTTCTGCTGAGTTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGC; TCAAGATTACAGGCTGTGCCAGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGT; GATTGAAGAAGCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT; CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCACGAACACTGA; GTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTCATTCCATCTACACTAGCTGA; AAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTAAGAAACCGGCATGCCCAGGGAGAAAAAACT; GCAGGCATTAATGTCCGAAAGGGTGGTATTTCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGG; TATCAGTCAGTGCTCTGACTCTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAA; CACTCGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214480 representing NM_006430; Red=Cloning site Green=Tags(s); ; MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTI; TNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKA; LEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKI; VKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREER; AYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQ; FTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRA; LIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKT; AGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006430
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006430.4
• RefSeq Size: 2349 bp
• RefSeq ORF: 1620 bp
• Locus ID: 10575
• UniProt ID: P50991
• Cytogenetics: 2p15
• Domains: cpn60_TCP1
• Protein Families: Druggable Genome
• MW: 57.7 kDa
• Summary: The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008]