CCRK (CDK20) (NM_001039803) Human Tagged ORF Clone

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SKU
RC214249
CDK20 (Myc-DDK-tagged)-Human cyclin-dependent kinase 20 (CDK20), transcript variant 3
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCRK
Synonyms CCRK; CDCH; P42; PNQALRE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214249 representing NM_001039803
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCAGTACTGCATCCTGGGCCGCATCGGGGAGGGCGCCCACGGCATCGTCTTCAAGGCCAAGCACG
TGGAGACTGGCGAGATAGTTGCCCTCAAGAAGGTGGCCCTAAGGCGGTTGGAGGACGGCTTCCCTAACCA
GGCCCTGCGGGAGATTAAGGCTCTGCAGGAGATGGAGGACAATCAGTATGTGGTACAACTGAAGGCTGTG
TTCCCACACGGTGGAGGCTTTGTGCTGGCCTTTGAGTTCATGCTGTCGGATCTGGCCGAGGTGGTGCGCC
ATGCCCAGAGGCCACTAGCCCAGGCACAGGTCAAGAGCTACCTGCAGATGCTGCTCAAGGGTGTCGCCTT
CTGCCATGCCAACAACATTGTACATCGGGACCTGAAACCTGCCAACCTGCTCATCAGCGCCTCAGGCCAG
CTCAAGATAGCGGACTTTGGCCTGGCTCGAGTCTTTTCCCCAGACGGCAGCCGCCTCTACACACACCAGG
TGGCCACCAGGTGGTACCGAGCCCCCGAGCTCCTGTATGGTGCCCGCCAGTATGACCAGGGCGTCGATCT
GTGGTCTGTGGGCTGCATCATGGGGGAGCTGTTGAATGGGTCCCCCCTTTTCCCGGGCAAGAACGATATT
GAACAGCTTTGCTATGTGCTTCGCATCTTGGGCACCCCAAACCCTCAAGTCTGGCCGGAGCTCACTGAGC
TGCCGGACTACAACAAGATCTCCTTTAAGGAGCAGGTGCCCATGCCCCTGGAGGAGGTGCTGCCTGACGT
CTCTCCCCAGGCATTGGATCTGCTGGGTCAATTCCTTCTCTACCCTCCTCACCAGCGCATCGCAGCTTCC
AAGGCTCTCCTCCATCAGTACTTCTTCACAGCTCCCCTGCCTGCCCATCCATCTGAGCTGCCGATTCCTC
AGCGTCTAGGGGGACCTGCCCCCAAGGCCCATCCAGGGCCCCCCCACATCCATGACTTCCACGTGGACCG
GCCTCTTGAGGAGTCGCTGTTGAACCCAGAGCTGATTCGGCCCTTCATCCTGGAGGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214249 representing NM_001039803
Red=Cloning site Green=Tags(s)

MDQYCILGRIGEGAHGIVFKAKHVETGEIVALKKVALRRLEDGFPNQALREIKALQEMEDNQYVVQLKAV
FPHGGGFVLAFEFMLSDLAEVVRHAQRPLAQAQVKSYLQMLLKGVAFCHANNIVHRDLKPANLLISASGQ
LKIADFGLARVFSPDGSRLYTHQVATRWYRAPELLYGARQYDQGVDLWSVGCIMGELLNGSPLFPGKNDI
EQLCYVLRILGTPNPQVWPELTELPDYNKISFKEQVPMPLEEVLPDVSPQALDLLGQFLLYPPHQRIAAS
KALLHQYFFTAPLPAHPSELPIPQRLGGPAPKAHPGPPHIHDFHVDRPLEESLLNPELIRPFILEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039803
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039803.3
RefSeq Size 2368 bp
RefSeq ORF 1041 bp
Locus ID 23552
UniProt ID Q8IZL9, A2A390
Cytogenetics 9q22.1
Protein Families Druggable Genome, Protein Kinase
MW 38.5 kDa
Summary The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase may activate cyclin-dependent kinase 2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Dec 2009
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Documents
Resources
"NM_001039803" in other vectors (4)
SKU Description Size Price
RC214249L3 Lenti ORF clone of Human cyclin-dependent kinase 20 (CDK20), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC214249L4 Lenti ORF clone of Human cyclin-dependent kinase 20 (CDK20), transcript variant 3, mGFP tagged 10 ug
$757.00
RG214249 CDK20 (tGFP-tagged) - Human cyclin-dependent kinase 20 (CDK20), transcript variant 3 10 ug
$657.00
SC311055 CDK20 (untagged)-Human cyclin-dependent kinase 20 (CDK20), transcript variant 3 10 ug
$503.00

Plasmid Map for RC214249

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Cloning scheme for RC214249

Circular map for RC214249

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