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USP20 (NM_001008563) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC213090L3
Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,136.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol USP20
Synonyms hVDU2; LSFR3A; VDU2
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC213090).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001008563
ORF Size 2739 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001008563.1
RefSeq Size 4459 bp
RefSeq ORF 2745 bp
Locus ID 10868
UniProt ID Q9Y2K6
Cytogenetics 9q34.11
Protein Families Druggable Genome, Protease
MW 101.9 kDa
Summary This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. provided by RefSeq, Jan 2013
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"NM_001008563" in other vectors (5)
SKU Description Size Price
RC213090 USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 10 ug
$836.00
RC213090L4 Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, mGFP tagged 10 ug
$1,136.00
RG213090 USP20 (tGFP-tagged) - Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 10 ug
$789.00 $1,036.00
SC301324 USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 10 ug
$921.00
SC328026 USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20) transcript variant 2 10 ug
$921.00

Plasmid Map for RC213090L3

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Cloning scheme for RC213090L3
Circular map for RC213090L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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