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PHF20L1 (NM_032205) Human Tagged ORF Clone

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SKU
RC213315
PHF20L1 (Myc-DDK-tagged)-Human PHD finger protein 20-like 1 (PHF20L1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $533.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHF20L1
Synonyms CGI-72; TDRD20B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213315 representing NM_032205
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAAAAAGCCCCCAAATCGCCCTGGAATCACTTTTGAGATTGGTGCTCGTTTGGAGGCACTGGACT
ACTTACAAAAATGGTATCCATCACGAATTGAAAAAATTGACTATGAGGAGGGCAAGATGTTGGTCCATTT
TGAGCGCTGGAGTCATCGTTATGATGAGTGGATTTACTGGGATAGCAATAGATTGCGACCCCTTGAGAGA
CCAGCACTAAGAAAAGAAGGGCTAAAAGATGAGGAAGATTTCTTTGATTTTAAAGCTGGAGAAGAAGTTC
TGGCTCGTTGGACAGACTGTCGCTATTACCCTGCCAAGATTGAAGCAATTAACAAAGAAGGAACATTTAC
AGTTCAGTTTTATGATGGAGTAATTCGTTGTTTAAAAAGAATGCACATTAAAGCCATGCCCGAGGATGCT
AAGGGGCAGGTGAAATCCCAGCATCCACTAAGCTGGTGTTGTCCTATCGACCCAGCTGGATCGTGTAACC
AGTCTATGGGAAGTGAGGATTGGATAGCTTTAGTCAAAGCAGCTGCTGCAGCTGCAGCCAAGAACAAAAC
AGGGAGTAAACCTCGAACCAGCGCTAACAGCAATAAAGATAAGGATAAAGATGAGAGAAAGTGGTTTAAA
GTACCTTCAAAGAAGGAGGAAACTTCAACTTGTATAGCCACACCAGACGTAGAGAAGAAGGAAGATCTGC
CTACATCTAGTGAAACATTTGGACTTCATGTAGAGAACGTTCCAAAGATGGTCTTTCCACAGCCAGAGAG
CACATTATCAAACAAGAGGAAAAATAATCAAGGCAACTCGTTTCAGGCAAAGAGAGCTCGACTTAACAAG
ATTACTGGTTTGTTGGCATCCAAAGCTGTTGGGGTTGATGGTGCTGAAAAAAAGGAAGACTACAATGAAA
CAGCTCCAATGCTGGAGCAGGCGATTTCACCTAAACCTCAAAGTCAGAAAAAAAATGAAGCTGACATTAG
CAGTTCTGCCAACACTCAGAAACCTGCACTGTTATCCTCAACTTTGTCTTCAGGGAAGGCTCGCAGCAAG
AAATGCAAACATGAATCTGGAGATTCTTCTGGGTGTATAAAACCCCCTAAATCACCACTTTCCCCAGAAT
TAATACAAGTCGAGGATTTGACGCTTGTATCTCAGCTTTCTTCTTCAGTGATAAATAAAACTAGTCCTCC
ACAGCCTGTGAATCCCCCTAGACCTTTCAAGCATAGTGAGCGGAGAAGAAGATCTCAGCGTTTAGCCACC
TTACCCATGCCTGATGATTCTGTAGAAAAGGTTTCTTCTCCCTCTCCAGCCACTGATGGGAAAGTATTCT
CCATCAGTTCTCAAAATCAGCAAGAATCTTCAGTACCAGAGGTGCCTGATGTTGCACATTTGCCACTTGA
GAAGCTGGGACCCTGTCTCCCTCTTGACTTAAGTCGTGGTTCAGAAGTTACAGCACCGGTAGCCTCAGAT
TCCTCTTACCGTAATGAATGTCCCAGGGCAGAAAAAGAGGATACACAGATGCTTCCAAATCCTTCTTCCA
AAGCAATAGCTGATGGAAGAGGAGCTCCAGCAGCAGCAGGAATATCGAAAACAGAAAAAAAAGTGAAATT
GGAAGACAAAAGCTCAACAGCATTTGGTATCAGGAGTTGGGATTTCTCAGCACTGCTAATGAAGATCCCC
TCTTATAGTCCAATAAGCTTATCAGGACTTCCAGAGTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213315 representing NM_032205
Red=Cloning site Green=Tags(s)

MSKKPPNRPGITFEIGARLEALDYLQKWYPSRIEKIDYEEGKMLVHFERWSHRYDEWIYWDSNRLRPLER
PALRKEGLKDEEDFFDFKAGEEVLARWTDCRYYPAKIEAINKEGTFTVQFYDGVIRCLKRMHIKAMPEDA
KGQVKSQHPLSWCCPIDPAGSCNQSMGSEDWIALVKAAAAAAAKNKTGSKPRTSANSNKDKDKDERKWFK
VPSKKEETSTCIATPDVEKKEDLPTSSETFGLHVENVPKMVFPQPESTLSNKRKNNQGNSFQAKRARLNK
ITGLLASKAVGVDGAEKKEDYNETAPMLEQAISPKPQSQKKNEADISSSANTQKPALLSSTLSSGKARSK
KCKHESGDSSGCIKPPKSPLSPELIQVEDLTLVSQLSSSVINKTSPPQPVNPPRPFKHSERRRRSQRLAT
LPMPDDSVEKVSSPSPATDGKVFSISSQNQQESSVPEVPDVAHLPLEKLGPCLPLDLSRGSEVTAPVASD
SSYRNECPRAEKEDTQMLPNPSSKAIADGRGAPAAAGISKTEKKVKLEDKSSTAFGIRSWDFSALLMKIP
SYSPISLSGLPES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032205
ORF Size 1719 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032205.3, NP_115581.3
RefSeq Size 2286 bp
RefSeq ORF 1721 bp
Locus ID 51105
Cytogenetics 8q24.22
Protein Families Druggable Genome, Transcription Factors
MW 63.3 kDa
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Documents
Resources
"NM_032205" in other vectors (4)
SKU Description Size Price
RC213315L3 Lenti-ORF clone of PHF20L1 (Myc-DDK-tagged)-Human PHD finger protein 20-like 1 (PHF20L1), transcript variant 2 10 ug
$833.00
RC213315L4 Lenti-ORF clone of PHF20L1 (mGFP-tagged)-Human PHD finger protein 20-like 1 (PHF20L1), transcript variant 2 10 ug
$833.00
RG213315 PHF20L1 (tGFP-tagged) - Human PHD finger protein 20-like 1 (PHF20L1), transcript variant 2 10 ug
$733.00
SC317983 PHF20L1 (untagged)-Human PHD finger protein 20-like 1 (PHF20L1), transcript variant 2 10 ug
$588.00

Plasmid Map for RC213315

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213315
Circular map for RC213315

file-alt Citations

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