NKAPD1 (NM_018195) Human Tagged ORF Clone

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SKU
RC213479
C11orf57 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NKAPD1
Synonyms C11orf57
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213479 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGGATTCCACTGGGGAAGGTCCTCCTGAGGAATGTCATCCGGCACACAGATGCTCACAATAAGA
TTCAGGAGGAATCAGATATGTGGGAAATAAGAGAACTGGAAAAACAGATGGAAGATGCTTACCGGGGGAC
CAAAAGGAAAATGCTACCCAGCAGTTCAAGCCGGATGCGCAGTGATGGTTTTGATGAAGAAAGTCAAAGA
TACTATTGGAGGCCAAAGAATGAAATTTCTGGGACACTGGAAGATGATTTTCTTAAGGCTAAATCCTGGA
ATAAAAAGTTCTATGATTATGAAGCAAACATGCCAGACAGATGGGGTCACAGTGGTTATAAAGAGTTATA
CCCTGAAGAATTTGAAACAGACAGTAGTGATCAGCAAGATATTACCAACGGGAAGAAAACATCTCCCCAG
GTAAAGTCATCTACCCATGAATCCCGCAAACACAAGAAGTCAAAGAAATCCCACAAAAAAAAGCAGAAAA
AAAGGTCACACAAAAAACAGAAGAAAAGCAAAAAGGAAGCCACAGATATAACAGCAGATTCCTCGAGTGA
GTTCTCAGAAGAAACTGGGGCTTCTGGTACAAGGAAAGGGAAACAACCACATAAACGCAAGAAAAAATCC
AGGAAAAAGTCTCTCAAAAAACCTGCTTTATTCTTAGAGGCAGAAAGTAACACTTCACATTCAGATGATT
CAGCATCCAGCAGTTCTGAGGAAAGTGAGGAAAGAGACACTAAGAAAACCAAAAGGAAAAAGAGAGAGAA
AAAAGCCCATACCTCTGTAGCCAACAATGAAATACAGGAGAGGACAAACAAACGCACAAATTGGAAAGTA
GCTACAGATGAAAGGTCTGCTGAGAGCTCAGAGGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213479 protein sequence
Red=Cloning site Green=Tags(s)

MSRIPLGKVLLRNVIRHTDAHNKIQEESDMWEIRELEKQMEDAYRGTKRKMLPSSSSRMRSDGFDEESQR
YYWRPKNEISGTLEDDFLKAKSWNKKFYDYEANMPDRWGHSGYKELYPEEFETDSSDQQDITNGKKTSPQ
VKSSTHESRKHKKSKKSHKKKQKKRSHKKQKKSKKEATDITADSSSEFSEETGASGTRKGKQPHKRKKKS
RKKSLKKPALFLEAESNTSHSDDSASSSSEESEERDTKKTKRKKREKKAHTSVANNEIQERTNKRTNWKV
ATDERSAESSEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018195
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018195.2, NP_060665.2
RefSeq Size 3757 bp
RefSeq ORF 882 bp
Locus ID 55216
UniProt ID A0A024R3H5
Cytogenetics 11q23.1
MW 34.2 kDa
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Documents
Resources
"NM_018195" in other vectors (5)
SKU Description Size Price
RC213479L3 Lenti ORF clone of Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC213479L4 Lenti ORF clone of Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1, mGFP tagged 10 ug
$600.00
RG213479 C11orf57 (tGFP-tagged) - Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1 10 ug
$489.00 $500.00
SC108687 C11orf57 (untagged)-Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1 10 ug
$300.00
SC317429 C11orf57 (untagged)-Human chromosome 11 open reading frame 57 (C11orf57), transcript variant 1 10 ug
$330.00

Plasmid Map for RC213479

Download sequence for

Cloning scheme for RC213479

Circular map for RC213479

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