FBXL21 (NM_012159) Human Tagged ORF Clone

  • TrueORF®
SKU
RC212994
FBXL21 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL21
Synonyms FBL3B; Fbl21; FBXL3B; FBXL3P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212994 representing NM_012159
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGGAACAGTTTATCTGTTGAGAATAAAATTGTCCAGTTGTCAGGAGCAGCGAAACAGCCAAAAG
TTGGGTTCTACTCTTCTCTCAACCAGACTCATACACACACGGTTCTTCTAGACTGGGGGAGTTTGCCTCA
CCATGTAGTATTACAAATTTTTCAGTATCTTCCTTTACTAGATCGGGCCTGTGCATCTTCTGTATGTAGG
AGGTGGAATGAAGTTTTTCATATTTCTGACCTTTGGAGAAAGTTTGAATTTGAACTGAACCAGTCAGCTA
CTTCATCTTTTAAGTCCACTCATCCTGATCTCATTCAGCAGATCATTAAAAAGCATTTTGCTCATCTTCA
GTATGTCAGCTTTAAGGTTGACAGTAGCGCTGAGTCAGCAGAAGCTGCCTGTGATATACTCTCTCAGCTG
GTAAATTGTTCCATCCAGACCTTGGGCTTGATTTCAACAGCCAAGCCAAGTTTCATGAATGTGTCGGAGT
CTCATTTTGTGTCAGCACTTACAGTTGTTTTTATCAACTCAAAATCATTATCATCAATCAAAATTGAAGA
TACACCAGTGGATGATCCTTCATTGAAGATTCTTGTGGCCAATAATAGTGACACTCTAAGACTCCCAAAG
ATGAGTAGCTGTCCTCATGTTTCATCTGATGGAATTCTTTGTGTAGCTGACCGTTGTCAAGGCCTTAGAG
AACTGGCGTTGAATTATTACATCCTAACTGATGAACTTTTCCTTGCACTCTCAAGCGAGACTCATGTTAA
CCTTGAACATCTTCGAATTGATGTTGTGAGTGAAAATCCTGGACAGATTAAATTTCATGCTGTTAAAAAA
CACAGTTGGGATGCACTTATTAAACATTCCCCTAGAGTTAATGTTGTTATGCACTTCTTTCTATATGAAG
AGGAATTCGAGACGTTCTTCAAAGAAGAAACCCCTGTTACTCACCTTTATTTTGGTCGTTCAGTCAGCAA
AGTGGTTTTAGGACGGGTAGGTCTCAACTGTCCTCGACTGATTGAGTTAGTGGTGTGTGCTAATGATCTT
CAGCCTCTTGATAATGAACTTATTTGTATTGCTGAACACTGTACAAACCTAACAGCCTTGGGCCTCAGCA
AATGTGAAGTTAGCTGCAGTGCCTTCATCAGGTTTGTAAGACTGTGTGAGAGAAGGTTAACACAGCTCTC
TGTAATGGAGGAAGTTTTGATCCCTGATGAGGATTATAGCCTAGATGAAATTCACACTGAAGTCTCCAAA
TACCTGGGAAGAGTATGGTTCCCTGATGTGATGCCTCTCTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC212994 representing NM_012159
Red=Cloning site Green=Tags(s)

MKRNSLSVENKIVQLSGAAKQPKVGFYSSLNQTHTHTVLLDWGSLPHHVVLQIFQYLPLLDRACASSVCR
RWNEVFHISDLWRKFEFELNQSATSSFKSTHPDLIQQIIKKHFAHLQYVSFKVDSSAESAEAACDILSQL
VNCSIQTLGLISTAKPSFMNVSESHFVSALTVVFINSKSLSSIKIEDTPVDDPSLKILVANNSDTLRLPK
MSSCPHVSSDGILCVADRCQGLRELALNYYILTDELFLALSSETHVNLEHLRIDVVSENPGQIKFHAVKK
HSWDALIKHSPRVNVVMHFFLYEEEFETFFKEETPVTHLYFGRSVSKVVLGRVGLNCPRLIELVVCANDL
QPLDNELICIAEHCTNLTALGLSKCEVSCSAFIRFVRLCERRLTQLSVMEEVLIPDEDYSLDEIHTEVSK
YLGRVWFPDVMPLW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012159
ORF Size 1302 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012159.4, NP_036291.2
RefSeq Size 1794 bp
RefSeq ORF 1305 bp
Locus ID 26223
Cytogenetics 5q31.1
MW 49.6 kDa
Summary This locus represents a transcribed pseudogene that is related to genes encoding members of the F-box family of proteins. provided by RefSeq, Nov 2017
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Documents
Resources
"NM_012159" in other vectors (7)
SKU Description Size Price
RC212994L1 Lenti-ORF clone of FBXL21 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$986.00
RC212994L2 Lenti-ORF clone of FBXL21 (mGFP-tagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$986.00
RC212994L3 Lenti-ORF clone of FBXL21 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$986.00
RC212994L4 Lenti-ORF clone of FBXL21 (mGFP-tagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$986.00
RG212994 FBXL21 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$886.00
SC100669 FBXL21 (untagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$450.00
SC317706 FBXL21 (untagged)-Human F-box and leucine-rich repeat protein 21 (gene/pseudogene) (FBXL21) 10 ug
$890.00

Plasmid Map for RC212994

Download sequence for

Cloning scheme for RC212994

Circular map for RC212994

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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