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SALL4 (NM_020436) Human Tagged ORF Clone

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SKU
RC213089
SALL4 (Myc-DDK-tagged)-Human sal-like 4 (Drosophila) (SALL4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,375.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SALL4
Synonyms DRRS; HSAL4; IVIC; ZNF797
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213089 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGGCGCAAGCAGGCGAAACCCCAGCACATCAACTCGGAGGAGGACCAGGGCGAGCAGCAGCCGC
AGCAGCAGACCCCGGAGTTTGCAGATGCGGCCCCAGCGGCGCCCGCGGCGGGGGAGCTGGGTGCTCCAGT
GAACCACCCAGGGAATGACGAGGTGGCGAGTGAGGATGAAGCCACAGTAAAGCGGCTTCGTCGGGAGGAG
ACGCACGTCTGTGAGAAATGCTGTGCGGAGTTCTTCAGCATCTCTGAGTTCCTGGAACATAAGAAAAATT
GCACTAAAAATCCACCTGTCCTCATCATGAATGACAGCGAGGGGCCTGTGCCTTCAGAAGACTTCTCCGG
AGCTGTACTGAGCCACCAGCCCACCAGTCCCGGCAGTAAGGACTGTCACAGGGAGAATGGCGGCAGCTCA
GAGGACATGAAGGAGAAGCCGGATGCGGAGTCTGTGGTGTACCTAAAGACAGAGACAGCCCTGCCACCCA
CCCCCCAGGACATAAGCTATTTAGCCAAAGGCAAAGTGGCCAACACTAACGTGACCTTGCAGGCACTACG
GGGCACCAAGGTGGCGGTGAATCAGCGGAGCGCGGATGCACTCCCTGCCCCCGTGCCTGGTGCCAACAGC
ATCCCGTGGGTCCTCGAGCAGATCTTGTGTCTGCAGCAGCAGCAGCTACAGCAGATCCAGCTCACCGAGC
AGATCCGCATCCAGGTGAACATGTGGGCCTCCCACGCCCTCCACTCAAGCGGGGCAGGGGCCGACACTCT
GAAGACCTTGGGCAGCCACATGTCTCAGCAGGTTTCTGCAGCTGTGGCTTTGCTCAGCCAGAAAGCTGGA
AGCCAAGGTCTGTCTCTGGATGCCTTGAAACAAGCCAAGCTACCTCACGCCAACATCCCTTCTGCCACCA
GCTCCCTGTCCCCAGGGCTGGCACCCTTCACTCTGAAGCCGGATGGGACCCGGGTGCTCCCGAACGTCAT
GTCCCGCCTCCCGAGCGCTTTGCTTCCTCAGGCCCCGGGCTCGGTGCTCTTCCAGAGCCCTTTCTCCACT
GTGGCACTAGACACATCCAAGAAAGGGAAGGGGAAGCCACCGAACATCTCCGCGGTGGATGTCAAACCCA
AAGACGAGGCGGCCCTCTACAAGCACAAGTGTAAGTACTGTAGCAAGGTTTTTGGGACTGATAGCTCCTT
GCAGATCCACCTCCGCTCCCACACTGGAGAGAGACCCTTCGTGTGCTCTGTCTGTGGTCATCGCTTCACC
ACCAAGGGCAACCTCAAGGTGCACTTTCACCGACATCCCCAGGTGAAGGCAAACCCCCAGCTGTTTGCCG
AGTTCCAGGACAAAGTGGCGGCCGGCAATGGCATCCCCTATGCACTCTCTGTACCTGACCCCATAGATGA
ACCGAGTCTTTCTTTAGACAGCAAACCTGTCCTTGTAACCACCTCTGTAGGGCTACCTCAGAATCTTTCT
TCGGGGACTAATCCCAAGGACCTCACGGGTGGCTCCTTGCCCGGTGACCTGCAGCCTGGGCCTTCTCCAG
AAAGTGAGGGTGGACCCACACTCCCTGGGGTGGGACCAAACTATAATTCCCCAAGGGCTGGTGGCTTCCA
AGGGAGTGGGACCCCTGAGCCAGGGTCAGAGACCCTGAAATTGCAGCAGTTGGTGGAGAACATTGACAAG
GCCACCACTGATCCCAACGAATGTCTCATTTGCCACCGAGTCTTAAGCTGTCAGAGCTCCCTCAAGATGC
ATTATCGCACCCACACCGGGGAGAGACCGTTCCAGTGTAAGATCTGTGGCCGAGCCTTTTCTACCAAAGG
TAACCTGAAGACACACCTTGGGGTTCACCGAACCAACACATCCATTAAGACGCAGCATTCGTGCCCCATC
TGCCAGAAGAAGTTCACTAATGCCGTGATGCTGCAGCAACATATTCGGATGCACATGGGCGGTCAGATTC
CCAACACGCCCCTGCCAGAGAATCCCTGTGACTTTACGGGTTCTGAGCCAATGACCGTGGGTGAGAACGG
CAGCACCGGCGCTATCTGCCATGATGATGTCATCGAAAGCATCGATGTAGAGGAAGTCAGCTCCCAGGAG
GCTCCCAGCAGCTCCTCCAAGGTCCCCACGCCTCTTCCCAGCATCCACTCGGCATCACCCACGCTAGGGT
TTGCCATGATGGCTTCCTTAGATGCCCCAGGGAAAGTGGGTCCTGCCCCTTTTAACCTGCAGCGCCAGGG
CAGCAGAGAAAACGGTTCCGTGGAGAGCGATGGCTTGACCAACGACTCATCCTCGCTGATGGGAGACCAG
GAGTATCAGAGCCGAAGCCCAGATATCCTGGAAACCACATCCTTCCAGGCACTCTCCCCGGCCAATAGTC
AAGCCGAAAGCATCAAGTCAAAGTCTCCCGATGCTGGGAGCAAAGCAGAGAGCTCCGAGAACAGCCGCAC
TGAGATGGAAGGTCGGAGCAGTCTCCCTTCCACGTTTATCCGAGCCCCGCCGACCTATGTCAAGGTTGAA
GTTCCTGGCACATTTGTGGGACCCTCGACATTGTCCCCAGGGATGACCCCTTTGTTAGCAGCCCAGCCAC
GCCGACAGGCCAAGCAACATGGCTGCACACGGTGTGGGAAGAACTTCTCCTCTGCTAGCGCTCTTCAGAT
CCACGAGCGGACTCACACTGGAGAGAAGCCTTTTGTGTGCAACATTTGTGGGCGAGCTTTTACCACCAAA
GGCAACTTAAAGGTTCACTACATGACACACGGGGCGAACAATAACTCAGCCCGCCGTGGAAGGAAGTTGG
CCATCGAGAACACCATGGCTCTGTTAGGTACGGACGGAAAAAGAGTCTCAGAAATCTTTCCCAAGGAAAT
CCTGGCCCCTTCAGTGAATGTGGACCCTGTTGTGTGGAACCAGTACACCAGCATGCTCAATGGCGGTCTG
GCCGTGAAGACCAATGAGATCTCTGTGATCCAGAGTGGGGGGGTTCCTACCCTCCCGGTTTCCTTGGGGG
CCACCTCCGTTGTGAATAACGCCACTGTCTCCAAGATGGATGGCTCCCAGTCGGGTATCAGTGCAGATGT
GGAAAAACCAAGTGCTACTGACGGCGTTCCCAAACACCAGTTTCCTCACTTCCTGGAAGAAAACAAGATT
GCGGTCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213089 protein sequence
Red=Cloning site Green=Tags(s)

MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREE
THVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSS
EDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANS
IPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAG
SQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFST
VALDTSKKGKGKPPNISAVDVKPKDEAALYKHKCKYCSKVFGTDSSLQIHLRSHTGERPFVCSVCGHRFT
TKGNLKVHFHRHPQVKANPQLFAEFQDKVAAGNGIPYALSVPDPIDEPSLSLDSKPVLVTTSVGLPQNLS
SGTNPKDLTGGSLPGDLQPGPSPESEGGPTLPGVGPNYNSPRAGGFQGSGTPEPGSETLKLQQLVENIDK
ATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTSIKTQHSCPI
CQKKFTNAVMLQQHIRMHMGGQIPNTPLPENPCDFTGSEPMTVGENGSTGAICHDDVIESIDVEEVSSQE
APSSSSKVPTPLPSIHSASPTLGFAMMASLDAPGKVGPAPFNLQRQGSRENGSVESDGLTNDSSSLMGDQ
EYQSRSPDILETTSFQALSPANSQAESIKSKSPDAGSKAESSENSRTEMEGRSSLPSTFIRAPPTYVKVE
VPGTFVGPSTLSPGMTPLLAAQPRRQAKQHGCTRCGKNFSSASALQIHERTHTGEKPFVCNICGRAFTTK
GNLKVHYMTHGANNNSARRGRKLAIENTMALLGTDGKRVSEIFPKEILAPSVNVDPVVWNQYTSMLNGGL
AVKTNEISVIQSGGVPTLPVSLGATSVVNNATVSKMDGSQSGISADVEKPSATDGVPKHQFPHFLEENKI
AVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020436
ORF Size 3159 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020436.5
RefSeq Size 3487 bp
RefSeq ORF 3162 bp
Locus ID 57167
UniProt ID Q9UJQ4
Cytogenetics 20q13.2
Protein Families ES Cell Differentiation/IPS, Stem cell - Pluripotency
MW 112.2 kDa
Summary This gene encodes a zinc finger transcription factor thought to play a role in the development of abducens motor neurons. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
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"NM_020436" in other vectors (6)
SKU Description Size Price
RC213089L1 Lenti ORF clone of Human sal-like 4 (Drosophila) (SALL4), Myc-DDK-tagged 10 ug
$1,675.00
RC213089L2 Lenti ORF clone of Human sal-like 4 (Drosophila) (SALL4), mGFP tagged 10 ug
$1,675.00
RC213089L3 Lenti ORF clone of Human sal-like 4 (Drosophila) (SALL4), Myc-DDK-tagged 10 ug
$1,675.00
RC213089L4 Lenti ORF clone of Human sal-like 4 (Drosophila) (SALL4), mGFP tagged 10 ug
$1,675.00
RG213089 SALL4 (tGFP-tagged) - Human sal-like 4 (Drosophila) (SALL4) 10 ug
$789.00 $1,575.00
SC304767 SALL4 (untagged)-Human sal-like 4 (Drosophila) (SALL4) 10 ug
$1,376.00

Plasmid Map for RC213089

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213089
Circular map for RC213089

file-alt Citations

SALL4 promotes gastric cancer progression via hexokinase II mediated glycolysis
Shao, Zhang, Zhang et al
Cancer Cell Int (2020) 20, 188 DOI: 10.1186/s12935-020-01275-y
Cited by 49
AI Summary: In this study, OriGene products including SALL4 shRNA (OriGene, #TL313249) and HK2 shRNA (OriGene, #TL313015) were used to knock down SALL4 and HK-2 gene expression in gastric cancer cells, enabling the authors to investigate the roles of these genes in cancer cell metabolism and progression.
FigureFigureFigure
Whole-exome sequencing reveals SALL4 variants in premature ovarian insufficiency: an update on genotype-phenotype correlations
Wang, Li, Cai et al
Hum Genet (2019) 138 (1), 83-92 DOI: 10.1007/s00439-018-1962-4
Cited by 39
AI Summary: In this study, OriGene products—including the SALL4 Human cDNA ORF Clone and SALL4 antibody—were used for in vitro functional assays to assess the expression and regulatory activity of SALL4 variants identified in premature ovarian insufficiency patients.
Significance of SALL4 as a drug‑resistant factor in lung cancer
Yanagihara, Kobayashi, Kuribayashi et al
Int. J. Oncol. (2015) 46 (4), 1527-34 DOI: 10.3892/ijo.2015.2866
Cited by 32
AI Summary: OriGene's pCMV6-SALL4 expression vector was used in this study to investigate the role of SALL4 in drug resistance in lung cancer cells.
Snippet: SALL4 expression vector. The vector, inserted with the full‑length SALL4 gene (pCMV6‑SALL4), was purchased from Origene Technologies, Inc. (Rockville, MD, uSA), and large‑scale preparation was performed using the competent cells. ... Cite SaveSaving
SALL4, a novel marker for human gastric carcinogenesis and metastasis
Zhang, Xu, Xu et al
Oncogene (2013)
Cited by 126
AI Summary: OriGene products, specifically the SALL4 expression plasmid (RC213089) and the pCMV6-Entry vector plasmid (PS100001), were used to generate stable SALL4-overexpressing gastric cancer cell lines, enabling the study of SALL4's role in gastric cancer progression and metastasis.
Snippet: Generation of stable SALL4-overexpressing cell line. Stable SALL4 overexpression in HGC-27 cells was achieved using SALL4 expression plasmid (RC213089) and pCMV6-Entry vector plasmid (PS100001) purchased from OriGene (OriGene, Rockville, MD, USA) ... Cite SaveSaving
Showing 1-4 of 4 papers.
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