TAOK2 (NM_016151) Human Tagged ORF Clone
$1,612.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TAOK2 |
| Synonyms | MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016151 |
| ORF Size | 3705 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016151.4 |
| RefSeq Size | 4920 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 9344 |
| UniProt ID | Q9UL54 |
| Cytogenetics | 16p11.2 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | MAPK signaling pathway |
| MW | 138.1 kDa |
| Summary | This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Oct 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016151" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214297L1 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,912.00
|
|
| RC214297L2 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 2, mGFP tagged | 10 ug |
$1,912.00
|
|
| RC214297L3 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,912.00
|
|
| RC214297L4 | Lenti ORF clone of Human TAO kinase 2 (TAOK2), transcript variant 2, mGFP tagged | 10 ug |
$1,912.00
|
|
| RG214297 | TAOK2 (tGFP-tagged) - Human TAO kinase 2 (TAOK2), transcript variant 2 | 10 ug |
$1,812.00
|
|
| SC315289 | TAOK2 (untagged)-Human TAO kinase 2 (TAOK2), transcript variant 2 | 10 ug |
$1,721.00
|
|
Citations
TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules
Nourbakhsh, Ferreccio, Bernard et al
Dev Cell (2021) 56 (24), 3321-3333.e5 DOI: 10.1016/j.devcel.2021.11.015
Nourbakhsh, Ferreccio, Bernard et al
Dev Cell (2021) 56 (24), 3321-3333.e5 DOI: 10.1016/j.devcel.2021.11.015
Cited by 32
AI Summary: OriGene’s full-length human TAOK2 cDNA (isoform alpha) was used as the PCR template for generating TAOK2 constructs, enabling the study of TAOK2’s role in tethering the endoplasmic reticulum to microtubules.
Snippet: ) and 1% Pen-Strep (Invitrogen). Cells were maintained at 5% CO2 and 37°C and passaged every 3–4 days. METHODS DETAILS Molecular cloning Full-length human TAOK2 was PCR amplified from TAOK2 cDNA (Origene, isoform alpha), and inserted in vector sfGFP-C1 (Addgene #54579) using restriction sites HindIII and MfeI. Domain dissection mutants were subcloned from sfGFP-TAOK2 using restriction enzymes
Showing 1-1 of 1 papers.
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