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PACE4 (PCSK6) (NM_138320) Human Tagged ORF Clone

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SKU
RC213553
PCSK6 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $893.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PACE4
Synonyms PACE4; SPC4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213553 representing NM_138320
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCGCGCGCGCCGCCTGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGCCGCCACCGACACCG
CCGCGGGCGCGGGGGGCGCGGGGGGCGCGGGGGGCGCCGGCGGGCCCGGGTTCCGGCCGCTCGCGCCGCG
TCCCTGGCGCTGGCTGCTGCTGCTGGCGCTGCCTGCCGCCTGCTCCGCGCCCCCGCCGCGCCCCGTCTAC
ACCAACCACTGGGCGGTGCAAGTGCTGGGCGGCCCGGCCGAGGCGGACCGCGTGGCGGCGGCGCACGGGT
ACCTCAACTTGGGCCAGATTGGAAACCTGGAAGATTACTACCATTTTTATCACAGCAAAACCTTTAAAAG
ATCAACCTTGAGTAGCAGAGGCCCTCACACCTTCCTCAGAATGGACCCCCAGGTGAAATGGCTCCAGCAA
CAGGAAGTGAAACGAAGGGTGAAGAGACAGGTGCGAAGTGACCCGCAGGCCCTTTACTTCAACGACCCCA
TTTGGTCCAACATGTGGTACCTGCATTGTGGCGACAAGAACAGTCGCTGCCGGTCGGAAATGAATGTCCA
GGCAGCGTGGAAGAGGGGCTACACAGGAAAAAACGTGGTGGTCACCATCCTTGATGATGGCATAGAGAGA
AATCACCCTGACCTGGCCCCAAATTATGATTCCTACGCCAGCTACGACGTGAACGGCAATGATTATGACC
CATCTCCACGATATGATGCCAGCAATGAAAATAAACACGGCACTCGTTGTGCGGGAGAAGTTGCTGCTTC
AGCAAACAATTCCTACTGCATCGTGGGCATAGCGTACAATGCCAAAATAGGAGGCATCCGCATGCTGGAC
GGCGATGTCACAGATGTGGTCGAGGCAAAGTCGCTGGGCATCAGACCCAACTACATCGACATTTACAGTG
CCAGCTGGGGGCCGGACGACGACGGCAAGACGGTGGACGGGCCCGGCCGACTGGCTAAGCAGGCTTTCGA
GTATGGCATTAAAAAGGGCCGGCAGGGCCTGGGCTCCATTTTCGTCTGGGCATCTGGGAATGGCGGGAGA
GAGGGGGACTACTGCTCGTGCGATGGCTACACCAACAGCATCTACACCATCTCCGTCAGCAGCGCCACCG
AGAATGGCTACAAGCCCTGGTACCTGGAAGAGTGTGCCTCCACCCTGGCCACCACCTACAGCAGTGGGGC
CTTTTATGAGCGAAAAATCGTCACCACGGATCTGCGTCAGCGCTGTACCGATGGCCACACTGGGACCTCA
GTCTCTGCCCCCATGGTGGCGGGCATCATCGCCTTGGCTCTAGAAGCAAACAGCCAGTTAACCTGGAGGG
ACGTCCAGCACCTGCTAGTGAAGACATCCCGGCCGGCCCACCTGAAAGCGAGCGACTGGAAAGTGAACGG
CGCGGGTCATAAAGTTAGCCATTTCTATGGATTTGGTTTGGTGGACGCAGAAGCTCTCGTTGTGGAGGCA
AAGAAGTGGACAGCAGTGCCATCGCAGCACATGTGTGTGGCCGCCTCGGACAAGAGACCCAGGAGCATCC
CCTTAGTGCAGGTGCTGCGGACTACGGCCCTGACCAGCGCCTGCGCGGAGCACTCGGACCAGCGGGTGGT
CTACTTGGAGCACGTGGTGGTTCGCACCTCCATCTCACACCCACGCCGAGGAGACCTCCAGATCTACCTG
GTTTCTCCCTCGGGAACCAAGTCTCAACTTCTGGCAAAGAGGTTGCTGGATCTTTCCAATGAAGGGTTTA
CAAACTGGGAATTCATGACTGTCCACTGCTGGGGAGAAAAGGCTGAAGGGCAGTGGACCTTGGAAATCCA
AGATCTGCCATCCCAGGTCCGCAACCCGGAGAAGCAAGGGAAGTTGAAAGAATGGAGCCTCATACTGTAT
GGCACAGCAGAGCACCCGTACCACACCTTCAGTGCCCATCAGTCCCGCTCGCGGATGCTGGAGCTCTCAG
CCCCAGAGCTGGAGCCACCCAAGGCTGCCCTGTCACCCTCCCAGGTGGAAGTTCCTGAAGATGAGGAAGA
TTACACAGCTCAATCCACCCCAGGCTCTGCTAATATTTTACAGACCAGTGTGTGCCATCCGGAGTGTGGT
GACAAAGGCTGTGATGGCCCCAATGCAGACCAGTGCTTGAACTGCGTCCACTTCAGCCTGGGGAGTGTCA
AGACCAGCAGGAAGTGCGTGAGTGTGTGCCCCTTGGGCTACTTTGGGGACACAGCAGCAAGACGCTGTCG
CCGGTGCCACAAGGGGTGTGAGACCTGCTCCAGCAGAGCTGCGACGCAGTGCCTGTCTTGCCGCCGCGGG
TTCTATCACCACCAGGAGATGAACACCTGTGTGACCCTCTGTCCTGCAGGATTTTATGCTGATGAAAGTC
AGAAAAATTGCCTTAAATGCCACCCAAGCTGTAAAAAGTGCGTGGATGAACCTGAGAAATGTACTGTCTG
TAAAGAAGGATTCAGCCTTGCACGGGGCAGCTGCATTCCTGACTGTGAGCCAGGCACCTACTTTGACTCA
GAGCTGATCAGATGTGGGGAATGCCATCACACCTGCGGAACCTGCGTGGGGCCAGGCAGAGAAGAGTGCA
TTCACTGTGCGAAAAACTTCCACTTCCACGACTGGAAGTGTGTGCCAGCCTGTGGTGAGGGCTTCTACCC
AGAAGAGATGCCGGGCTTGCCCCACAAAGTGTGTCGAAGGTACGGTCCTCCTGGCGGGGAACGGCAGGCA
GCTGTGTCCAGCAAAGGAGTGCCTGGAGGGCAGAGTCTGGCAGCATCTTCCCCAGGGGCCGGGGAGGGCA
TGTTGCATCACCCCACTGTGGACAGGAGCCCATTTACAGAGCTGCTGAGGGGCCTTCGTCCCTTTGTTCA
CTGGATGCATATTTGTTGGGTCCCTGCTGTGGGCAGACACCGTGCAGCAGCGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213553 representing NM_138320
Red=Cloning site Green=Tags(s)

MPPRAPPAPGPRPPPRAAAATDTAAGAGGAGGAGGAGGPGFRPLAPRPWRWLLLLALPAACSAPPPRPVY
TNHWAVQVLGGPAEADRVAAAHGYLNLGQIGNLEDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQ
QEVKRRVKRQVRSDPQALYFNDPIWSNMWYLHCGDKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIER
NHPDLAPNYDSYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLD
GDVTDVVEAKSLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGR
EGDYCSCDGYTNSIYTISVSSATENGYKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTS
VSAPMVAGIIALALEANSQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHFYGFGLVDAEALVVEA
KKWTAVPSQHMCVAASDKRPRSIPLVQVLRTTALTSACAEHSDQRVVYLEHVVVRTSISHPRRGDLQIYL
VSPSGTKSQLLAKRLLDLSNEGFTNWEFMTVHCWGEKAEGQWTLEIQDLPSQVRNPEKQGKLKEWSLILY
GTAEHPYHTFSAHQSRSRMLELSAPELEPPKAALSPSQVEVPEDEEDYTAQSTPGSANILQTSVCHPECG
DKGCDGPNADQCLNCVHFSLGSVKTSRKCVSVCPLGYFGDTAARRCRRCHKGCETCSSRAATQCLSCRRG
FYHHQEMNTCVTLCPAGFYADESQKNCLKCHPSCKKCVDEPEKCTVCKEGFSLARGSCIPDCEPGTYFDS
ELIRCGECHHTCGTCVGPGREECIHCAKNFHFHDWKCVPACGEGFYPEEMPGLPHKVCRRYGPPGGERQA
AVSSKGVPGGQSLAASSPGAGEGMLHHPTVDRSPFTELLRGLRPFVHWMHICWVPAVGRHRAAAG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138320
ORF Size 2925 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138320.1, NP_612193.1
RefSeq Size 3372 bp
RefSeq ORF 2927 bp
Locus ID 5046
Cytogenetics 15q26.3
Protein Families Druggable Genome, Protease, Secreted Protein
MW 100.6 kDa
Summary This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Feb 2014
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Documents
Resources
"NM_138320" in other vectors (4)
SKU Description Size Price
RC213553L3 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7, Myc-DDK-tagged 10 ug
$1,193.00
RC213553L4 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7, mGFP tagged 10 ug
$1,193.00
RG213553 PCSK6 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7 10 ug
$1,093.00
SC309533 PCSK6 (untagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7 10 ug
$983.00

Plasmid Map for RC213553

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213553
Circular map for RC213553

file-alt Citations

Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7
Sathyamurthy, Kim, Bang et al
Biotechnol. Bioeng. (2014)
Cited by 11
AI Summary: OriGene contributed to this study by providing the human PACE4 cDNA in the pCMV6 vector, which was used to transiently express PACE4 in CHO-BMP-7 cells for evaluating its role in BMP-7 processing.
Snippet: (NM_004716.2) were amplified by PCR from the HEK293E cDNA pool using gene-specific primers. Human PACE4 cDNA (NM_002570.3) in pCMV6 vector was obtained from Origene (Rockville, MD). The secreted forms (PACEsol ... Cite SaveSaving
Showing 1-1 of 1 papers.
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