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CCDC90B (NM_021825) Human Tagged ORF Clone

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SKU
RC213680
CCDC90B (Myc-DDK-tagged)-Human coiled-coil domain containing 90B (CCDC90B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC90B
Synonyms MDS011; MDS025
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213680 representing NM_021825
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAGTCGCCAGGCTTGGCGGCTCTTTCTCTCCCAAGGCAGAGGAGATCGTTGGGTTTCAAGGCCCC
GCGGGCATTTCTCGCCGGCCCTGCGGAGAGAGTTCTTCACTACCACAACCAAGGAGGGATATGATAGGCG
GCCAGTGGATATAACTCCTTTAGAACAAAGGAAATTAACTTTTGATACCCATGCATTGGTTCAGGACTTG
GAAACTCATGGATTTGACAAAACACAAGCAGAAACAATTGTATCAGCGTTAACTGCTTTATCAAATGTCA
GCCTGGATACTATCTATAAAGAGATGGTCACTCAAGCTCAACAGGAAATAACAGTACAACAGCTAATGGC
TCATTTGGATGCTATCAGGAAAGACATGGTCATCCTAGAGAAAAGTGAATTTGCAAATCTGAGAGCAGAG
AATGAGAAAATGAAAATTGAATTAGACCAAGTTAAGCAACAACTAATGCATGAAACCAGTCGAATCAGAG
CAGATAATAAACTGGATATCAACTTAGAAAGGAGCAGAGTAACAGATATGTTTACAGATCAAGAAAAGCA
ACTTATGGAAACAACTACAGAATTTACAAAAAAGGATACTCAAACCAAAAGTATTATTTCAGAGACCAGT
AATAAAATTGACGCTGAAATTGCTTCCTTAAAAACACTGATGGAATCTAACAAACTTGAGACAATTCGTT
ATCTTGCAGCTTCAGTGTTTACTTGCCTGGCAATAGCATTGGGATTTTATAGATTCTGGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213680 representing NM_021825
Red=Cloning site Green=Tags(s)

MNSRQAWRLFLSQGRGDRWVSRPRGHFSPALRREFFTTTTKEGYDRRPVDITPLEQRKLTFDTHALVQDL
ETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQLMAHLDAIRKDMVILEKSEFANLRAE
NEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDMFTDQEKQLMETTTEFTKKDTQTKSIISETS
NKIDAEIASLKTLMESNKLETIRYLAASVFTCLAIALGFYRFWK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021825
ORF Size 762 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021825.3, NP_068597.2
RefSeq Size 1585 bp
RefSeq ORF 765 bp
Locus ID 60492
UniProt ID Q9GZT6
Cytogenetics 11q14.1
Protein Families Transmembrane
MW 29.3 kDa
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"NM_021825" in other vectors (6)
SKU Description Size Price
RC213680L1 Lenti ORF clone of Human coiled-coil domain containing 90B (CCDC90B), Myc-DDK-tagged 10 ug
$600.00
RC213680L2 Lenti ORF clone of Human coiled-coil domain containing 90B (CCDC90B), mGFP tagged 10 ug
$600.00
RC213680L3 Lenti ORF clone of Human coiled-coil domain containing 90B (CCDC90B), Myc-DDK-tagged 10 ug
$600.00
RC213680L4 Lenti ORF clone of Human coiled-coil domain containing 90B (CCDC90B), mGFP tagged 10 ug
$600.00
RG213680 CCDC90B (tGFP-tagged) - Human coiled-coil domain containing 90B (CCDC90B) 10 ug
$500.00
SC310596 CCDC90B (untagged)-Human coiled-coil domain containing 90B (CCDC90B) 10 ug
$330.00

Plasmid Map for RC213680

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213680
Circular map for RC213680

file-alt Citations

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