ATHL1 (PGGHG) (NM_025092) Human Tagged ORF Clone

SKU
RC213062
ATHL1 (Myc-DDK-tagged)-Human ATH1, acid trehalase-like 1 (yeast) (ATHL1)
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$521.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATHL1
Synonyms ATHL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213062 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGGACACCAGCACCCCCAGACCTGACCCTTGGGGAAGGTGAGGAGGCTAGGACGTGGGACTTCC
TGACAGCGGTGGGCGGCAGCCAGGCTGAGGCTCAGGCCTGCCTCACTGAGGCCCTGCAGCTGCAGGCCAG
GGGAGCTCTGTATACGGCTCACGCACAGGCCTGGGCCCAGCTCTGGGTAGAATGTGGCTTGGACGTGGTG
GGGCCCCTGCAGCTGCGCCAGGCCCTGCGTGGCTCCCTCTACTACCTGCTCAGTGCCCTGCCCCAGCCCA
AGGCCCCAGGATACATCTGCCATGGCCTCAGTCCTGGGGGCCTCTCCAATGGGAGCCGTGAGGAATGCTA
CTGGGGCCACGTCTTCTGGGACCAGGACCTCTGGATGTTCCCGAGTATCCTGATGTTCCACCCAGAAGCC
GCCAGGGCCATCCTGGAGTACCGCATCCGCACGCTGGACGGGGCCCTGGAGAACGCCCAGAACCTGGGCT
ACCAGGGAGCCAAGTTTGCCTGGGAGAGTGCAGACTCCGGCCTAGAGGTTTGCCCTGAGGACATTTACGG
AGTCCAGGAGGTCCACGTCAACGGGGCCGTGGTGTTGGCCTTCGAGCTGTACTACCATACCACCCAGGAC
CTGCAGCTATTTCGAGAGGCTGGTGGCTGGGACGTGGTCAGGGCTGTGGCCGAGTTTTGGTGCAGTCGTG
TTGAGTGGAGCCCCAGGGAGGAAAAGTACCACCTGAGGGGAGTCATGTCCCCCGACGAGTACCATTCAGG
GGTCAACAACTCTGTGTACACCAACGTCCTGGTCCAGAACAGCCTGCGCTTTGCTGCTGCCCTGGCCCAG
GACCTGGGTCTTCCCATCCCCAGCCAGTGGCTGGCGGTGGCTGACAAGATCAAGGTACCCTTTGACGTGG
AGCAGAACTTCCACCCGGAGTTCGATGGGTATGAGCCTGGAGAGGTGGTGAAGCAGGCAGACGTCGTGCT
CCTGGGATACCCAGTCCCCTTCTCCCTGAGTCCTGATGTTCGCAGGAAAAATCTGGAGATTTACGAGGCT
GTGACGTCCCCCCAGGGCCCCGCCATGACCTGGAGCATGTTTGCTGTGGGCTGGATGGAGCTGAAGGACG
CAGTGCGGGCCCGGGGCCTCCTGGACAGGAGCTTTGCCAACATGGCTGAACCCTTCAAGGTGTGGACGGA
GAATGCAGACGGGTCAGGCGCTGTGAACTTCCTGACAGGCATGGGGGGCTTCCTGCAGGCGGTGGTCTTC
GGGTGCACGGGGTTCAGGGTCACCCGAGCGGGTGTGACCTTTGACCCTGTGTGTCTGTCGGGGATCTCCA
GAGTGAGCGTCTCCGGCATCTTCTACCAGGGGAACAAGCTCAACTTCTCTTTTTCCGAGGACTCCGTGAC
CGTGGAGGTCACAGCTCGAGCAGGGCCCTGGGCTCCTCACCTGGAGGCTGAGCTGTGGCCATCCCAGTCC
CGGCTCTCCCTGTTGCCAGGACACAAGGTCTCCTTTCCCCGCTCGGCTGGCCGGATACAAATGTCACCCC
CGAAGCTGCCTGGAAGTTCCAGCTCCGAGTTCCCTGGGAGGACTTTTTCAGATGTTAGGGACCCGCTCCA
GAGCCCCCTCTGGGTCACCCTGGGTTCCTCCAGCCCCACCGAGTCACTCACTGTGGACCCTGCCTCTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213062 protein sequence
Red=Cloning site Green=Tags(s)

MLWTPAPPDLTLGEGEEARTWDFLTAVGGSQAEAQACLTEALQLQARGALYTAHAQAWAQLWVECGLDVV
GPLQLRQALRGSLYYLLSALPQPKAPGYICHGLSPGGLSNGSREECYWGHVFWDQDLWMFPSILMFHPEA
ARAILEYRIRTLDGALENAQNLGYQGAKFAWESADSGLEVCPEDIYGVQEVHVNGAVVLAFELYYHTTQD
LQLFREAGGWDVVRAVAEFWCSRVEWSPREEKYHLRGVMSPDEYHSGVNNSVYTNVLVQNSLRFAAALAQ
DLGLPIPSQWLAVADKIKVPFDVEQNFHPEFDGYEPGEVVKQADVVLLGYPVPFSLSPDVRRKNLEIYEA
VTSPQGPAMTWSMFAVGWMELKDAVRARGLLDRSFANMAEPFKVWTENADGSGAVNFLTGMGGFLQAVVF
GCTGFRVTRAGVTFDPVCLSGISRVSVSGIFYQGNKLNFSFSEDSVTVEVTARAGPWAPHLEAELWPSQS
RLSLLPGHKVSFPRSAGRIQMSPPKLPGSSSSEFPGRTFSDVRDPLQSPLWVTLGSSSPTESLTVDPASE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_025092
ORF Size 1680 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025092.3, NP_079368.2
RefSeq Size 3270 bp
RefSeq ORF 2214 bp
Locus ID 80162
UniProt ID Q32M88
Cytogenetics 11p15.5
Domains Glyco_hydro_65m
MW 61.6 kDa
Summary Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.[UniProtKB/Swiss-Prot Function]
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RC213062L3 Lenti ORF clone of Human ATH1, acid trehalase-like 1 (yeast) (ATHL1), Myc-DDK-tagged 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.