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FBXO3 (NM_033406) Human Tagged ORF Clone

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SKU
RC213872
FBXO3 (Myc-DDK-tagged)-Human F-box protein 3 (FBXO3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO3
Synonyms FBA; FBX3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213872 representing NM_033406
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCATGGAGACCGAGACGGCGCCGCTGACCCTAGAGTCGCTGCCCACCGATCCCCTGCTCCTCA
TCTTATCCTTTTTGGACTATCGGGATCTAATCAACTGTTGTTATGTCAGTCGAAGACTTAGCCAGCTATC
AAGTCATGATCCGCTGTGGAGAAGACATTGCAAAAAATACTGGCTGATATCTGAGGAAGAGAAAACACAG
AAGAATCAGTGTTGGAAATCTCTCTTCATAGATACTTACTCTGATGTAGGAAGATACATTGACCATTATG
CTGCTATTAAAAAGGCCTGGGATGATCTCAAGAAATATTTGGAGCCCAGGTGTCCTCGGATGGTTTTATC
TCTGAAAGAGGGTGCTCGAGAGGAAGACCTCGATGCTGTGGAAGCGCAGATTGGCTGCAAGCTTCCTGAC
GATTATCGATGTTCATACCGAATTCACAATGGACAGAAGTTAGTGGTTCCTGGGTTATTGGGAAGCATGG
CACTGTCTAATCACTATCGTTCTGAAGATTTGTTAGACGTCGATACAGCTGCCGGAGGATTCCAGCAGAG
ACAGGGACTGAAATACTGTCTCCCTTTAACTTTTTGCATACATACTGGTTTGAGTCAGTACATAGCAGTG
GAAGCTGCAGAGGGCCGAAACAAAAATGAAGTTTTCTACCAATGTCCAGACCAAATGGCTCGAAATCCAG
CTGCTATTGACATGTTTATTATAGGTGCTACTTTTACTGACTGGTTTACCTCTTATGTCAAAAATGTTGT
ATCAGGTGGCTTCCCCATCATCAGAGACCAAATTTTCAGATATGTTCACGATCCAGAATGTGTAGCAACA
ACTGGGGATATTACTGTGTCAGTTTCCACATCGTTTCTGCCAGAACTTAGCTCTGTACATCCACCCCACT
ATTTCTTCACATACCGAATCAGGATTGAAATGTCAAAAGATGCACTTCCTGAGAAGGCCTGTCAGTTGGA
CAGTCGCTATTGGAGAATAACAAATGCTAAGGGTGACGTGGAAGAAGTTCAAGGACCTGGAGTAGTTGGT
GAATTTCCAATCATCAGCCCAGGTCGGGTATATGAATACACAAGCTGTACCACATTCTCTACAACATCAG
GATACATGGAAGGATATTATACCTTCCATTTTCTTTACTTTAAAGACAAGATCTTTAATGTTGCCATTCC
CCGATTCCATATGGCATGTCCAACATTCAGGGTGTCTATAGCCCGATTGGTAAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213872 representing NM_033406
Red=Cloning site Green=Tags(s)

MAAMETETAPLTLESLPTDPLLLILSFLDYRDLINCCYVSRRLSQLSSHDPLWRRHCKKYWLISEEEKTQ
KNQCWKSLFIDTYSDVGRYIDHYAAIKKAWDDLKKYLEPRCPRMVLSLKEGAREEDLDAVEAQIGCKLPD
DYRCSYRIHNGQKLVVPGLLGSMALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAV
EAAEGRNKNEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVKNVVSGGFPIIRDQIFRYVHDPECVAT
TGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSKDALPEKACQLDSRYWRITNAKGDVEEVQGPGVVG
EFPIISPGRVYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKIFNVAIPRFHMACPTFRVSIARLVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033406
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033406.3
RefSeq Size 1654 bp
RefSeq ORF 1248 bp
Locus ID 26273
UniProt ID Q9UK99, Q49AF1
Cytogenetics 11p13
Domains DUF525, F-box
Protein Families Druggable Genome
MW 47.3 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. provided by RefSeq, Jul 2008
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Documents
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"NM_033406" in other vectors (4)
SKU Description Size Price
RC213872L3 Lenti-ORF clone of FBXO3 (Myc-DDK-tagged)-Human F-box protein 3 (FBXO3), transcript variant 2 10 ug
$757.00
RC213872L4 Lenti-ORF clone of FBXO3 (mGFP-tagged)-Human F-box protein 3 (FBXO3), transcript variant 2 10 ug
$757.00
RG213872 FBXO3 (tGFP-tagged) - Human F-box protein 3 (FBXO3), transcript variant 2 10 ug
$657.00
SC313363 FBXO3 (untagged)-Human F-box protein 3 (FBXO3), transcript variant 2 10 ug
$503.00

Plasmid Map for RC213872

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213872
Circular map for RC213872

file-alt Citations

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