DNAJB13 (NM_153614) Human Tagged ORF Clone

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SKU
RC214119
DNAJB13 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 13 (DNAJB13)
  $300.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJB13
Synonyms CILD34; RSPH16A; TSARG5; TSARG6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214119 representing NM_153614
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCAGGATTATTACTCTGTGCTCGGGATCACTCGCAATTCAGAGGATGCCCAGATCAAGCAGGCGT
ACCGCAGACTCGCCCTTAAGCACCACCCGTTGAAGTCAAATGAGCCGTCTTCAGCAGAGATTTTCAGGCA
AATAGCAGAGGCCTACGACGTGCTGAGTGACCCCATGAAGAGAGGCATCTACGACAAGTTTGGAGAAGAG
GGCCTGAAGGGTGGGATTCCTTTGGAGTTTGGATCCCAGACCCCATGGACAACTGGTTACGTCTTCCACG
GCAAACCTGAAAAGGTGTTCCACGAGTTCTTTGGTGGAAACAACCCCTTCAGTGAGTTTTTTGATGCAGA
AGGAAGTGAGGTAGATTTGAACTTTGGGGGGCTCCAGGGCCGAGGGGTCAAGAAGCAGGACCCCCAAGTC
GAACGGGATCTCTACCTGTCCCTGGAGGACTTATTCTTTGGCTGCACCAAAAAAATTAAGATCTCCAGAA
GGGTGCTGAACGAGGATGGGTACTCCTCCACCATCAAGGACAAGATCCTGACCATTGATGTGAAGCCCGG
TTGGAGGCAGGGCACACGCATCACCTTTGAGAAGGAAGGGGACCAGGGCCCCAACATCATCCCAGCAGAC
ATCATTTTCATCGTAAAGGAGAAGCTACACCCTCGCTTCCGCAGGGAGAATGACAACCTCTTCTTCGTGA
ACCCCATCCCTCTTGGCAAGGCTCTCACCTGCTGCACTGTGGAGGTGAGGACCCTAGATGACCGTCTGCT
CAACATCCCCATCAATGACATCATCCACCCCAAATACTTCAAGAAGGTGCCAGGGGAGGGGATGCCATTG
CCGGAGGACCCCACTAAGAAAGGGGATCTCTTCATCTTCTTCGACATCCAGTTCCCCACCCGCCTCACAC
CCCAGAAGAAGCAGATGCTGCGCCAGGCATTGCTGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214119 representing NM_153614
Red=Cloning site Green=Tags(s)

MGQDYYSVLGITRNSEDAQIKQAYRRLALKHHPLKSNEPSSAEIFRQIAEAYDVLSDPMKRGIYDKFGEE
GLKGGIPLEFGSQTPWTTGYVFHGKPEKVFHEFFGGNNPFSEFFDAEGSEVDLNFGGLQGRGVKKQDPQV
ERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQGTRITFEKEGDQGPNIIPAD
IIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVRTLDDRLLNIPINDIIHPKYFKKVPGEGMPL
PEDPTKKGDLFIFFDIQFPTRLTPQKKQMLRQALLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153614
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153614.2, NP_705842.2
RefSeq Size 1875 bp
RefSeq ORF 951 bp
Locus ID 374407
UniProt ID P59910
Cytogenetics 11q13.4
MW 35.9 kDa
Summary This gene encodes a member of the heat shock protein 40 co-chaperone family which is produced in large amounts in the testis and is located on the radial spokes of the axoneme in human sperm flagella and other flagellar structures. The encoded protein associates with the sperm annulus, as part of the septin complex, through direct interaction with septin 4, during sperm terminal differentiation. Naturally occurring mutations in this gene are associated with primary ciliary dyskinesia and male infertility. provided by RefSeq, Apr 2017
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Documents
Resources
"NM_153614" in other vectors (4)
SKU Description Size Price
RC214119L3 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 13 (DNAJB13), Myc-DDK-tagged 10 ug
$600.00
RC214119L4 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 13 (DNAJB13), mGFP tagged 10 ug
$600.00
RG214119 DNAJB13 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 13 (DNAJB13) 10 ug
$500.00
SC306626 DNAJB13 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 13 (DNAJB13) 10 ug
$330.00

Plasmid Map for RC214119

Download sequence for

Cloning scheme for RC214119

Circular map for RC214119

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