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NOP2 (NM_001033714) Human Tagged ORF Clone

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SKU
RC213776
NOP2 (Myc-DDK-tagged)-Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $740.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOP2
Synonyms NOL1; NOP120; NSUN1; p120
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213776 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGCAAGTTGGACCCTACGAAGGAGAAGCGGGGGCCAGGCCGAAAGGCCCGGAAGCAGAAGGGTG
CCGAGACAGAACTCGTCAGATTCTTGCCTGCAGTAAGTGACGAAAATTCCAAGAGGCTGTCTAGTCGTGC
TCGAAAGAGGGCAGCCAAGAGGAGATTGGGCTCTGTTGAAGCCCCTAAGACAAATAAGTCTCCTGAGGCC
AAACCATTGCCTGGAAAGCTACCAAAAGGAGCTGTCCAGACAGCTGGTAAGAAGGGACCCCAGTCCCTAT
TTAATGCTCCTCGAGGCAAGAAGCGCCCAGCACCTGGCAGTGATGAGGAAGAGGAGGAGGAAGACTCTGA
AGAAGATGGTATGGTGAACCACGGGGACCTCTGGGGCTCCGAGGACGATGCTGATACGGTAGATGACTAT
GGAGCTGACTCCAACTCTGAGGATGAGGAGGAAGGTGAAGCGTTGCTGCCCATTGAAAGAGCTGCTCGGA
AGCAGAAGGCCCGGGAAGCTGCTGCTGGGATCCAGTGGAGTGAAGAGGAGACCGAGGACGAGGAGGAAGA
GAAAGAAGTGACCCCTGAGTCAGGCCCCCCAAAGGTGGAAGAGGCAGATGGGGGCCTGCAGATCAATGTG
GATGAGGAACCATTTGTGCTGCCCCCTGCTGGGGAGATGGAGCAGGATGCCCAGGCTCCAGACCTGCAAC
GAGTTCACAAGCGGATCCAGGATATTGTGGGAATTCTGCGTGATTTTGGGGCTCAGCGGGAGGAAGGGCG
GTCTCGTTCTGAATACCTGAACCGGCTCAAGAAGGATCTGGCCATTTACTACTCCTATGGAGACTTCCTG
CTTGGCAAGCTCATGGACCTCTTCCCTCTGTCTGAGCTGGTGGAGTTCTTAGAAGCTAATGAGGTGCCTC
GGCCCGTCACCCTCCGGACCAATACCTTGAAAACCCGACGCCGAGACCTTGCACAGGCTCTAATCAATCG
TGGGGTTAACCTGGATCCCCTGGGCAAGTGGTCAAAGACTGGACTAGTGGTGTATGATTCTTCTGTGCCC
ATTGGTGCTACCCCCGAGTACCTGGCTGGGCACTACATGCTGCAGGGAGCCTCCAGCATGTTGCCCGTCA
TGGCCTTGGCACCCCAGGAACATGAGCGGATCCTGGACATGTGTTGTGCCCCTGGAGGAAAGACCAGCTA
CATGGCCCAGCTGATGAAGAACACGGGTGTGATCCTTGCCAATGACGCCAATGCTGAGCGGCTCAAGAGT
GTTGTGGGCAACTTGCATCGGCTGGGAGTCACCAACACCATTATCAGCCACTATGATGGGCGCCAGTTCC
CCAAGGTGGTGGGGGGCTTTGACCGAGTACTGCTGGATGCTCCCTGCAGTGGCACTGGGGTCATCTCCAA
GGATCCAGCCGTGAAGACTAACAAGGATGAGAAGGACATCCTGCGCTGTGCTCACCTCCAGAAGGAGTTG
CTCCTGAGTGCTATTGACTCTGTCAATGCGACCTCCAAGACAGGAGGCTACCTGGTTTACTGCACCTGTT
CTATCACAGTAGAAGAGAATGAGTGGGTGGTAGACTATGCTCTGAAAAAGAGGAATGTGCGACTGGTGCC
CACGGGCCTAGACTTTGGCCAGGAAGGTTTTACCCGCTTTCGAGAAAGGCGCTTCCACCCCAGTCTGCGT
TCTACCCGACGCTTCTACCCTCATACCCACAATATGGATGGGTTCTTCATTGCCAAGTTCAAGAAATTTT
CCAATTCTATCCCTCAGTCCCAGACAGGAAATTCTGAAACAGCCACACCTACAAATGTAGACTTGCCTCA
GGTCATCCCCAAGTCTGAGAACAGCAGCCAGCCAGCCAAGAAAGCCAAGGGGGCTGCAAAGACAAAGCAG
CAGCTGCAGAAACAGCAACATCCCAAGAAGGCCTCCTTCCAGAAGCTGAATGGCATCTCCAAAGGGGCAG
ACTCAGAATTGTCCACTGTACCTTCTGTCACAAAGACCCAAGCTTCCTCCAGCTTCCAGGATAGCAGTCA
GCCAGCTGGAAAAGCCGAAGGGATCAGGGAGCCAAAGGTGACTGGGAAGCTAAAGCAACGATCACCTAAA
TTACAGTCCTCCAAGAAAGTTGCTTTCCTCAGGCAGAATGCCCCTCCCAAGGGCACAGACACACAAACAC
CGGCTGTGTTATCCCCATCCAAGACTCAGGCCACCCTGAAACCTAAGGACCATCATCAGCCCCTTGGAAG
GGCCAAGGGGGTTGAGAAGCAGCAGTTGCCAGAGCAGCCTTTTGAGAAAGCTGCCTTCCAGAAACAGAAT
GATACCCCCAAGGGGCCTCAGCCTCCCACTGTGTCTCCCATCCGTTCCAGCCGCCCCCCACCAGCAAAGA
GGAAGAAATCTCAGTCCAGGGGCAACAGCCAGCTGCTGCTATCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213776 protein sequence
Red=Cloning site Green=Tags(s)

MGRKLDPTKEKRGPGRKARKQKGAETELVRFLPAVSDENSKRLSSRARKRAAKRRLGSVEAPKTNKSPEA
KPLPGKLPKGAVQTAGKKGPQSLFNAPRGKKRPAPGSDEEEEEEDSEEDGMVNHGDLWGSEDDADTVDDY
GADSNSEDEEEGEALLPIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGGLQINV
DEEPFVLPPAGEMEQDAQAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEYLNRLKKDLAIYYSYGDFL
LGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVP
IGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKS
VVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKEL
LLSAIDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPSLR
STRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSSQPAKKAKGAAKTKQ
QLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQPAGKAEGIREPKVTGKLKQRSPK
LQSSKKVAFLRQNAPPKGTDTQTPAVLSPSKTQATLKPKDHHQPLGRAKGVEKQQLPEQPFEKAAFQKQN
DTPKGPQPPTVSPIRSSRPPPAKRKKSQSRGNSQLLLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033714
ORF Size 2424 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033714.3
RefSeq Size 2772 bp
RefSeq ORF 2427 bp
Locus ID 4839
UniProt ID P46087
Cytogenetics 12p13.31
MW 89 kDa
Summary Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033714" in other vectors (6)
SKU Description Size Price
RC213776L1 Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,040.00
RC213776L2 Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2, mGFP tagged 10 ug
$1,040.00
RC213776L3 Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,040.00
RC213776L4 Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2, mGFP tagged 10 ug
$1,040.00
RG213776 NOP2 (tGFP-tagged) - Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2 10 ug
$789.00 $940.00
SC302765 NOP2 (untagged)-Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2 10 ug
$815.00

Plasmid Map for RC213776

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213776
Circular map for RC213776

file-alt Citations

5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader
Yang, Yang, Sun et al
Cell Res. (2017)
AI Summary: OriGene products contributed to this study by providing Myc-Flag-tagged (pCMV6-Entry-ALYREF-Myc-DDK) and GFP-tagged (pCMV6-AC-ALYREF-GFP) human ALYREF plasmids, which were used to investigate the role of ALYREF in mRNA export.
Snippet: Flag-tagged human NSUN1 and NSUN6 plasmids were purchased from Vigene Biosciences, China. Myc-Flag-tagged (pCMV6-Entry-ALYREF-Myc-DDK) and GFP-tagged (pCMV6-AC-ALYREF-GFP) human ALYREF plasmids were purchased from Origene Technologies ... All 3 versions Cite SaveSaving
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