SSR2 (NM_003145) Human Tagged ORF Clone
SKU
RC213580
SSR2 (Myc-DDK-tagged)-Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2)
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SSR2 |
| Synonyms | HSD25; TLAP; TRAP-BETA; TRAPB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003145 |
| ORF Size | 549 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003145.4 |
| RefSeq Size | 1093 bp |
| RefSeq ORF | 552 bp |
| Locus ID | 6746 |
| UniProt ID | P43308 |
| Cytogenetics | 1q22 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 20 kDa |
| Summary | The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein (alpha-SSR or SSR1) and a 22-kD glycoprotein (beta-SSR or SSR2). The human beta-signal sequence receptor gene (SSR2) maps to chromosome bands 1q21-q23. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003145" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213580L1 | Lenti ORF clone of Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC213580L2 | Lenti ORF clone of Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2), mGFP tagged | 10 ug |
$600.00
|
|
| RC213580L3 | Lenti ORF clone of Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC213580L4 | Lenti ORF clone of Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2), mGFP tagged | 10 ug |
$600.00
|
|
| RG213580 | SSR2 (tGFP-tagged) - Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2) | 10 ug |
$500.00
|
|
| SC118190 | SSR2 (untagged)-Human signal sequence receptor, beta (translocon-associated protein beta) (SSR2) | 10 ug |
$300.00
|
|
Citations
Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import
Nguyen, Stutz, Schorr et al
Nat Commun (2018) 9 (1), 3765 DOI: 10.1038/s41467-018-06188-z
Nguyen, Stutz, Schorr et al
Nat Commun (2018) 9 (1), 3765 DOI: 10.1038/s41467-018-06188-z
AI Summary: OriGene contributed to this study by providing the murine monoclonal anti-mycDDK-tag antibody (TA50011), which was used for detecting tagged proteins in validation experiments such as western blotting.
Snippet: … We purchased murine monoclonal antibodies against β-actin (Sigma, A5441, used dilution 1:10,000), Ost48 (Santa Cruz Biotechnology, sc-74408, used dilution 1:1,000), and the mycDDK-tag (Origene, TA50011, used dilution 1:1,000), and rabbit antibodies against ACP2 (Thermo Fisher Scientific, PA5-29961, used dilution 1:500), PPIC (Abcam, ab184552, used dilution 1:1,000), TMED5 (Sigma-Ald;... … We purchased murine monoclonal antibodies against β-actin (Sigma, A5441, used dilution 1:10,000), Ost48 (Santa Cruz Biotechnology, sc-74408, used dilution 1:1,000), and the mycDDK-tag (Origene, TA50011, used dilution 1:1,000), and rabbit antibodies against ACP2 (Thermo Fisher Scientific, PA5-29961, used dilution 1:500), PPIC (Abcam, ab184552, used dilution 1:1,000), TMED5 (Sigma-Ald
Showing 1-1 of 1 papers.
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