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PIAS2 (NM_004671) Human Tagged ORF Clone

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SKU
RC212659
PIAS2 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $578.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIAS2
Synonyms ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212659 representing NM_004671
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATTTCGAAGAGTTGAGGAATATGGTTTCTAGTTTTAGGGTTTCTGAACTACAAGTATTACTAG
GCTTTGCTGGACGGAATAAAAGTGGACGCAAGCATGACCTCCTGATGAGGGCGCTGCATTTATTGAAGAG
CGGCTGCAGCCCTGCGGTTCAGATTAAAATCCGAGAATTGTATAGACGCCGATATCCACGAACTCTTGAA
GGACTTTCTGATTTATCCACAATCAAATCATCGGTTTTCAGTTTGGATGGTGGCTCATCACCTGTAGAAC
CTGACTTGGCCGTGGCTGGAATCCACTCGTTGCCTTCCACTTCAGTTACACCTCACTCACCATCCTCTCC
TGTTGGTTCTGTGCTGCTTCAAGATACTAAGCCCACATTTGAGATGCAGCAGCCATCTCCCCCAATTCCT
CCTGTCCATCCTGATGTGCAGTTAAAAAATCTGCCCTTTTATGATGTCCTTGATGTTCTCATCAAGCCCA
CGAGTTTAGTTCAAAGCAGTATTCAGCGATTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAACA
AGTTAGAGAGATATGCATATCCAGGGATTTTTTGCCAGGTGGTAGGAGAGATTATACAGTCCAAGTTCAG
TTGAGACTTTGCCTGGCAGAGACAAGTTGCCCTCAAGAAGATAACTATCCAAATAGTCTATGTATAAAAG
TAAATGGGAAGCTATTTCCTTTGCCTGGCTATGCACCACCGCCTAAAAATGGGATTGAACAGAAGCGCCC
TGGACGCCCCTTGAATATTACATCTTTAGTTAGGTTATCTTCAGCTGTGCCAAACCAAATTTCCATTTCT
TGGGCATCAGAAATTGGGAAGAATTACTCTATGTCTGTATATCTTGTACGGCAGCTTACATCAGCCATGT
TATTACAGAGATTAAAAATGAAAGGTATTAGAAACCCTGATCATTCCAGAGCACTAATTAAAGAAAAACT
TACTGCAGATCCTGATAGTGAAATTGCTACAACTAGCCTTCGGGTATCCTTGATGTGCCCTTTAGGAAAA
ATGAGGCTGACAATCCCATGCCGTGCAGTGACTTGTACACATCTGCAGTGTTTTGATGCTGCCCTCTATC
TACAAATGAATGAGAAAAAGCCCACCTGGATTTGTCCTGTGTGTGACAAAAAAGCTGCCTATGAAAGTCT
AATATTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTAGATGAGATCAAATTCCAAGAA
GATGGTTCTTGGTGTCCAATGAGACCGAAGAAAGAAGCTATGAAAGTATCCAGCCAACCGTGTACAAAAA
TAGAAAGTTCAAGCGTCCTCAGTAAGCCTTGTTCAGTGACTGTAGCCAGTGAGGCAAGCAAGAAGAAAGT
AGATGTTATTGATCTTACAATAGAAAGCTCTTCTGACGAAGAGGAAGACCCTCCTGCCAAAAGGAAATGC
ATCTTTATGTCAGAAACACAAAGCAGCCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGG
TGCCCAGTGTGACTTCGGTTGATCCTGCTGCTATTCCGCCTTCATTAACAGACTACTCAGTACCATTCCA
CCATACGCCAATATCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGAT
CCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCA
CCACCACCAGCTCCCATGAAAGCAGTACTCATGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCAT
AACCAGCAGTGGAAGTAACATTCCTGACATCATCTCATTGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212659 representing NM_004671
Red=Cloning site Green=Tags(s)

MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLE
GLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIP
PVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQ
LRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISIS
WASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGK
MRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQE
DGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKC
IFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVD
PQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004671
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004671.5
RefSeq Size 2360 bp
RefSeq ORF 1866 bp
Locus ID 9063
UniProt ID O75928
Cytogenetics 18q21.1
Domains SAP, zf-MIZ
Protein Families Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
Protein Pathways Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
MW 68.1 kDa
Summary This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. provided by RefSeq, Aug 2017
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"NM_004671" in other vectors (6)
SKU Description Size Price
RC212659L1 Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, Myc-DDK-tagged 10 ug
$878.00
RC212659L2 Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, mGFP tagged 10 ug
$878.00
RC212659L3 Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, Myc-DDK-tagged 10 ug
$878.00
RC212659L4 Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, mGFP tagged 10 ug
$878.00
RG212659 PIAS2 (tGFP-tagged) - Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta 10 ug
$489.00 $778.00
SC125358 PIAS2 (untagged)-Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta 10 ug
$579.00

Plasmid Map for RC212659

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212659
Circular map for RC212659

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