LRP2 (NM_004525) Human Tagged ORF Clone
- TrueORF®
SKU
RC213707
LRP2 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 2 (LRP2)
$5,552.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LRP2 |
| Synonyms | DBS; GP330; LRP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004525 |
| ORF Size | 13965 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq Size | 14392 bp |
| RefSeq ORF | 13968 bp |
| Locus ID | 4036 |
| UniProt ID | P98164, Q7Z5C1, Q7Z5C0 |
| Cytogenetics | 2q31.1 |
| Domains | EGF, EGF_CA, GBP_PSP, ldl_recept_a, ldl_recept_b |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Hedgehog signaling pathway |
| MW | 521.9 kDa |
| Summary | The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).provided by RefSeq, Aug 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Interactions between leucines within the signal peptides of megalin and stanniocalcin-1 are crucial for regulation of mitochondrial metabolism
Li, Holliday, Pan et al
Lab Invest (2022)
Li, Holliday, Pan et al
Lab Invest (2022)
Cited by 11
AI Summary: OriGene’s wild type human LRP2 plasmid (RC213707) was used as the template to generate LRP2 deletion and mutation variants, enabling the study of LRP2-STC1 interactions and their effects on mitochondrial metabolism.
Snippet: mutation variants were generated by cloning or using the Q5 site-directed mutagenesis kit (New England Biolabs E0554S) based on wild type human LRP2 (Origene Save;- STC1 and LRP2 deletion and mutation variants were generated by cloning or using the Q5 site-directed mutagenesis kit (New England Biolabs E0554S) based on wild type human LRP2 (Origene RC213707) or wild type human STC1 cDNA as previously
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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