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KLHL15 (NM_030624) Human Tagged ORF Clone

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SKU
RC213520
KLHL15 (Myc-DDK-tagged)-Human kelch-like 15 (Drosophila) (KLHL15)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $563.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHL15
Synonyms HEL-S-305
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213520 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCATCCACGACACCAGTGTCTCTGCTGGGTTCAGAGCAC
TGTATGAGGAGGGATTGCTTCTTGATGTCACTCTGGTTATTGAAGATCATCAGTTCCAGGCCCATAAAGC
ACTCTTGGCCACCCAGAGTGATTACTTCAGAATTATGTTTACTGCAGACATGAGGGAACGAGATCAGGAC
AAAATTCATTTAAAAGGTCTAACAGCTACCGGTTTCAGCCATGTCCTGCAATTTATGTACTATGGAACTA
TAGAGCTGAGTATGAATACCGTTCATGAGATTCTTCAGGCTGCCATGTATGTTCAACTTATAGAAGTGGT
GAAGTTCTGCTGCTCTTTTCTCTTAGCGAAGATCTGCCTAGAAAATTGTGCAGAAATTATGAGACTCTTA
GATGATTTCGGCGTAAACATCGAGGGAGTCAGGGAGAAGTTAGACACCTTTCTGCTAGACAACTTTGTGC
CACTCATGTCTAGGCCTGACTTTCTGTCCTATCTGAGCTTTGAGAAGCTCATGTCTTACTTGGATAATGA
TCATCTGAGCAGGTTCCCAGAGATAGAGCTGTACGAGGCTGTGCAGTCTTGGCTGCGGCATGATAGAAGA
CGCTGGAGACATACCGATACCATCATTCAGAATATCCGGTTTTGCTTGATGACCCCAACCAGCGTTTTTG
AGAAGGTTAAGACATCAGAATTTTATAGATACTCCCGACAGCTCCGTTACGAAGTTGACCAAGCATTGAA
TTACTTTCAGAATGTTCACCAGCAGCCTTTGTTGGATATGAAGTCAAGCCGCATCCGTTCTGCAAAACCG
CAAACTACAGTATTTCGAGGAATGATTGGACATAGCATGGTTAACAGTAAAATACTTCTCTTAAAGAAAC
CAAGAGTCTGGTGGGAGCTAGAAGGCCCACAAGTACCTCTGCGACCTGACTGCCTTGCTATCGTCAATAA
TTTTGTGTTCCTGTTAGGCGGGGAAGAGCTGGGCCCGGATGGTGAATTCCATGCTTCTTCCAAAGTATTC
AGGTATGACCCGAGACAGAACTCCTGGCTGCAGATGGCAGATATGTCTGTACCACGCTCTGAATTTGCTG
TAGGTGTTATTGGGAAGTTTATTTACGCCGTAGCAGGCAGAACCAGAGATGAGACTTTCTATTCAACTGA
GAGATATGACATCACCAACGATAAATGGGAATTTGTGGATCCTTATCCAGTTAACAAATATGGACATGAG
GGGACAGTGCTCAATAACAAATTGTTTATCACCGGTGGAATCACCTCATCTTCCACCTCCAAGCAAGTGT
GCGTGTTTGACCCCAGCAAAGAAGGGACCATAGAACAACGGACCAGGAGAACTCAAGTGGTTACCAACTG
TTGGGAGAATAAGAGCAAGATGAATTACGCGAGATGCTTTCACAAGATGATTTCTTACAATGGCAAGCTT
TATGTCTTCGGTGGTGTCTGTGTGATCTTGAGGGCCTCTTTCGAATCTCAGGGATGCCCTTCTACAGAAG
TATACAACCCAGAGACTGATCAGTGGACCATCTTGGCATCCATGCCGATTGGTAGAAGTGGCCATGGTGT
GACTGTGCTGGACAAACAAATAATGGTTCTTGGAGGCCTTTGTTATAATGGTCATTACAGCGATTCCATC
CTCACTTTTGATCCGGATGAAAACAAGTGGAAGGAAGATGAGTACCCTCGGATGCCCTGCAAGCTGGATG
GTTTACAAGTATGCAACCTGCATTTTCCGGACTATGTACTGGATGAGGTCAGGCGTTGCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213520 protein sequence
Red=Cloning site Green=Tags(s)

MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQD
KIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLL
DDFGVNIEGVREKLDTFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRR
RWRHTDTIIQNIRFCLMTPTSVFEKVKTSEFYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKP
QTTVFRGMIGHSMVNSKILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVF
RYDPRQNSWLQMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHE
GTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCFHKMISYNGKL
YVFGGVCVILRASFESQGCPSTEVYNPETDQWTILASMPIGRSGHGVTVLDKQIMVLGGLCYNGHYSDSI
LTFDPDENKWKEDEYPRMPCKLDGLQVCNLHFPDYVLDEVRRCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_030624
ORF Size 1812 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030624.3
RefSeq Size 6290 bp
RefSeq ORF 1815 bp
Locus ID 80311
UniProt ID Q96M94
Cytogenetics Xp22.11
MW 69.8 kDa
Summary This gene encodes a member of the kelch-like family of proteins that share a common domain structure consisting of an N-terminal broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger domain and C-terminal kelch repeat motifs. The encoded protein may be involved in protein ubiquitination and cytoskeletal organization. provided by RefSeq, Apr 2009
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"NM_030624" in other vectors (6)
SKU Description Size Price
RC213520L1 Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), Myc-DDK-tagged 10 ug
$863.00
RC213520L2 Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), mGFP tagged 10 ug
$863.00
RC213520L3 Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), Myc-DDK-tagged 10 ug
$863.00
RC213520L4 Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), mGFP tagged 10 ug
$863.00
RG213520 KLHL15 (tGFP-tagged) - Human kelch-like 15 (Drosophila) (KLHL15) 10 ug
$763.00
SC305271 KLHL15 (untagged)-Human kelch-like 15 (Drosophila) (KLHL15) 10 ug
$619.00

file-alt Citations

Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection
Ferretti, Himmels, Trenner et al
Nat Commun (2016) 7, 12628 DOI: 10.1038/ncomms12628
Cited by 67
AI Summary: OriGene products contributed to this study by providing the pCMV6-myc-FLAG-KLHL15 expression plasmid and Trisilencer-27 human KLHL15 siRNA B, which were used to manipulate KLHL15 levels and investigate its role in CtIP protein turnover and DNA-end resection.
Snippet: The pCMV6-myc-FLAG-KLHL15 expression plasmid was obtained from Origene (NM_030624 Human cDNA True ORF Clone, Origene). Trisilencer-27 human KLHL15 siRNA B targeting the 30 -untranslated region of KLHL15 (KLHL15#3) was purchased from OriGene
Showing 1-1 of 1 papers.
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