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MSH5 (NM_172166) Human Tagged ORF Clone

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SKU
RC212608
MSH5 (Myc-DDK-tagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $764.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MSH5
Synonyms G7; MUTSH5; NG23; POF13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212608 representing NM_172166
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCTTAGGAGCGAACCCAAGGAGGACACCGCAGGGACCGAGACCTGGGGCGGCCTCCTCCGGCT
TCCCCAGCCCGGCCCCAGTGCCGGGCCCCAGGGAGGCCGAGGAGGAGGAAGTCGAGGAGGAGGAGGAGCT
GGCCGAGATCCATCTGTGTGTGCTGTGGAATTCAGGATACTTGGGCATTGCCTACTATGATACTAGTGAC
TCCACTATCCACTTCATGCCAGATGCCCCAGACCACGAGAGCCTCAAGCTTCTCCAGAGAGTTCTGGATG
AGATCAATCCCCAGTCTGTTGTTACGAGTGCCAAACAGGATGAGAATATGACTCGATTTCTGGGAAAGCT
TGCCTCCCAGGAGCACAGAGAGCCTAAAAGACCTGAAATCATATTTTTGCCAAGTGTGGATTTTGGTCTG
GAGATAAGCAAACAACGCCTCCTTTCTGGAAACTACTCCTTCATCCCAGACGCCATGACTGCCACTGAGA
AAATCCTCTTCCTCTCTTCCATTATTCCCTTTGACTGCCTCCTCACAGTTCGAGCACTTGGAGGGCTGCT
GAAGTTCCTGGGTCGAAGAAGAATCGGGGTTGAACTGGAAGACTATAATGTCAGCGTCCCCATCCTGGGC
TTTAAGAAATTTATGTTGACTCATCTGGTGAACATAGATCAAGACACTTACAGTGTTCTACAGATTTTTA
AGAGTGAGTCTCACCCCTCAGTGTACAAAGTGGCCAGTGGACTGAAGGAGGGGCTCAGCCTCTTTGGAAT
CCTCAACAGATGCCACTGTAAGTGGGGAGAGAAGCTGCTCAGGCTATGGTTCACACGTCCGACTCATGAC
CTGGGGGAGCTCAGTTCTCGTCTGGACGTCATTCAGTTTTTTCTGCTGCCCCAGAATCTGGACATGGCTC
AGATGCTGCATCGGCTCCTGGGTCACATCAAGAACGTGCCTCTGATTCTGAAACGCATGAAGTTGTCCCA
CACCAAGGTCAGCGACTGGCAGGTTCTCTACAAGACTGTGTACAGTGCCCTGGGCCTGAGGGATGCCTGC
CGCTCCCTGCCGCAGTCCATCCAGCTCTTTCGGGACATTGCCCAAGAGTTCTCTGATGACCTGCACCATA
TCGCCAGCCTCATTGGGAAAGTAGTGGACTTTGAGGGCAGCCTTGCTGAAAATCGCTTCACAGTCCTCCC
CAACATAGATCCTGAAATTGATGAGAAAAAGCGAAGACTGATGGGACTTCCCAGTTTCCTTACTGAGGTT
GCCCGCAAGGAGCTGGAGAATCTGGACTCCCGTATTCCTTCATGCAGTGTCATCTACATCCCTCTGATTG
GCTTCCTTCTTTCTATTCCCCGCCTGCCTTCCATGGTAGAGGCCAGTGACTTTGAGATTAATGGACTGGA
CTTCATGTTTCTCTCAGAGGAGAAGCTGCACTATCGTAGTGCCCGAACCAAGGAGCTGGATGCATTGCTG
GGGGACCTGCACTGCGAGATCCGGGACCAGGAGACGCTGCTGATGTACCAGCTACAGTGCCAGGTGCTGG
CACGAGCAGCTGTCTTAACCCGAGTATTGGACCTTGCCTCCCGCCTGGACGTCCTGCTGGCTCTTGCCAG
TGCTGCCCGGGACTATGGCTACTCAAGGCCGCGTTACTCCCCACAAGTCCTTGGGGTACGAATCCAGAAT
GGCAGACATCCTCTGATGGAACTCTGTGCCCGAACCTTTGTGCCCAACTCCACAGAATGTGGTGGGGACA
AAGGGAGGGTCAAAGTCATCACTGGACCCAACTCATCAGGGAAGAGCATATACCTCAAACAGGTAGGCTT
GATCACATTCATGGCCCTGGTAGGCAGCTTTGTGCCAGCAGAGGAGGCCGAAATTGGGGCAGTAGACGCC
ATCTTCACACGAATTCATAGCTGCGAATCCATCTCCCTTGGCCTCTCCACCTTCATGATCGACCTCAACC
AGGTGGCGAAAGCAGTGAACAATGCCACTGCACAGTCGCTGGTCCTTATTGATGAATTTGGAAAGGGAAC
CAACACGGTGGATGGGCTCGCGCTTCTGGCCGCTGTGCTCCGACACTGGCTGGCACGTGGACCCACATGC
CCCCACATCTTTGTGGCCACCAACTTTCTGAGCCTTGTTCAGCTACAGCTGCTGCCACAAGGGCCCCTGG
TGCAGTATTTGACCATGGAGACCTGTGAGGATGGCAACGATCTTGTCTTCTTCTATCAGGTTTGCGAAGG
TGTTGCGAAGGCCAGCCATGCCTCCCACACAGCTGCCCAGGCTGGGCTTCCTGACAAGCTTGTGGCTCGT
GGCAAGGAGGTCTCAGACTTGATCCGCAGTGGAAAACCCATCAAGCCTGTCAAGGATTTGCTAAAGAAGA
ACCAAATGGAAAATTGCCAGACATTAGTGGATAAGTTTATGAAACTGGATTTGGAAGATCCTAACCTGGA
CTTGAACGTTTTCATGAGCCAGGAAGTGCTGCCTGCTGCCACCAGCATCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212608 representing NM_172166
Red=Cloning site Green=Tags(s)

MASLGANPRRTPQGPRPGAASSGFPSPAPVPGPREAEEEEVEEEEELAEIHLCVLWNSGYLGIAYYDTSD
STIHFMPDAPDHESLKLLQRVLDEINPQSVVTSAKQDENMTRFLGKLASQEHREPKRPEIIFLPSVDFGL
EISKQRLLSGNYSFIPDAMTATEKILFLSSIIPFDCLLTVRALGGLLKFLGRRRIGVELEDYNVSVPILG
FKKFMLTHLVNIDQDTYSVLQIFKSESHPSVYKVASGLKEGLSLFGILNRCHCKWGEKLLRLWFTRPTHD
LGELSSRLDVIQFFLLPQNLDMAQMLHRLLGHIKNVPLILKRMKLSHTKVSDWQVLYKTVYSALGLRDAC
RSLPQSIQLFRDIAQEFSDDLHHIASLIGKVVDFEGSLAENRFTVLPNIDPEIDEKKRRLMGLPSFLTEV
ARKELENLDSRIPSCSVIYIPLIGFLLSIPRLPSMVEASDFEINGLDFMFLSEEKLHYRSARTKELDALL
GDLHCEIRDQETLLMYQLQCQVLARAAVLTRVLDLASRLDVLLALASAARDYGYSRPRYSPQVLGVRIQN
GRHPLMELCARTFVPNSTECGGDKGRVKVITGPNSSGKSIYLKQVGLITFMALVGSFVPAEEAEIGAVDA
IFTRIHSCESISLGLSTFMIDLNQVAKAVNNATAQSLVLIDEFGKGTNTVDGLALLAAVLRHWLARGPTC
PHIFVATNFLSLVQLQLLPQGPLVQYLTMETCEDGNDLVFFYQVCEGVAKASHASHTAAQAGLPDKLVAR
GKEVSDLIRSGKPIKPVKDLLKKNQMENCQTLVDKFMKLDLEDPNLDLNVFMSQEVLPAATSIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172166
ORF Size 2502 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172166.1, NP_751898.1
RefSeq Size 3968 bp
RefSeq ORF 2505 bp
Locus ID 4439
UniProt ID O43196, A0A024RCM1
Cytogenetics 6p21.33
Protein Families Druggable Genome
MW 92.7 kDa
Summary This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. provided by RefSeq, Feb 2011
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"NM_172166" in other vectors (4)
SKU Description Size Price
RC212608L3 Lenti ORF clone of Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4, Myc-DDK-tagged 10 ug
$1,064.00
RC212608L4 Lenti ORF clone of Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4, mGFP tagged 10 ug
$1,064.00
RG212608 MSH5 (tGFP-tagged) - Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4 10 ug
$964.00
SC309519 MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4 10 ug
$841.00

Plasmid Map for RC212608

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212608
Circular map for RC212608

file-alt Citations

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