Apolipoprotein L 5 (APOL5) (NM_030642) Human Tagged ORF Clone

  • TrueORF®
SKU
RC213205
APOL5 (Myc-DDK-tagged)-Human apolipoprotein L, 5 (APOL5)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Apolipoprotein L 5
Synonyms APOL-V; APOLV
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213205 representing NM_030642
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATGTGGCAAACAAGGAAATTTGCAAGTTCCCGGTTCCAAGGTGTTACCTGGCTTGGGAGAAGGTT
GTAAAGAAATGTGGCTTCGAAAGGTAATCTACGGAGGTGAGGTCTGGGGGAAGTCCCCAGAACCTGAGTT
CCCCTCACTCGTGAACCTGTGCCAGAGTTGGAAAATTAACAATTTGATGTCAACTGTCCACAGTGATGAG
GCTGGTATGCTGTCCTACTTTCTGTTTGAAGAGCTGATGCGATGTGACAAAGATTCCATGCCAGATGGAA
ATCTGTCAGAGGAGGAAAAATTGTTTCTCTCATATTTTCCTTTGCACAAGTTTGAGCTAGAACAGAACAT
CAAAGAACTTAACACCCTTGCGGACCAAGTTGACACCACTCACGAGTTGCTTACCAAGACCAGCCTGGTG
GCCAGCTCTTCCGGGGCTGTTTCTGGGGTCATGAACATCCTGGGTTTGGCCCTAGCACCTGTGACAGCAG
GAGGCAGTCTCATGCTCTCAGCAACTGGGACAGGGTTGGGGGCAGCAGCTGCCATCACCAACATAGTAAC
AAATGTCTTAGAAAATAGAAGCAATTCAGCAGCAAGAGACAAAGCCAGCCGACTGGGGCCTCTGACAACA
TCACATGAGGCTTTCGGAGGAATAAATTGGTCTGAAATCGAGGCTGCTGGCTTTTGTGTTAATAAGTGTG
TAAAAGCTATCCAGGGCATCAAGGATCTTCATGCCTACCAGATGGCCAAATCCAACTCTGGCTTCATGGC
TATGGTCAAGAATTTTGTGGCCAAGAGACACATCCCTTTCTGGACGGCTAGAGGGGTGCAGAGAGCCTTT
GAGGGCACAACTCTGGCCATGACCAATGGTGCCTGGGTGATGGGTGCTGCTGGGGCTGGCTTCTTACTTA
TGAAAGACATGAGCAGCTTCCTGCAGAGCTGGAAGCACCTGGAGGATGGGGCAAGGACGGAGACAGCAGA
GGAACTGAGAGCACTTGCTAAGAAGCTGGAGCAGGAGCTGGACCGGCTCACCCAGCACCACCGGCACCTG
CCGCAGAAGGCGAGCCAGACCTGTTCCAGCTCCCGGGGCAGGGCTGTTCGAGGATCCCGTGTGGTTAAAC
CAGAAGGGTCTCGCTCACCTCTCCCCTGGCCTGTTGTGGAGCACCAGCCTAGGCTGGGCCCTGGCGTGGC
ACTGAGGACACCAAAGAGGACAGTCTCTGCCCCAAGGATGCTTGGCCACCAGCCAGCCCCACCAGCACCA
GCAAGAAAGGGGAGACAGGCCCCGGGAAGACACCGACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213205 representing NM_030642
Red=Cloning site Green=Tags(s)

MPCGKQGNLQVPGSKVLPGLGEGCKEMWLRKVIYGGEVWGKSPEPEFPSLVNLCQSWKINNLMSTVHSDE
AGMLSYFLFEELMRCDKDSMPDGNLSEEEKLFLSYFPLHKFELEQNIKELNTLADQVDTTHELLTKTSLV
ASSSGAVSGVMNILGLALAPVTAGGSLMLSATGTGLGAAAAITNIVTNVLENRSNSAARDKASRLGPLTT
SHEAFGGINWSEIEAAGFCVNKCVKAIQGIKDLHAYQMAKSNSGFMAMVKNFVAKRHIPFWTARGVQRAF
EGTTLAMTNGAWVMGAAGAGFLLMKDMSSFLQSWKHLEDGARTETAEELRALAKKLEQELDRLTQHHRHL
PQKASQTCSSSRGRAVRGSRVVKPEGSRSPLPWPVVEHQPRLGPGVALRTPKRTVSAPRMLGHQPAPPAP
ARKGRQAPGRHRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030642
ORF Size 1299 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030642.1, NP_085145.1
RefSeq Size 1438 bp
RefSeq ORF 1302 bp
Locus ID 80831
UniProt ID Q9BWW9
Cytogenetics 22q12.3
MW 46.9 kDa
Summary This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_030642" in other vectors (4)
SKU Description Size Price
RC213205L3 Lenti ORF clone of Human apolipoprotein L, 5 (APOL5), Myc-DDK-tagged 10 ug
$757.00
RC213205L4 Lenti ORF clone of Human apolipoprotein L, 5 (APOL5), mGFP tagged 10 ug
$757.00
RG213205 APOL5 (tGFP-tagged) - Human apolipoprotein L, 5 (APOL5) 10 ug
$657.00
SC305277 APOL5 (untagged)-Human apolipoprotein L, 5 (APOL5) 10 ug
$503.00

Plasmid Map for RC213205

Download sequence for

Cloning scheme for RC213205

Circular map for RC213205

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