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SLC14A2 (NM_007163) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213567
SLC14A2 (Myc-DDK-tagged)-Human solute carrier family 14 (urea transporter), member 2 (SLC14A2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $842.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC14A2
Synonyms hUT-A6; HUT2; UT-A2; UT2; UTA; UTR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213567 representing NM_007163
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGACCCCCACAGCAGTCCTCTCCTGCCAGAGCCACTTTCCAGCAGATACAAACTTTACGAGGCAG
AGTTTACCAGCCCGAGCTGGCCCTCGACATCCCCGGATACTCACCCAGCTCTGCCCCTCCTGGAAATGCC
TGAAGAAAAGGATCTCCGGTCTTCCAATGAAGACAGTCACATTGTGAAGATCGAAAAGCTCAATGAAAGG
AGTAAAAGGAAAGACGACGGGGTGGCCCATCGGGACTCAGCAGGCCAAAGGTGCATCTGCCTCTCCAAAG
CAGTGGGCTACCTCACGGGCGACATGAAGGAGTACAGGATCTGGCTGAAAGACAAGCACCTTGCCCTCCA
GTTCATAGACTGGGTCCTGAGAGGGACCGCTCAGGTGATGTTCGTCAACAATCCTCTCAGCGGCCTCATC
ATCTTCATAGGGCTGCTGATCCAGAATCCCTGGTGGACAATCACTGGGGGCCTGGGGACAGTGGTCTCGA
CCTTAACAGCTCTCGCCTTGGGCCAAGACAGGTCTGCCATTGCCTCAGGACTCCATGGGTACAACGGGAT
GCTGGTGGGACTGCTGATGGCCGCGTTCTCGGAGAAGTTAGACTACTACTGGTGGCTTCTGTTTCCTGTG
ACCTTCACAGCCATGTCCTGCCCAGTTCTTTCTAGTGCCTTGAATTCCATCTTCAGCAAGTGGGACCTCC
CGGTCTTCACTCTGCCCTTCAACATTGCAGTCACCTTGTACCTTGCAGCCACAGGCCACTACAACCTCTT
CTTCCCCACAACACTGGTAGAGCCTGTGTCTTCAGTGCCCAATATCACCTGGACAGAGATGGAAATGCCC
CTGCTGTTACAAGCCATCCCTGTTGGGGTCGGCCAGGTGTATGGCTGTGACAATCCCTGGACAGGCGGCG
TGTTCCTGGTGGCTCTGTTCATCTCCTCGCCACTCATCTGCTTGCATGCAGCCATTGGCTCAATCGTGGG
GCTGCTAGCAGCCCTGTCAGTGGCCACACCCTTCGAGACCATCTACACAGGCCTCTGGAGCTACAACTGC
GTCCTCTCCTGCATCGCCATCGGAGGCATGTTCTATGCCCTCACCTGGCAGACTCACCTGCTGGCCCTCA
TCTGTGCCCTGTTCTGTGCATACATGGAAGCAGCCATCTCCAACATCATGTCAGTGGTGGGCGTGCCACC
AGGCACCTGGGCCTTCTGCCTTGCCACCATCATCTTCCTGCTCCTGACGACAAACAACCCAGCCATCTTC
AGACTCCCACTCAGCAAAGTCACCTACCCCGAGGCCAACCGCATCTACTACCTGACAGTGAAAAGCGGTG
AAGAAGAGAAGGCCCCCAGCGGAGGCGGTGGGGAGCATCCACCCACAGCAGGCCCAAAGGTGGAGGAGGG
CTCGGAGGCTGTGCTCTCCAAGCACAGGAGTGTATTTCACATCGAGTGGTCATCCATTCGGAGGAGGAGC
AAAGTGTTTGGAAAAGGCGAACACCAGGAAAGACAAAACAAAGACCCATTTCCCTATCAATACCGGAAGC
CCACAGTCGAGCTGCTTGATCTGGACACCATGGAGGAGAGCTCTGAGATAAAAGTGGAAACAAACATTTC
CAAGACATCCTGGATTCGGAGTTCCATGGCTGCCAGTGGGAAAAGGGTGAGCAAAGCCCTCAGCTACATC
ACAGGAGAGATGAAGGAGTGTGGAGAGGGACTTAAAGACAAGTCCCCAGTGTTCCAGTTCTTTGACTGGG
TCCTCCGAGGCACATCTCAAGTGATGTTTGTGAACAACCCCCTCAGCGGCATCCTCATCATCCTCGGCCT
CTTCATCCAGAACCCCTGGTGGGCGATCTCAGGCTGCCTGGGTACCATCATGTCCACCTTGACAGCCCTC
ATCCTGAGTCAGGACAAGTCGGCCATCGCTGCAGGATTTCACGGCTACAATGGGGTGCTGGTGGGGCTGC
TGATGGCCGTGTTCTCAGACAAAGGTGACTACTACTGGTGGCTGTTGCTACCCGTCATCATCATGTCCAT
GTCTTGCCCCATCCTCTCCAGTGCCCTGGGTACCATCTTCAGCAAGTGGGACCTCCCAGTCTTCACACTG
CCCTTCAATATCACTGTGACTTTGTACCTGGCAGCCACGGGCCACTACAACCTTTTCTTCCCCACAGCGC
TGCTGCAGCCTGCATCCGCCATGCCCAACATCACCTGGTCAGAGGTCCAAGTGCCCTTGCTTTTGAGAGC
CATCCCCGTTGGAATTGGCCAAGTGTACGGCTGTGATAACCCCTGGACTGGAGGCATCTTCCTCATAGCT
CTGTTCATATCCTCACCTCTCATTTGCTTGCATGCAGCAATTGGATCCACCATGGGGATGCTAGCAGCAC
TCACTATTGCGACGCCCTTTGACTCCATCTACTTCGGCCTGTGTGGCTTCAACAGCACCCTCGCATGCAT
AGCGATAGGAGGCATGTTCTACGTCATCACCTGGCAGACGCACCTCCTCGCCATCGCCTGCGCACTGTTT
GCTGCCTACCTGGGTGCTGCCCTGGCTAACATGTTATCTGTGTTTGGATTGCCGCCCTGCACTTGGCCCT
TCTGTCTCTCAGCTCTCACCTTCCTGCTCCTGACGACCAATAACCCCGCCATCTACAAGCTCCCGCTCAG
CAAAGTCACCTACCCAGAGGCCAACCGCATCTACTACCTGTCCCAGGAGAGAAACAGAAGGGCATCAATC
ATAACAAAGTATCAGGCCTACGATGTCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213567 representing NM_007163
Red=Cloning site Green=Tags(s)

MSDPHSSPLLPEPLSSRYKLYEAEFTSPSWPSTSPDTHPALPLLEMPEEKDLRSSNEDSHIVKIEKLNER
SKRKDDGVAHRDSAGQRCICLSKAVGYLTGDMKEYRIWLKDKHLALQFIDWVLRGTAQVMFVNNPLSGLI
IFIGLLIQNPWWTITGGLGTVVSTLTALALGQDRSAIASGLHGYNGMLVGLLMAAFSEKLDYYWWLLFPV
TFTAMSCPVLSSALNSIFSKWDLPVFTLPFNIAVTLYLAATGHYNLFFPTTLVEPVSSVPNITWTEMEMP
LLLQAIPVGVGQVYGCDNPWTGGVFLVALFISSPLICLHAAIGSIVGLLAALSVATPFETIYTGLWSYNC
VLSCIAIGGMFYALTWQTHLLALICALFCAYMEAAISNIMSVVGVPPGTWAFCLATIIFLLLTTNNPAIF
RLPLSKVTYPEANRIYYLTVKSGEEEKAPSGGGGEHPPTAGPKVEEGSEAVLSKHRSVFHIEWSSIRRRS
KVFGKGEHQERQNKDPFPYQYRKPTVELLDLDTMEESSEIKVETNISKTSWIRSSMAASGKRVSKALSYI
TGEMKECGEGLKDKSPVFQFFDWVLRGTSQVMFVNNPLSGILIILGLFIQNPWWAISGCLGTIMSTLTAL
ILSQDKSAIAAGFHGYNGVLVGLLMAVFSDKGDYYWWLLLPVIIMSMSCPILSSALGTIFSKWDLPVFTL
PFNITVTLYLAATGHYNLFFPTALLQPASAMPNITWSEVQVPLLLRAIPVGIGQVYGCDNPWTGGIFLIA
LFISSPLICLHAAIGSTMGMLAALTIATPFDSIYFGLCGFNSTLACIAIGGMFYVITWQTHLLAIACALF
AAYLGAALANMLSVFGLPPCTWPFCLSALTFLLLTTNNPAIYKLPLSKVTYPEANRIYYLSQERNRRASI
ITKYQAYDVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007163
ORF Size 2760 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007163.2, NP_009094.2
RefSeq Size 4166 bp
RefSeq ORF 2763 bp
Locus ID 8170
UniProt ID Q15849
Cytogenetics 18q12.3
Domains UT
Protein Families Transmembrane
MW 101 kDa
Summary The protein encoded by this gene belongs to the urea transporter family. In mammalian cells, urea is the chief end product of nitrogen catabolism, and plays an important role in the urinary concentration mechanism. This protein is expressed in the inner medulla of the kidney, and mediates rapid transepithelial urea transport across the inner medullary collecting duct. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_007163" in other vectors (4)
SKU Description Size Price
RC213567L3 Lenti ORF clone of Human solute carrier family 14 (urea transporter), member 2 (SLC14A2), Myc-DDK-tagged 10 ug
$1,142.00
RC213567L4 Lenti ORF clone of Human solute carrier family 14 (urea transporter), member 2 (SLC14A2), mGFP tagged 10 ug
$1,142.00
RG213567 SLC14A2 (tGFP-tagged) - Human solute carrier family 14 (urea transporter), member 2 (SLC14A2) 10 ug
$789.00 $1,042.00
SC310046 SLC14A2 (untagged)-Human solute carrier family 14 (urea transporter), member 2 (SLC14A2) 10 ug
$843.00

Plasmid Map for RC213567

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213567
Circular map for RC213567

file-alt Citations

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