KRTAP19-7 (NM_181614) Human Tagged ORF Clone

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SKU
RC214387
KRTAP19 (Myc-DDK-tagged)-Human keratin associated protein 19-7 (KRTAP19-7)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTAP19-7
Synonyms KAP19.7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214387 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACTCCGGCAGCTACTATGGAGGCCTAGGCTACGGCTGTGGAGGATTCGGTGGCCTGGGCTATG
GCTATAGCTGTGGATGTGGCAGCTTCCGCAGACTGGGCTATGGCTGTGGCTATGGAGGCTACAGATACAG
CTGCTGCCACCCATCATGCTATGGGGGATACTGGTCTTCTGGATTCTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214387 protein sequence
Red=Cloning site Green=Tags(s)

MSYSGSYYGGLGYGCGGFGGLGYGYSCGCGSFRRLGYGCGYGGYRYSCCHPSCYGGYWSSGFY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181614
ORF Size 189 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181614.1, NP_853645.1
RefSeq Size 192 bp
RefSeq ORF 192 bp
Locus ID 337974
UniProt ID Q3SYF9
Cytogenetics 21q22.11
Protein Families Transmembrane
MW 6.6 kDa
Summary In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181614" in other vectors (4)
SKU Description Size Price
RC214387L3 Lenti ORF clone of Human keratin associated protein 19-7 (KRTAP19-7), Myc-DDK-tagged 10 ug
$450.00
RC214387L4 Lenti ORF clone of Human keratin associated protein 19-7 (KRTAP19-7), mGFP tagged 10 ug
$450.00
RG214387 KRTAP19 (tGFP-tagged) - Human keratin associated protein 19-7 (KRTAP19-7) 10 ug
$489.00
SC307324 KRTAP19 (untagged)-Human keratin associated protein 19-7 (KRTAP19-7) 10 ug
$165.00

Plasmid Map for RC214387

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Cloning scheme for RC214387

Circular map for RC214387

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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