CABYR (NM_138643) Human Tagged ORF Clone

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SKU
RC213127
CABYR (Myc-DDK-tagged)-Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 5
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CABYR
Synonyms CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86; CT88; FSP-2; FSP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213127 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTTCTTCAAAGCCCAGACTTGTCGTACCCTATGGCCTCAAGACTCTGCTCGAGGGAATTAGCAGAG
CTGTTCTCAAAACCAACCCATCAAACATCAACCAGTTTGCAGCAGCTTATTTTCAAGAACTTACTATGTA
TAGAGGGAATACTACTATGGATATAAAAGATCTGGTTAAACAATTTCATCAGATTAAAGTAGAGAAATGG
TCAGAAGGAACGACACCACAGAAGAAATTAGAATGTTTAAAAGAACCAGGAAAAACATCTGTAGAATCTA
AAGTACCTACCCAGATGGAAAAATCTACAGACACAGACGAGGACAATGTAACCAGAACAGAATATAGTGA
CAAAACCACCCAGTTTCCATCAGTTTATGCTGTGCCAGGCACTGAGCAAACGGAAGCAGTTGGTGGTCTT
TCTTCCAAACCAGCCACCCCTAAGACTACTACCCCACCCTCATCACCACCTCCAACAGCTGTCTCACCAG
AGTTTGCCTACGTCCCAGCTGACCCAGCTCAGCTTGCTGCTCAGATGTTAGCAATGGCAACAAGTGAACG
AGGACAACCACCACCATGTTCTAACATGTGGACCCTTTATTGTCTAACTGATAAGAATCAACAAGGTCAC
CCATCACCGCCACCTGCACCTGGGCCTTTTCCCCAAGCAACCCTCTATTTACCTAATCCTAAGGATCCAC
AGTTTCAGCAGCATCCACCAAAAGTCACTTTTCCAACTTATGTGATGGGCGACACCAAGAAGACCAGTGC
CCCACCTTTTATCTTAGTAGGCTCAAATGTTCAGGAAGCACAGGGATGGAAACCTCTTCCCGGACATGCT
GTCGTTTCACAGTCAGATGTCTTGAGATATGTTGCAATGCAAGTGCCCATTGCTGTTCCTGCAGATGAGA
AATACCAGAAACATACCCTAAGTCCCCAGAATGCTAATCCTCCAAGTGGACAAGATGTCCCCAGGCCAAA
AAGCCCTGTTTTCCTTTCTGTTGCTTTCCCAGTAGAAGATGTAGCTAAAAAAAGTTCAGGATCTGGTGAC
AAATGTGCTCCCTTTGGAAGTTACGGTATTGCTGGGGAGGTAACCGTGACTACTGCTCACAAACGTCGCA
AAGCAGAAACTGAAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213127 protein sequence
Red=Cloning site Green=Tags(s)

MISSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMDIKDLVKQFHQIKVEKW
SEGTTPQKKLECLKEPGKTSVESKVPTQMEKSTDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGL
SSKPATPKTTTPPSSPPPTAVSPEFAYVPADPAQLAAQMLAMATSERGQPPPCSNMWTLYCLTDKNQQGH
PSPPPAPGPFPQATLYLPNPKDPQFQQHPPKVTFPTYVMGDTKKTSAPPFILVGSNVQEAQGWKPLPGHA
VVSQSDVLRYVAMQVPIAVPADEKYQKHTLSPQNANPPSGQDVPRPKSPVFLSVAFPVEDVAKKSSGSGD
KCAPFGSYGIAGEVTVTTAHKRRKAETEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138643
ORF Size 1137 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 1202 bp
RefSeq ORF 846 bp
Locus ID 26256
UniProt ID O75952, O75952-5
Cytogenetics 18q11.2
MW 41.1 kDa
Summary To reach fertilization competence, spermatozoa undergo a series of morphological and molecular maturational processes, termed capacitation, involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_138643" in other vectors (4)
SKU Description Size Price
RC213127L3 Lenti ORF clone of Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC213127L4 Lenti ORF clone of Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 5, mGFP tagged 10 ug
$757.00
RG213127 CABYR (tGFP-tagged) - Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 5 10 ug
$657.00
SC110367 CABYR (untagged)-Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 5 10 ug
$300.00

Plasmid Map for RC213127

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Cloning scheme for RC213127

Circular map for RC213127

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