Integrin alpha 4 (ITGA4) (NM_000885) Human Tagged ORF Clone

SKU RC214408

ITGA4 (Myc-DDK-tagged)-Human integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Integrin alpha 4
• Synonyms: CD49D; IA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214408 representing NM_000885; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTTGGGAAGCGAGGCGCGAACCCGGCCCCCGAAGGGCCGCCGTCCGGGAGACGGTGATGCTGTTGC; TGTGCCTGGGGGTCCCGACCGGCCGCCCCTACAACGTGGACACTGAGAGCGCGCTGCTTTACCAGGGCCC; CCACAACACGCTGTTCGGCTACTCGGTCGTGCTGCACAGCCACGGGGCGAACCGATGGCTCCTAGTGGGT; GCGCCCACTGCCAACTGGCTCGCCAACGCTTCAGTGATCAATCCCGGGGCGATTTACAGATGCAGGATCG; GAAAGAATCCCGGCCAGACGTGCGAACAGCTCCAGCTGGGTAGCCCTAATGGAGAACCTTGTGGAAAGAC; TTGTTTGGAAGAGAGAGACAATCAGTGGTTGGGGGTCACACTTTCCAGACAGCCAGGAGAAAATGGATCC; ATCGTGACTTGTGGGCATAGATGGAAAAATATATTTTACATAAAGAATGAAAATAAGCTCCCCACTGGTG; GTTGCTATGGAGTGCCCCCTGATTTACGAACAGAACTGAGTAAAAGAATAGCTCCGTGTTATCAAGATTA; TGTGAAAAAATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCCAGTTTTTACACAAAGGATTTA; ATTGTGATGGGGGCCCCAGGATCATCTTACTGGACTGGCTCTCTTTTTGTCTACAATATAACTACAAATA; AATACAAGGCTTTTTTAGACAAACAAAATCAAGTAAAATTTGGAAGTTATTTAGGATATTCAGTCGGAGC; TGGTCATTTTCGGAGCCAGCATACTACCGAAGTAGTCGGAGGAGCTCCTCAACATGAGCAGATTGGTAAG; GCATATATATTCAGCATTGATGAAAAAGAACTAAATATCTTACATGAAATGAAAGGTAAAAAGCTTGGAT; CGTACTTTGGAGCTTCTGTCTGTGCTGTGGACCTCAATGCAGATGGCTTCTCAGATCTGCTCGTGGGAGC; ACCCATGCAGAGCACCATCAGAGAGGAAGGAAGAGTGTTTGTGTACATCAACTCTGGCTCGGGAGCAGTA; ATGAATGCAATGGAAACAAACCTCGTTGGAAGTGACAAATATGCTGCAAGATTTGGGGAATCTATAGTTA; ATCTTGGCGACATTGACAATGATGGCTTTGAAGATGTTGCTATCGGAGCTCCACAAGAAGATGACTTGCA; AGGTGCTATTTATATTTACAATGGCCGTGCAGATGGGATCTCGTCAACCTTCTCACAGAGAATTGAAGGA; CTTCAGATCAGCAAATCGTTAAGTATGTTTGGACAGTCTATATCAGGACAAATTGATGCAGATAATAATG; GCTATGTAGATGTAGCAGTTGGTGCTTTTCGGTCTGATTCTGCTGTCTTGCTAAGGACAAGACCTGTAGT; AATTGTTGACGCTTCTTTAAGCCACCCTGAGTCAGTAAATAGAACGAAATTTGACTGTGTTGAAAATGGA; TGGCCTTCTGTGTGCATAGATCTAACACTTTGTTTCTCATATAAGGGCAAGGAAGTTCCAGGTTACATTG; TTTTGTTTTATAACATGAGTTTGGATGTGAACAGAAAGGCAGAGTCTCCACCAAGATTCTATTTCTCTTC; TAATGGAACTTCTGACGTGATTACAGGAAGCATACAGGTGTCCAGCAGAGAAGCTAACTGTAGAACACAT; CAAGCATTTATGCGGAAAGATGTGCGGGACATCCTCACCCCAATTCAGATTGAAGCTGCTTACCACCTTG; GTCCTCATGTCATCAGTAAACGAAGTACAGAGGAATTCCCACCACTTCAGCCAATTCTTCAGCAGAAGAA; AGAAAAAGACATAATGAAAAAAACAATAAACTTTGCAAGGTTTTGTGCCCATGAAAATTGTTCTGCTGAT; TTACAGGTTTCTGCAAAGATTGGGTTTTTGAAGCCCCATGAAAATAAAACATATCTTGCTGTTGGGAGTA; TGAAGACATTGATGTTGAATGTGTCCTTGTTTAATGCTGGAGATGATGCATATGAAACGACTCTACATGT; CAAACTACCCGTGGGTCTTTATTTCATTAAGATTTTAGAGCTGGAAGAGAAGCAAATAAACTGTGAAGTC; ACAGATAACTCTGGCGTGGTACAACTTGACTGCAGTATTGGCTATATATATGTAGATCATCTCTCAAGGA; TAGATATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGAGCGGAAGAGGACCTCAGTATCACAGTGCA; TGCTACCTGTGAAAATGAAGAGGAAATGGACAATCTAAAGCACAGCAGAGTGACTGTAGCAATACCTTTA; AAATATGAGGTTAAGCTGACTGTTCATGGGTTTGTAAACCCAACTTCATTTGTGTATGGATCAAATGATG; AAAATGAGCCTGAAACGTGCATGGTGGAGAAAATGAACTTAACTTTCCATGTTATCAACACTGGCAATAG; TATGGCTCCCAATGTTAGTGTGGAAATAATGGTACCAAATTCTTTTAGCCCCCAAACTGATAAGCTGTTC; AACATTTTGGATGTCCAGACTACTACTGGAGAATGCCACTTTGAAAATTATCAAAGAGTGTGTGCATTAG; AGCAGCAAAAGAGTGCAATGCAGACCTTGAAAGGCATAGTCCAGTTCTTGTCCAAGACTGATAAGAGGCT; ATTGTACTGCATAAAAGCTGATCCACATTGTTTAAATTTCTTGTGTAATTTTGGGAAAATGGAAAGTGGA; AAAGAAGCCAGTGTTCATATCCAACTGGAAGGCCGGCCATCCATTTTAGAAATGGATGAGACTTCAGCAC; TCAAGTTTGAAATAAGAGCAACAGGTTTTCCAGAGCCAAATCCAAGAGTAATTGAACTAAACAAGGATGA; GAATGTTGCGCATGTTCTACTGGAAGGACTACATCATCAAAGACCCAAACGTTATTTCACCATAGTGATT; ATTTCAAGTAGCTTGCTACTTGGACTTATTGTACTTCTGTTGATCTCATATGTTATGTGGAAGGCTGGCT; TCTTTAAAAGACAATACAAATCTATCCTACAAGAAGAAAACAGAAGAGACAGTTGGAGTTATATCAACAG; TAAAAGCAATGATGAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214408 representing NM_000885; Red=Cloning site Green=Tags(s); ; MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLYQGPHNTLFGYSVVLHSHGANRWLLVG; APTANWLANASVINPGAIYRCRIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGS; IVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDL; IVMGAPGSSYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGK; AYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGSGAV; MNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQEDDLQGAIYIYNGRADGISSTFSQRIEG; LQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENG; WPSVCIDLTLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTH; QAFMRKDVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAHENCSAD; LQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEV; TDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPL; KYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLF; NILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVQFLSKTDKRLLYCIKADPHCLNFLCNFGKMESG; KEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVI; ISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000885
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000885.6
• RefSeq Size: 6082 bp
• RefSeq ORF: 3099 bp
• Locus ID: 3676
• UniProt ID: P13612
• Cytogenetics: 2q31.3
• Domains: FG-GAP
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• MW: 114.87 kDa
• Summary: The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]