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LONRF3 (NM_001031855) Human Tagged ORF Clone

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SKU
RC214236
LONRF3 (Myc-DDK-tagged)-Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $695.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LONRF3
Synonyms RNF127
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214236 representing NM_001031855
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAGTACGGATCGAACAGATGCTGAGCTTGCCCGCCGAGGTCAGCAGCGACAACTTGGAGTCGG
CGGAGCGAGGGGCATCAGCGGCCCAAGTAGACATGGGCCCCCACCCAAAGGTGGCTGCAGAGGGCCCCGC
ACCTCTACCGACGCGGGAGCCAGAGCAAGAGCAGTCTCCGGGGACCTCAACGCCGGAGAGCAAAGTCCTG
CTCACGCAGGCAGACGCCTTGGCGTCCCGGGGGCGAATCCGTGAAGCCCTCGAGGTGTATAGACAGCTCT
CCGAGCGGCAGCAGCTGGTGGCTGAGCAGCTGGAGCAGCTGGTGCGCTGCCTGGCGGAGAAAGTCCCGCA
AGGCGAGGCGCTGGCGCCGGCGCCCCCGGACGAGGGTAGCACTGCAAGCGGCACCGTGGCGGCGGAAGAG
ACGGGGGCCGCCGCGGCTGCGGCGGCCACCGAGGTGTGGGACGGCTTTAAGTGCCGGAAATGTCATGGGT
TTCTATCAGACCCCGTGTCCTTGTCGTGTGGCCACACCTTTTGTAAACTGTGCCTGGAACGTGGGCGGGC
CGCCGACCGGCGCTGTGCGCTGTGCGGGGTCAAGCTCTCCGCCTTGATGGTGGCCACTGGGCGGGCGCGT
GGAGCCCGGCGGGCTGGGCAGCAGCCGCCGCCGCCGCTGCGAGTCAACGTGGTGCTCAGCGGCCTCCTCG
GCAAGTTGTTTCCAGGCCCAGCGCGAGCGTCGCAACTCCGGCACGAGGGCAACCGACTGTACCGCGAGCG
CCAGGTGGAGGCGGCACTGCTCAAGTACAACGAGGCAGTTAAGTTGGCTCCAAATGACCACTTGCTTTAT
AGCAATCGGTCTCAGATTTATTTCACCTTGGAGTCTCATGAGAATGCACTGCATGATGCAGAAATAGCAT
GTAAGCTCCGCCCGATGGGTTTTAAGGCACATTTCAGAAAAGCCCAAGCCTTAGCCACCCTAGGCAAGGT
GGAGGAGGCACTAAGGGAGTTTCTCTACTGTGTATCCCTTGATGGAAAGAACAAGAGAGCAAGATGTGAA
GCCCAAAGAGACAATCTGGAGCTCCCACATTGTTCTAGTCAGGAGGAAGCAGCAGCCAGGGGAGATGGCA
GCAGTCTGATGGACCCAGCTAAAGTGAAGGGGGATGGTCAGCAGCACCACATGAAAGACCAGGAAGAAGA
GGAGGAGAAGTGGGATGCTACCTCTCCAAAAGCTGCTTCCAGCAAGACTGGAAAATGCCAGGAAAAGAAA
AGGAAACATTGCCAGATTGAATCCCAAGAAGAAACGGGGATGCCTAATAAAGCCTCCAAGCAAGATCCTC
CCACTGATCAGGGGGACAAACCTGCTCTCAGTTTACCACTTGCATCTTTCGACGCATCTGACCTTGAATG
CGCTCTATGTATGAGATTATTCTATGAGCCAGTCACAACACCTTGCGGGCATACTTTTTGCTTAAAATGC
CTAGAAAGATGCCTAGATCACAACGCAAAGTGTCCATTGTGCAAAGACGGTCTTTCACAGTGCTTGGCAT
CAAGAAAATACAGCAAAAATGTAATAATGGAGGAGCTCATAGCTAAATTCCTTCCAGAAGAACTGAAGGA
ACGAAGGAAGCTTTATGAAGAGGAAATGGAAGAACTTTCTAACCTTAATAAGAATGTGCCTATTTTCGTG
TGTACTATGGCCTATCCCACCGTTCCTTGTCCCCTGCACATCTTTGAGCCTTGTTACCGCCTGATGATTC
GTAGATGCATTGAGACAGGCACGAGACAGTTTGGCATGTGCCTTGGAGATCCTGTCAAAGGGTTTGCAGA
ATATGGCTGCATCCTAGAGATCAGAAATGTTCAATTCCTTGCTGATGGCCGCTCAGTGGTTGACAGCATA
GGCAAGAGGCGCTTCAGGGTGCTCCATCAGAGCCAGCGGGATGGCTACAACACAGCCGACATTGAATACA
TTGAAGACCAAAAGGTTCAGGGAGAGGATTGTGCTGAGCTCATGGGATTACATAACTGTGTCTATCAGCA
AGCATCATTGTGGTTTCATTCGCTCAAATTATCCCTAAAGAATCGGATACTCAATCACTTTGGTCCCATG
CCGGAGAAAGACGCCGATCCTCAGATGAACCCGAATGGCCCAGCCTGGTGCTGGTGGATGTTAGCAGTTC
TTCCCTTGGAAAGCCGAGCTCAGCTCCCCTTCCTAGCAATGAGGTCCTTAAAGGACAGACTGAATGGTAT
TCGACGAGTCCTGGCCTTCATATCCCGAAACCAAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214236 representing NM_001031855
Red=Cloning site Green=Tags(s)

MESVRIEQMLSLPAEVSSDNLESAERGASAAQVDMGPHPKVAAEGPAPLPTREPEQEQSPGTSTPESKVL
LTQADALASRGRIREALEVYRQLSERQQLVAEQLEQLVRCLAEKVPQGEALAPAPPDEGSTASGTVAAEE
TGAAAAAAATEVWDGFKCRKCHGFLSDPVSLSCGHTFCKLCLERGRAADRRCALCGVKLSALMVATGRAR
GARRAGQQPPPPLRVNVVLSGLLGKLFPGPARASQLRHEGNRLYRERQVEAALLKYNEAVKLAPNDHLLY
SNRSQIYFTLESHENALHDAEIACKLRPMGFKAHFRKAQALATLGKVEEALREFLYCVSLDGKNKRARCE
AQRDNLELPHCSSQEEAAARGDGSSLMDPAKVKGDGQQHHMKDQEEEEEKWDATSPKAASSKTGKCQEKK
RKHCQIESQEETGMPNKASKQDPPTDQGDKPALSLPLASFDASDLECALCMRLFYEPVTTPCGHTFCLKC
LERCLDHNAKCPLCKDGLSQCLASRKYSKNVIMEELIAKFLPEELKERRKLYEEEMEELSNLNKNVPIFV
CTMAYPTVPCPLHIFEPCYRLMIRRCIETGTRQFGMCLGDPVKGFAEYGCILEIRNVQFLADGRSVVDSI
GKRRFRVLHQSQRDGYNTADIEYIEDQKVQGEDCAELMGLHNCVYQQASLWFHSLKLSLKNRILNHFGPM
PEKDADPQMNPNGPAWCWWMLAVLPLESRAQLPFLAMRSLKDRLNGIRRVLAFISRNQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001031855
ORF Size 2277 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001031855.3
RefSeq Size 2625 bp
RefSeq ORF 2280 bp
Locus ID 79836
UniProt ID Q496Y0, A8K2D3
Cytogenetics Xq24
Protein Families Druggable Genome, Protease
MW 84.3 kDa
Summary The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. provided by RefSeq, Jul 2008
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"NM_001031855" in other vectors (6)
SKU Description Size Price
RC214236L1 Lenti ORF clone of Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1, Myc-DDK-tagged 10 ug
$995.00
RC214236L2 Lenti ORF clone of Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1, mGFP tagged 10 ug
$995.00
RC214236L3 Lenti ORF clone of Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1, Myc-DDK-tagged 10 ug
$995.00
RC214236L4 Lenti ORF clone of Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1, mGFP tagged 10 ug
$995.00
RG214236 LONRF3 (tGFP-tagged) - Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1 10 ug
$789.00 $895.00
SC302615 LONRF3 (untagged)-Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1 10 ug
$696.00

Plasmid Map for RC214236

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214236
Circular map for RC214236

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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