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DNAAF4 (NM_001033559) Human Tagged ORF Clone

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SKU
RC214248
DYX1C1 (Myc-DDK-tagged)-Human dyslexia susceptibility 1 candidate 1 (DYX1C1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "DNAAF4 or NM_001033559"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAAF4
Synonyms CILD25; DYX1; DYX1C1; DYXC1; EKN1; RD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214248 representing NM_001033559
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCTTCAGGTTAGCGATTACAGCTGGCAGCAGACGAAGACTGCGGTCTTTCTGTCTCTGCCCCTCA
AAGGCGTGTGCGTCAGAGACACGGACGTGTTCTGCACGGAAAACTATCTGAAGGTCAACTTTCCTCCATT
TTTATTTGAGGCATTTCTTTATGCTCCCATAGACGATGAGAGCAGCAAAGCAAAGATTGGGAATGACACC
ATTGTCTTCACCTTGTATAAAAAAGAAGCGGCCATGTGGGAGACCCTTTCTGTGACGGGTGTTGACAAAG
AGATGATGCAAAGAATTAGAGAAAAATCTATTTTACAAGCACAAGAGAGAGCAAAAGAAGCTACAGAAGC
AAAAGCTGCAGCAAAGCGGGAAGATCAAAAATACGCACTAAGTGTCATGATGAAGATTGAAGAAGAAGAG
AGGAAAAAAATAGAAGATATGAAAGAAAATGAACGGATAAAAGCCACTAAAGCATTGGAAGCCTGGAAAG
AATATCAAAGAAAAGCTGAGGAGCAAAAAAAAATTCAGAGAGAAGAGAAATTATGTCAAAAAGAAAAGCA
AATTAAAGAAGAAAGAAAAAAAATAAAATATAAGAGTCTTACTAGAAATTTGGCATCTAGAAATCTTGCT
CCAAAAGGGAGAAATTCAGAAAATATATTTACTGAGAAGTTAAAGGAAGACAGTATTCCTGCTCCTCGCT
CTGTTGGCAGTATTAAAATCAACTTTACCCCTCGAGTATTCCCAACAGCTCTTCGTGAATCACAAGTAGC
AGAAGAGGAGGAGTGGCTACACAAACAAGCTGAGGCACGAAGAGCAATGAATACTGACATAGCTGAACTT
TGCGATTTAAAAGAAGAAGAAAAGAACCCAGAATGGTTGAAGGATAAAGGAAACAAATTGTTTGCAACGG
AAAACTATTTGGCAGCTATCAATGCATATAATTTAGCCATAAGACTAAATAATAAGATGCCACTATTGTA
TTTGAACCGGGCTGCTTGCCACCTAAAACTAAAAAACTTACACAAGGCTATTGAAGATTCTTCTAAGGCC
TACAGGATTATGAAGCGGCACTTAAGATTGATCCATCCAACAAAATTGTACAAATTGATGCTGAGAAGAT
TCGGAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214248 representing NM_001033559
Red=Cloning site Green=Tags(s)

MPLQVSDYSWQQTKTAVFLSLPLKGVCVRDTDVFCTENYLKVNFPPFLFEAFLYAPIDDESSKAKIGNDT
IVFTLYKKEAAMWETLSVTGVDKEMMQRIREKSILQAQERAKEATEAKAAAKREDQKYALSVMMKIEEEE
RKKIEDMKENERIKATKALEAWKEYQRKAEEQKKIQREEKLCQKEKQIKEERKKIKYKSLTRNLASRNLA
PKGRNSENIFTEKLKEDSIPAPRSVGSIKINFTPRVFPTALRESQVAEEEEWLHKQAEARRAMNTDIAEL
CDLKEEEKNPEWLKDKGNKLFATENYLAAINAYNLAIRLNNKMPLLYLNRAACHLKLKNLHKAIEDSSKA
YRIMKRHLRLIHPTKLYKLMLRRFGM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033559
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033559.2, NP_001028731.1
RefSeq Size 1887 bp
RefSeq ORF 1131 bp
Locus ID 161582
UniProt ID Q8WXU2
Cytogenetics 15q21.3
MW 43.9 kDa
Summary This gene encodes a tetratricopeptide repeat domain-containing protein. The encoded protein interacts with estrogen receptors and the heat shock proteins, Hsp70 and Hsp90. An homologous protein in rat has been shown to function in neuronal migration in the developing neocortex. A chromosomal translocation involving this gene is associated with a susceptibility to developmental dyslexia. Mutations in this gene are associated with deficits in reading and spelling. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream cell cycle progression 1 (CCPG1) gene. provided by RefSeq, Mar 2011
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"NM_001033559" in other vectors (4)
SKU Description Size Price
RC214248L3 Lenti ORF clone of Human dyslexia susceptibility 1 candidate 1 (DYX1C1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC214248L4 Lenti ORF clone of Human dyslexia susceptibility 1 candidate 1 (DYX1C1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG214248 DYX1C1 (tGFP-tagged) - Human dyslexia susceptibility 1 candidate 1 (DYX1C1), transcript variant 2 10 ug
$657.00
SC302728 DYX1C1 (untagged)-Human dyslexia susceptibility 1 candidate 1 (DYX1C1), transcript variant 2 10 ug
$503.00

Plasmid Map for RC214248

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214248
Circular map for RC214248

file-alt Citations

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