OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Protor 1 (PRR5) (NM_001017529) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC214138
PRR5 (Myc-DDK-tagged)-Human proline rich 5 (renal) (PRR5), transcript variant 5
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA319960 Rabbit Polyclonal PRR5 Antibody
100 ug
$640.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Protor 1 or NM_001017529"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Protor 1
Synonyms FLJ20185k; PP610; PROTOR-1; PROTOR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214138 representing NM_001017529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATCCTTCGGGACAAGATTCGCTTCTATGAGGGACAGAAGCTGCTGGACTCACTGGCAGAGACCT
GGGACTTCTTCTTCAGTGACGTGCTGCCCATGCTGCAGGCCATCTTCTACCCGGTGCAGGGCAAGGAGCC
ATCGGTGCGCCAGCTGGCCCTGCTGCACTTCCGGAATGCCATCACCCTCAGTGTGAAGCTAGAGGATGCG
CTGGCCCGGGCCCATGCCCGTGTGCCCCCTGCCATCGTGCAGATGCTGCTGGTGCTGCAGGGGGTACATG
AGTCCAGGGGCGTGACTGAGGACTACCTGCGCCTGGAGACGCTGGTCCAGAAGGTGGTGTCGCCATACCT
GGGCACCTACGGCCTCCACTCCAGCGAGGGGCCCTTCACCCATTCCTGCATCCTGGAAAAGCGCCTCCTC
CGCCGCTCCCGCTCGGGGGACGTGCTGGCCAAGAACCCTGTGGTGCGCTCCAAGAGCTACAACACGCCTC
TGCTGAACCCCGTGCAGGAGCACGAGGCGGAGGGCGCGGCGGCCGGCGGTACCAGCATCCGCAGGCACTC
TGTGTCGGAGATGACGTCCTGCCCCGAGCCTCAGGGCTTCTCCGACCCGCCCGGCCAGGGCCCCACCGGG
ACCTTCAGGTCCTCCCCGGCGCCCCACTCAGGGCCCTGCCCCAGCAGACTGTACCCCACGACCCAGCCCC
CTGAGCAGGGCTTGGATCCCACCCGCAGCTCCCTGCCCCGCTCCAGCCCGGAGAACCTGGTGGACCAGAT
CCTGGAGTCCGTGGACTCGGATTCTGAAGGGATTTTCATTGACTTTGGCCGGGGCCGGGGCTCTGGCATG
TCCGACTTGGAGGGCTCTGGGGGCCGGCAGAGTGTCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214138 representing NM_001017529
Red=Cloning site Green=Tags(s)

MVILRDKIRFYEGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLALLHFRNAITLSVKLEDA
LARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLHSSEGPFTHSCILEKRLL
RRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGTSIRRHSVSEMTSCPEPQGFSDPPGQGPTG
TFRSSPAPHSGPCPSRLYPTTQPPEQGLDPTRSSLPRSSPENLVDQILESVDSDSEGIFIDFGRGRGSGM
SDLEGSGGRQSVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001017529
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001017529.2, NP_001017529.1
RefSeq Size 1560 bp
RefSeq ORF 882 bp
Locus ID 55615
UniProt ID P85299
Cytogenetics 22q13.31
MW 31.9 kDa
Summary This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. provided by RefSeq, Nov 2010
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001017529" in other vectors (4)
SKU Description Size Price
RC214138L3 Lenti ORF clone of Human proline rich 5 (renal) (PRR5), transcript variant 5, Myc-DDK-tagged 10 ug
$600.00
RC214138L4 Lenti ORF clone of Human proline rich 5 (renal) (PRR5), transcript variant 5, mGFP tagged 10 ug
$600.00
RG214138 PRR5 (tGFP-tagged) - Human proline rich 5 (renal) (PRR5), transcript variant 5 10 ug
$500.00
SC302064 PRR5 (untagged)-Human proline rich 5 (renal) (PRR5), transcript variant 5 10 ug
$330.00

Plasmid Map for RC214138

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214138
Circular map for RC214138

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products