CLNS1A (NM_001293) Human Tagged ORF Clone

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SKU
RC213169
CLNS1A (Myc-DDK-tagged)-Human chloride channel, nucleotide-sensitive, 1A (CLNS1A)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CLNS1A
Synonyms CLCI; CLNS1B; ICln
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213169 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTTCCTCAAAAGTTTCCCGCCGCCTGGGCCAGCGGAGGGGCTCCTGCGGCAGCAGCCAGACACTG
AGGCTGTGCTGAACGGGAAGGGCCTCGGCACTGGTACCCTTTACATCGCTGAGAGCCGCCTGTCTTGGTT
AGATGGCTCTGGATTAGGATTCTCACTGGAATACCCCACCATTAGTTTACATGCATTATCCAGGGACCGA
AGTGACTGTCTAGGAGAGCATTTGTATGTTATGGTGAATGCCAAATTTGAAGAAGAATCAAAAGAACCTG
TTGCTGATGAAGAAGAGGAAGACAGTGATGATGATGTTGAACCTATTACTGAATTTAGATTTGTGCCTAG
TGATAAATCAGCGTTGGAGGCAATGTTCACTGCAATGTGCGAATGCCAGGCCTTGCATCCAGATCCTGAG
GATGAGGATTCAGATGACTACGATGGAGAAGAATATGATGTGGAAGCACATGAACAAGGACAGGGGGACA
TCCCTACATTTTACACCTATGAAGAAGGATTATCCCATCTAACAGCAGAAGGCCAAGCCACACTGGAGAG
ATTAGAAGGAATGCTTTCTCAGTCTGTGAGCAGCCAGTATAATATGGCTGGGGTCAGGACAGAAGATTCA
ATAAGAGATTATGAAGATGGGATGGAGGTGGATACCACACCAACAGTTGCTGGACAGTTTGAGGATGCAG
ATGTTGATCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213169 protein sequence
Red=Cloning site Green=Tags(s)

MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDR
SDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPE
DEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDS
IRDYEDGMEVDTTPTVAGQFEDADVDH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001293
ORF Size 711 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001293.3
RefSeq Size 1378 bp
RefSeq ORF 714 bp
Locus ID 1207
UniProt ID P54105
Cytogenetics 11q14.1
Domains ICln_channel
Protein Families Ion Channels: Other
MW 26.2 kDa
Summary This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2015
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Documents
Resources
"NM_001293" in other vectors (6)
SKU Description Size Price
RC213169L1 Lenti ORF clone of Human chloride channel, nucleotide-sensitive, 1A (CLNS1A), Myc-DDK-tagged 10 ug
$600.00
RC213169L2 Lenti ORF clone of Human chloride channel, nucleotide-sensitive, 1A (CLNS1A), mGFP tagged 10 ug
$600.00
RC213169L3 Lenti ORF clone of Human chloride channel, nucleotide-sensitive, 1A (CLNS1A), Myc-DDK-tagged 10 ug
$600.00
RC213169L4 Lenti ORF clone of Human chloride channel, nucleotide-sensitive, 1A (CLNS1A), mGFP tagged 10 ug
$600.00
RG213169 CLNS1A (tGFP-tagged) - Human chloride channel, nucleotide-sensitive, 1A (CLNS1A) 10 ug
$489.00 $500.00
SC119328 CLNS1A (untagged)-Human chloride channel, nucleotide-sensitive, 1A (CLNS1A) 10 ug
$300.00

Plasmid Map for RC213169

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Cloning scheme for RC213169

Circular map for RC213169

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