SAMD9L (NM_152703) Human Tagged ORF Clone

  • TrueORF®
SKU
RC214427
SAMD9L (Myc-DDK-tagged)-Human sterile alpha motif domain containing 9-like (SAMD9L)
$589.00 $2,032.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SAMD9L
Synonyms ATXPC; C7orf6; DRIF2; M7MLS1; UEF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152703
ORF Size 4752 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152703.5
RefSeq Size 7133 bp
RefSeq ORF 4755 bp
Locus ID 219285
UniProt ID Q8IVG5
Cytogenetics 7q21.2
MW 184.4 kDa
Summary This gene encodes a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. The encoded protein contains an N-terminal sterile alpha motif domain. Naturally occurring mutations in this gene are associated with myeloid disorders such as juvenile myelomonocytic leukemia, acute myeloid leukemia, and myelodysplastic syndrome. Naturally occurring mutations are also associated with hepatitis-B related hepatocellular carcinoma, normophosphatemic familial tumoral calcinosis, and ataxia-pancytopenia syndrome. provided by RefSeq, Apr 2017
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Documents
Resources
"NM_152703" in other vectors (4)
SKU Description Size Price
RC214427L3 Lenti-ORF clone of SAMD9L (Myc-DDK-tagged)-Human sterile alpha motif domain containing 9-like (SAMD9L) 10 ug
$2,332.00
RC214427L4 Lenti-ORF clone of SAMD9L (mGFP-tagged)-Human sterile alpha motif domain containing 9-like (SAMD9L) 10 ug
$2,332.00
RG214427 SAMD9L (tGFP-tagged) - Human sterile alpha motif domain containing 9-like (SAMD9L) 10 ug
$789.00 $2,232.00
SC309904 SAMD9L (untagged)-Human sterile alpha motif domain containing 9-like (SAMD9L) 10 ug
$2,169.00

Plasmid Map for RC214427

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Cloning scheme for RC214427

Circular map for RC214427

file-alt Citations

Germline SAMD9L truncation variants trigger global translational repression
Allenspach, Soveg, Finn et al
J Exp Med (2021) 218 (5) DOI: 10.1084/jem.20201195
Cited by 43
AI Summary: OriGene's human SAMD9L cDNA (GenBank accession no. NM_152703) was used in this study to generate expression constructs for functional analysis of SAMD9L truncation variants and their impact on global translational repression.
Snippet: … The vector had either no gene or the human SAMD9L (GenBank accession no. NM_152703) sequence (Origene) inserted downstream of the T2A and N-terminal 3xF using In-Fusion cloning (Takara Bio). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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