FMO5 (NM_001461) Human Tagged ORF Clone

SKU RC214057

FMO5 (Myc-DDK-tagged)-Human flavin containing monooxygenase 5 (FMO5), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FMO5
• Synonyms: hBVMO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214057 representing NM_001461; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACTAAGAAAAGAATTGCTGTGATTGGGGGAGGAGTGAGCGGGCTCTCTTCCATCAAGTGCTGCGTAG; AAGAAGGCTTGGAACCTGTCTGCTTTGAAAGGACTGATGACATCGGAGGGCTCTGGAGGTTCCAGGAAAA; TCCTGAAGAAGGAAGGGCCAGTATTTACAAATCAGTGATCATCAATACTTCTAAAGAGATGATGTGCTTC; AGTGACTATCCAATCCCAGATCATTATCCCAACTTCATGCATAATGCCCAGGTCCTGGAGTATTTCAGGA; TGTATGCCAAAGAATTTGACCTTCTAAAGTATATTCGATTTAAGACCACTGTGTGCAGTGTGAAGAAGCA; GCCTGATTTTGCCACTTCAGGCCAATGGGAAGTGGTCACTGAATCTGAAGGGAAAAAGGAGATGAATGTC; TTTGATGGAGTCATGGTTTGCACTGGCCATCACACCAATGCTCATCTACCTCTGGAAAGCTTCCCTGGAA; TTGAGAAGTTCAAAGGGCAGTACTTCCACAGTCGAGACTATAAGAACCCAGAGGGATTCACTGGAAAGAG; AGTCATTATAATTGGCATTGGGAATTCTGGAGGGGATCTGGCTGTAGAGATTAGCCAAACAGCCAAGCAG; GTTTTCCTCAGCACCAGGAGAGGGGCTTGGATCCTGAATCGTGTAGGGGACTACGGATATCCTGCTGATG; TGTTGTTCTCTTCTCGACTTACACATTTTATATGGAAGATCTGTGGCCAATCATTAGCAAACAAATATTT; GGAAAAAAAGATAAACCAAAGGTTTGACCATGAAATGTTTGGCCTGAAGCCTAAACACAGAGCTCTGAGT; CAGCATCCAACCTTAAATGATGACCTGCCAAATCGTATCATTTCTGGCTTGGTGAAAGTGAAAGGAAATG; TGAAGGAATTCACGGAGACAGCTGCCATATTTGAGGATGGCTCCAGGGAGGATGACATTGATGCTGTTAT; CTTTGCCACAGGCTATAGCTTTGACTTTCCRTTTCTGGAAGATTCCGTCAAAGTGGTCAAAAACAAGATA; TCCCTGTATAAAAAGGTCTTCCCTCCTAACCTGGAAAGGCCAACTCTTGCAATCATAGGCTTGATTCAGC; CCTTAGGAGCCATTATGCCCATTTCAGAGCTCCAAGGACGCTGGGCCACTCAGGTATTTAAAGGTCTAAA; GACATTGCCCTCACAGAGTGAAATGATGGCAGAAATATCTAAAGCTCAAGAGGAAATTGACAAAAGGTAT; GTGGAGAGCCAACGCCATACCATTCAGGGAGACTACATAGATACCATGGAAGAGCTTGCTGATTTGGTGG; GGGTCAGGCCCAATCTGCTGTCTCTGGCCTTCACTGACCCCAAGCTGGCATTACACTTATTACTGGGACC; CTGCACTCCAATCCACTATCGTGTACAGGGCCCTGGAAAGTGGGATGGGGCTCGAAAAGCTATCCTCACC; ACAGATGATCGCATCAGGAAGCCTCTGATGACAAGAGTAGTTGAAAGGAGTAGTTCTATGACTTCAACAA; TGACAATAGGCAAGTTTATGCTAGCTCTTGCCTTCTTTGCTATAATTATAGCTTACTTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001461
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001461.4
• RefSeq Size: 2326 bp
• RefSeq ORF: 1602 bp
• Locus ID: 2330
• UniProt ID: P49326
• Cytogenetics: 1q21.1
• Domains: FMO-like
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450
• MW: 60 kDa
• Summary: Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]