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LGI3 (NM_139278) Human Tagged ORF Clone

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SKU
RC213016
LGI3 (Myc-DDK-tagged)-Human leucine-rich repeat LGI family, member 3 (LGI3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $510.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LGI3
Synonyms LGIL4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213016 representing NM_139278
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGCTGCGGGCCAGGGGGGGCCCGGGGCCGGGGCTGCTGGCGCTCTCCGCGCTCGGCTTCTGCC
TCATGCTGCAAGTCAGCGCTAAGAGGCCCCCCAAGACGCCCCCCTGCCCGCCCAGCTGCTCTTGCACCAG
GGACACCGCCTTCTGCGTGGACTCAAAGGCGGTGCCCAGGAACCTGCCCTCGGAGGTCATCTCCCTGACC
CTGGTGAATGCCGCCTTCTCAGAGATCCAGGATGGAGCGTTCTCCCACCTCCCGCTGCTGCAGTTCTTGT
TACTCAACTCCAACAAGTTTACACTGATTGGAGACAACGCCTTCACAGGACTGTCGCACCTGCAGTATCT
CTTCATTGAGAACAATGACATCTGGGCACTATCCAAGTTCACCTTCCGAGGACTCAAGTCCTTGACTCAC
CTCTCGCTGGCCAACAATAACCTGCAGACACTGCCCAGAGACATCTTCCGGCCCCTGGACATCCTGAATG
ACTTGGACCTGCGGGGCAACTCACTCAACTGTGACTGCAAGGTGAAGTGGTTGGTGGAGTGGCTGGCACA
CACCAACACCACGGTGGCACCCATCTACTGCGCCAGCCCGCCCCGCTTCCAGGAGCACAAGGTGCAGGAC
CTGCCGCTGCGGGAGTTCGATTGCATCACCACAGATTTTGTGTTGTACCAGACCCTGGCCTTCCCAGCAG
TGTCGGCTGAGCCCTTCCTCTACTCCAGTGACCTCTATTTGGCTCTGGCCCAGCCAGGAGTCAGTGCCTG
CACCATCCTGAAGTGGGACTATGTTGAGCGGCAGCTTCGAGACTATGATAGAATCCCAGCCCCCTCTGCA
GTGCACTGCAAGCCGATGGTGGTGGACAGCCAGCTGTACGTGGTCGTGGCCCAGCTGTTTGGCGGCTCTT
ACATTTACCACTGGGATCCCAACACCACGCGCTTCACCAGGCTGCAAGACATTGACCCGCAGCGCGTGCG
CAAGCCTAACGACCTAGAAGCCTTCCGCATCGACGGTGACTGGTACTTTGCCGTGGCTGACAGCTCCAAG
GCAGGCGCCACCAGCCTCTACCGCTGGCACCAGAATGGCTTCTACTCCCACCAGGCACTGCACCCCTGGC
ACCGTGACACCGACCTGGAGTTTGTGGACGGCGAGGGCAAGCCACGGCTGATTGTGTCCAGCAGCTCCCA
GGCACCCGTCATCTATCAGTGGAGTCGCACCCAGAAGCAGTTTGTGGCCCAGGGTGAGGTGACCCAGGTG
CCTGATGCCCAAGCTGTGAAACACTTTCGTGCCGGCCGCGACAGCTACCTGTGCCTCAGCCGCTACATTG
GCGACTCCAAGATCCTGCGCTGGGAGGGTACCCGCTTCTCGGAGGTGCAGGCCCTGCCCTCCCGGGGCTC
GCTGGCCCTGCAGCCCTTCCTTGTGGGTGGCCGCCGCTACCTGGCACTGGGCAGTGATTTCTCCTTCACC
CAGATCTACCAGTGGGATGAGGGACGACAGAAGTTTGTACGGTTCCAGGAGCTGGCTGTGCAGGCTCCTC
GGGCCTTCTGCTACATGCCTGCTGGGGACGCCCAGCTACTCCTGGCCCCCAGCTTCAAGGGACAGACGCT
GGTGTATAGACACATTGTGGTGGATCTCAGTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213016 representing NM_139278
Red=Cloning site Green=Tags(s)

MAGLRARGGPGPGLLALSALGFCLMLQVSAKRPPKTPPCPPSCSCTRDTAFCVDSKAVPRNLPSEVISLT
LVNAAFSEIQDGAFSHLPLLQFLLLNSNKFTLIGDNAFTGLSHLQYLFIENNDIWALSKFTFRGLKSLTH
LSLANNNLQTLPRDIFRPLDILNDLDLRGNSLNCDCKVKWLVEWLAHTNTTVAPIYCASPPRFQEHKVQD
LPLREFDCITTDFVLYQTLAFPAVSAEPFLYSSDLYLALAQPGVSACTILKWDYVERQLRDYDRIPAPSA
VHCKPMVVDSQLYVVVAQLFGGSYIYHWDPNTTRFTRLQDIDPQRVRKPNDLEAFRIDGDWYFAVADSSK
AGATSLYRWHQNGFYSHQALHPWHRDTDLEFVDGEGKPRLIVSSSSQAPVIYQWSRTQKQFVAQGEVTQV
PDAQAVKHFRAGRDSYLCLSRYIGDSKILRWEGTRFSEVQALPSRGSLALQPFLVGGRRYLALGSDFSFT
QIYQWDEGRQKFVRFQELAVQAPRAFCYMPAGDAQLLLAPSFKGQTLVYRHIVVDLSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139278
ORF Size 1644 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139278.4
RefSeq Size 3285 bp
RefSeq ORF 1647 bp
Locus ID 203190
UniProt ID Q8N145
Cytogenetics 8p21.3
MW 61.5 kDa
Summary May participate in the regulation of neuronal exocytosis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139278" in other vectors (6)
SKU Description Size Price
RC213016L1 Lenti ORF clone of Human leucine-rich repeat LGI family, member 3 (LGI3), Myc-DDK-tagged 10 ug
$810.00
RC213016L2 Lenti ORF clone of Human leucine-rich repeat LGI family, member 3 (LGI3), mGFP tagged 10 ug
$810.00
RC213016L3 Lenti ORF clone of Human leucine-rich repeat LGI family, member 3 (LGI3), Myc-DDK-tagged 10 ug
$810.00
RC213016L4 Lenti ORF clone of Human leucine-rich repeat LGI family, member 3 (LGI3), mGFP tagged 10 ug
$810.00
RG213016 LGI3 (tGFP-tagged) - Human leucine-rich repeat LGI family, member 3 (LGI3) 10 ug
$710.00
SC306138 LGI3 (untagged)-Human leucine-rich repeat LGI family, member 3 (LGI3) 10 ug
$562.00

Plasmid Map for RC213016

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213016
Circular map for RC213016

file-alt Citations

LGI1 antibodies alter Kv1.1 and AMPA receptors changing synaptic excitability, plasticity and memory
Petit-Pedrol, Sell, Planagumà et al
Brain (2018) 141 (11), 3144-3159 DOI: 10.1093/brain/awy253
AI Summary: OriGene’s human LGI1 plasmid (human sequence sc-116925) was used to transfect HEK293 cells, enabling the study of antibody interactions with LGI1 in this research on synaptic excitability and memory.
Snippet: See Debanne and El Far (doi:10.1093/brain/awy271) for a scientific commentary on this article.Anti-LGI1 encephalitis is the most frequent cause of autoimmune li.;HEK293 cells seeded in P100 dishes at a density of 2 106 cells were grown to a confluence of 70% and transfected with a human LGI1 plasmid (Origene, human sequence sc-116925) using LipofectamineTM 2000 (Invitrogen) as reported (Lai et al., 2010)
Showing 1-1 of 1 papers.
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