KIF4A (NM_012310) Human Tagged ORF Clone

SKU RC213971

KIF4A (Myc-DDK-tagged)-Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KIF4A
• Synonyms: KIF4; KIF4G1; MRX100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213971 ORF sequence, codon optimized.; Due to the complexity of NM_012310, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGGAAGAGGTCAAGGGCATACCCGTGCGAGTTGCGTTGCGCTGTCGCCCACTGGTGCCAAAGGAAA; TTTCTGAGGGATGCCAGATGTGTCTCTCCTTTGTCCCGGGGGAGCCGCAGGTCGTGGTCGGCACTGACAA; GAGCTTTACATACGATTTCGTTTTTGACCCTAGCACAGAGCAGGAGGAGGTATTTAATACCGCGGTCGCA; CCCCTCATAAAGGGAGTGTTTAAGGGCTACAACGCCACCGTGTTGGCTTACGGCCAGACCGGCAGTGGCA; AGACATATTCTATGGGTGGGGCATACACCGCAGAACAGGAGAACGAACCAACCGTGGGGGTAATTCCTCG; AGTAATCCAACTTCTTTTTAAGGAGATCGATAAAAAGAGTGACTTCGAATTTACCCTCAAAGTTAGTTAT; CTGGAGATCTACAACGAGGAAATCCTGGATCTGCTGTGTCCCAGCAGGGAGAAAGCCCAGATCAATATTA; GGGAGGACCCCAAGGAGGGGATCAAGATAGTGGGGCTGACCGAAAAGACAGTGCTCGTTGCTCTGGATAC; CGTGAGCTGTTTGGAACAGGGGAACAATTCACGCACTGTCGCGTCCACCGCCATGAACTCTCAGTCTTCC; CGCTCTCACGCAATTTTCACCATCAGTCTTGAGCAGCGGAAAAAGAGTGACAAGAATAGCAGCTTTAGGT; CCAAGCTGCATCTGGTGGATTTGGCCGGCAGCGAGCGGCAAAAAAAGACCAAGGCTGAGGGCGACCGGCT; GAAAGAGGGTATTAACATCAATCGGGGACTTCTGTGCTTGGGAAATGTAATCTCAGCCCTGGGTGACGAT; AAGAAAGGAGGATTCGTACCATACCGCGACTCAAAACTCACCAGACTGCTGCAGGACTCACTGGGAGGAA; ACTCACACACTCTGATGATCGCGTGTGTGTCTCCTGCTGATAGCAATCTCGAAGAAACTCTCAATACCCT; GCGCTACGCCGACCGGGCACGCAAGATCAAAAACAAACCTATAGTGAACATCGACCCTCAGACAGCTGAG; CTCAACCACCTGAAGCAGCAAGTGCAGCAGCTGCAGGTCTTGCTGCTGCAGGCCCACGGCGGCACGCTGC; CTGGCAGCATTACTGTTGAACCAAGTGAAAACCTGCAGTCCTTGATGGAGAAGAACCAGTCTCTCGTTGA; GGAAAACGAAAAGCTCAGCCGAGGGCTTTCCGAGGCCGCCGGTCAAACAGCACAAATGCTCGAAAGGATT; ATTCTCACCGAGCAGGCCAATGAAAAGATGAACGCAAAACTTGAGGAGCTGAGACAGCACGCCGCCTGTA; AACTGGACCTGCAAAAGCTGGTGGAGACTCTGGAGGACCAGGAGCTGAAGGAAAACGTGGAGATCATATG; TAATCTGCAGCAACTGATCACACAGTTGAGCGACGAGACAGTTGCCTGTATGGCTGCTGCCATCGACACG; GCAGTGGAGCAGGAAGCTCAGGTTGAGACTAGCCCCGAAACTAGCCGCAGTTCCGATGCGTTCACAACCC; AGCACGCATTGCGACAGGCTCAGATGAGTAAGGAATTGGTGGAGTTGAACAAAGCTCTGGCTCTGAAGGA; GGCCTTGGCGAGGAAGATGACCCAGAATGACTCCCAGCTGCAGCCCATCCAGTATCAGTACCAAGACAAC; ATCAAAGAACTCGAGCTTGAAGTGATCAACCTTCAGAAGGAAAAAGAAGAGCTGGTCCTGGAGCTTCAAA; CTGCAAAGAAAGATGCAAACCAGGCCAAACTGAGTGAAAGGCGACGCAAGAGATTGCAGGAGCTGGAGGG; TCAGATTGCAGACCTGAAGAAAAAACTGAACGAACAGTCCAAGCTCCTGAAACTGAAGGAGAGCACAGAG; CGGACCGTCTCAAAGCTGAATCAGGAGATCCGCATGATGAAAAACCAACGGGTACAGTTGATGCGGCAAA; TGAAGGAAGACGCAGAGAAGTTCAGGCAGTGGAAGCAGAAAAAGGACAAAGAAGTGATCCAGCTGAAGGA; AAGGGACCGGAAGAGGCAGTACGAGCTTCTCAAACTGGAGCGGAACTTTCAAAAACAGTCCAATGTCCTG; CGGCGGAAAACCGAGGAAGCAGCAGCCGCAAACAAGCGGCTGAAAGATGCCTTGCAGAAACAGCGGGAAG; TTGCGGACAAGCGAAAAGAGACTCAGAGCCGGGGCATGGAGGGAACCGCCGCTCGCGTAAAGAACTGGCT; GGGCAATGAAATAGAGGTGATGGTGAGCACCGAGGAGGCCAAACGCCACCTCAATGACCTCCTCGAAGAT; CGGAAAATCCTTGCACAAGACGTGGCCCAACTGAAGGAGAAAAAAGAATCCGGCGAAAACCCTCCCCCTA; AGTTGCGGCGACGAACATTCAGCCTCACAGAGGTAAGGGGCCAGGTTAGCGAATCAGAAGACAGCATCAC; GAAACAGATCGAATCCTTGGAAACCGAGATGGAATTTAGATCCGCACAGATCGCCGACCTGCAGCAAAAG; CTCCTGGACGCCGAGAGTGAGGACAGGCCTAAACAAAGGTGGGAGAACATTGCTACAATACTCGAAGCCA; AATGTGCCCTTAAATACTTGATCGGCGAGCTGGTTAGCAGCAAAATACAGGTGAGTAAGCTTGAGAGTTC; TCTCAAGCAGAGCAAAACCTCCTGCGCAGATATGCAGAAAATGCTGTTCGAGGAAAGAAATCATTTCGCC; GAGATTGAAACCGAGTTGCAAGCTGAGCTGGTTAGGATGGAACAGCAGCATCAGGAGAAAGTCCTGTATC; TCCTGTCACAGCTCCAACAGAGCCAAATGGCAGAGAAGCAGCTGGAAGAGTCAGTGAGTGAGAAGGAGCA; GCAGCTTCTGAGCACCCTGAAATGCCAAGACGAAGAACTCGAAAAGATGCGCGAAGTGTGCGAGCAGAAT; CAGCAGCTCCTCAGGGAAAACGAAATCATTAAACAAAAACTCACATTGCTGCAGGTGGCTAGCAGACAGA; AGCACTTGCCAAAAGATACGCTGCTGAGCCCAGACAGCTCTTTCGAGTATGTGCCTCCAAAACCTAAACC; ATCCCGCGTGAAAGAGAAGTTTCTGGAACAGAGCATGGATATCGAGGATTTGAAGTATTGCTCCGAGCAT; TCAGTCAATGAACATGAGGACGGTGATGGTGACGACGACGAGGGAGATGATGAAGAATGGAAACCGACCA; AACTCGTCAAAGTTAGCCGCAAAAATATCCAAGGCTGTTCATGCAAAGGATGGTGTGGCAACAAGCAATG; CGGTTGCAGGAAACAGAAATCTGATTGCGGTGTAGACTGTTGTTGCGATCCTACAAAATGCCGGAATCGC; CAACAGGGAAAAGACAGCCTCGGTACAGTTGAGAGAACCCAGGATTCAGAGGGAAGCTTCAAACTCGAGG; ATCCCACAGAGGTTACCCCAGGCCTGAGTTTCTTCAATCCCGTGTGCGCCACACCTAATAGTAAGATTCT; GAAGGAGATGTGCGACGTGGAACAGGTTCTTTCCAAAAAAACCCCTCCCGCCCCATCTCCCTTTGATCTG; CCTGAACTTAAGCATGTTGCTACTGAGTACCAAGAAAATAAGGCCCCTGGTAAGAAGAAGAAACGGGCTC; TGGCCTCTAATACCTCCTTTTTCTCTGGCTGTTCCCCAATTGAGGAGGAGGCCCAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213971 representing NM_012310; Red=Cloning site Green=Tags(s); ; MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVA; PLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSDFEFTLKVSY; LEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSS; RSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDD; KKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAE; LNHLKQQVQQLQVLLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERI; ILTEQANEKMNAKLEELRQHAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDT; AVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQDN; IKELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQSKLLKLKESTE; RTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVL; RRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTAARVKNWLGNEIEVMVSTEEAKRHLNDLLED; RKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQK; LLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEERNHFA; EIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEELEKMREVCEQN; QQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIEDLKYCSEH; SVNEHEDGDGDDDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGCRKQKSDCGVDCCCDPTKCRNR; QQGKDSLGTVERTQDSEGSFKLEDPTEVTPGLSFFNPVCATPNSKILKEMCDVEQVLSKKTPPAPSPFDL; PELKHVATEYQENKAPGKKKKRALASNTSFFSGCSPIEEEAH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012310
• ORF Size: 3696 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012310.3, NM_012310.4, NP_036442.3
• RefSeq Size: 4388 bp
• RefSeq ORF: 3699 bp
• Locus ID: 24137
• UniProt ID: O95239
• Cytogenetics: Xq13.1
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 139.9 kDa
• Summary: This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]