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EHM2 (EPB41L4B) (NM_019114) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC212424
EPB41L4B (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $824.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EHM2
Synonyms CG1; EHM2; LULU2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212424 representing NM_019114
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGGTTCCTGCGCCGGACCTTTGGCCGCCGCTCCATGCAGCGCTACGCGCGGGGCGCGGCGGGGC
GCGGGGCCGCCGGGCTGGGGGACGAGCGCGATGGGGGGCCACGGGGGGGCCCGGCCGCCGCCGCCTCCTC
CTCGGCGCTGCCCGCCGCGCCCGGGGGCAGCGTGTTCCCGGCGGGCGGCGGGCCCCTGCTCACCGGCGGC
GCGGCCGTGCACATCTCCGCCGCCGGCGCCGCCAAGGCCACCCTCTACTGCCGCGTCTTCCTGCTCGACG
GGACCGAAGTGAGCGTGGACCTGCCGAAACATGCCAAAGGCCAGGATTTGTTTGATCAGATTGTGTACCA
CTTGGACCTTGTGGAAACAGATTACTTTGGCCTCCAGTTCCTCGACTCTGCCCAGGTTGCGCACTGGCTG
GATCATGCCAAACCCATAAAAAAGCAGATGAAAATTGGACCTGCTTATGCTTTACACTTTCGAGTTAAAT
ACTATTCTTCAGAACCAAACAACCTTCGTGAGGAGTTTACAAGGTACCTGTTTGTTTTACAACTCAGGCA
TGACATTCTTTCTGGAAAATTGAAATGCCCTTATGAAACAGCTGTGGAATTAGCTGCTCTCTGTCTACAA
GCGGAGCTTGGGGAGTGCGAGCTTCCAGAACACACACCAGAGCTTGTGTCTGAGTTTCGGTTCATTCCAA
ATCAGACAGAAGCAATGGAATTTGATATCTTCCAGAGATGGAAAGAGTGCAGGGGAAAGAGCCCTGCCCA
GGCGGAACTCTCCTATCTGAATAAAGCGAAGTGGCTGGAAATGTATGGGGTAGACATGCACGTTGTCAGG
GGAAGAGATGGCTGTGAATATTCTCTTGGACTGACCCCGACAGGCATATTAATCTTTGAAGGAGCTAACA
AAATAGGCTTATTCTTTTGGCCTAAAATTACCAAAATGGATTTTAAAAAGAGCAAATTGACACTCGTGGT
GGTCGAGGATGATGATCAGGGACGTGAGCAAGAGCACACGTTTGTGTTCCGGTTAGACAGTGCCAGGACC
TGCAAACACCTTTGGAAGTGTGCAGTTGAGCACCACGCATTCTTCCGACTGCGGACGCCAGGAAACAGCA
AATCCAATAGATCCGACTTTATCAGGCTGGGCTCTCGCTTCAGATTCAGTGGGCGGACAGAATATCAAGC
TACACATGGCTCCAGGTTACGAAGAACCAGCACCTTTGAGAGGAAGCCTAGTAAACGTTATCCATCCCGG
AGACATTCAACGTTCAAAGCAAGCAACCCAGTGATAGCAGCCCAGCTCTGCTCTAAAACAAATCCAGAAG
TCCATAATTACCAGCCTCAATATCATCCTAATATCCATCCCAGCCAGCCCCGGTGGCATCCTCACTCTCC
AAATGTCAGCTACCCGCTCCCTTCCCCAGTGCTTAGCAGCTCGGACCGGTTGCCTTTTGGCATTGAGGAG
AATGGGGGCACACCGTTCCTCACCGCAGCTTCAGGAAGGCATCACCACCAGCACCAGCATCAGCATCAGC
ACCAGCACCACTCAAACTACAGCCTCTCACTGACCCTGGAGAACAAAGAGGGGCCTCTGAGGTCCCCAAA
CTCCAGCAGCAAGTCCCTTACAAAACTGAGTCCAGGAACACCTGCCTTGTTCAGTGAAGCCGCTGCCCAT
CTAAAGAAGCTGGAACTGGAAACTGTGAAGGCTGCTGGACCCTGGCCTCCTCTGCACATCAACATAAACA
AGGCTGAAGAAAAGAAAGTCTCGGAGAAAACTCTTCAGACTCCACTTTTGCCTTCCCCTGTTGCGGATCA
TGTGAAGTGTAACATTCTGAAAGCCCAGTTGGAAAATGCTTCCCGAGTGAACATCCAGGGTGGAAAGGAG
GAATCACCGTTTGTAAATATCAATAAGAAATCCAGTCTTCAGGACGCTAGTGTAAGAAGTCCTATTCCTA
TTCGTGTGGAAACTGCCCAGCCAGCTGTGGAAAAGCCGGAAATCAAGCCTCCCCGAGTGAGGAAGTTAAC
AAGACAGTATAGTTTTGATGAAGACGACCTCCCTCCAGACCTGGCCGAGGCAGTGGGAGTGACCACATCT
ACAACCACAAACACCACAACGGCCGCCACACAAGTCTCCGTGCCGCTGCCGTCCCCCAAGGTCCAGAATG
TCAGCTCGCCTCACAAGTCAGAAGGCAAAGGCCTGCTGTCCCCTGGGGCCAAGAGCCCCTCTGACCGAGG
AGGTGCCTTTACCCTGGAGCCGGGTGATCTTCTGATGGATTTCACAGAAGCCACTCCTCTGGCAGAGCCC
GCCAGCAACCCCCACTGTGCCCACTCTCGCTGTTCTCCTCCACTCTCTCTCCCCATGAAGGAAGAGACCA
CTGGAGTTTGCATGTACCCTCCAATCAAAACGAGGCTGATAAAAACATTCCCGGTTGATACAATGAACCC
GTTTCCTGATACTTTCACCACAGGGCCACAGTTTACTGCAGACTTCAGAGACAGTAAATTACAGTGCTGT
CCTGGCCCGACTTCCCCGCTGATCCCAGCAGCGACCCTGAGGCCTTTGACAGAGACCGTCTCCACAGTGC
AGACCATTTACACCACCCGGAAACCTGTTTCTCTGGCAGCCAGTGCAGAGACACTCCGGCAGGAACTGGA
GAGAGAGAAGATGATGAAAAGACTGTTGATGACCGAACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212424 representing NM_019114
Red=Cloning site Green=Tags(s)

MLRFLRRTFGRRSMQRYARGAAGRGAAGLGDERDGGPRGGPAAAASSSALPAAPGGSVFPAGGGPLLTGG
AAVHISAAGAAKATLYCRVFLLDGTEVSVDLPKHAKGQDLFDQIVYHLDLVETDYFGLQFLDSAQVAHWL
DHAKPIKKQMKIGPAYALHFRVKYYSSEPNNLREEFTRYLFVLQLRHDILSGKLKCPYETAVELAALCLQ
AELGECELPEHTPELVSEFRFIPNQTEAMEFDIFQRWKECRGKSPAQAELSYLNKAKWLEMYGVDMHVVR
GRDGCEYSLGLTPTGILIFEGANKIGLFFWPKITKMDFKKSKLTLVVVEDDDQGREQEHTFVFRLDSART
CKHLWKCAVEHHAFFRLRTPGNSKSNRSDFIRLGSRFRFSGRTEYQATHGSRLRRTSTFERKPSKRYPSR
RHSTFKASNPVIAAQLCSKTNPEVHNYQPQYHPNIHPSQPRWHPHSPNVSYPLPSPVLSSSDRLPFGIEE
NGGTPFLTAASGRHHHQHQHQHQHQHHSNYSLSLTLENKEGPLRSPNSSSKSLTKLSPGTPALFSEAAAH
LKKLELETVKAAGPWPPLHININKAEEKKVSEKTLQTPLLPSPVADHVKCNILKAQLENASRVNIQGGKE
ESPFVNINKKSSLQDASVRSPIPIRVETAQPAVEKPEIKPPRVRKLTRQYSFDEDDLPPDLAEAVGVTTS
TTTNTTTAATQVSVPLPSPKVQNVSSPHKSEGKGLLSPGAKSPSDRGGAFTLEPGDLLMDFTEATPLAEP
ASNPHCAHSRCSPPLSLPMKEETTGVCMYPPIKTRLIKTFPVDTMNPFPDTFTTGPQFTADFRDSKLQCC
PGPTSPLIPAATLRPLTETVSTVQTIYTTRKPVSLAASAETLRQELEREKMMKRLLMTEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019114
ORF Size 2700 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019114.4, NP_061987.3
RefSeq Size 5814 bp
RefSeq ORF 2703 bp
Locus ID 54566
UniProt ID Q9H329
Cytogenetics 9q31.3
Domains B41
MW 99.7 kDa
Summary Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019114" in other vectors (6)
SKU Description Size Price
RC212424L1 Lenti-ORF clone of EPB41L4B (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$1,124.00
RC212424L2 Lenti-ORF clone of EPB41L4B (mGFP-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$1,124.00
RC212424L3 Lenti-ORF clone of EPB41L4B (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$1,124.00
RC212424L4 Lenti-ORF clone of EPB41L4B (mGFP-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$1,124.00
RG212424 EPB41L4B (tGFP-tagged) - Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$1,024.00
SC125251 EPB41L4B (untagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2 10 ug
$837.00

Plasmid Map for RC212424

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212424
Circular map for RC212424

file-alt Citations

Differential expression and functions of Ehm2 transcript variants in lung adenocarcinoma
Li, Ma, Si et al
Int. J. Oncol. (2019)
AI Summary: OriGene's anti-human Ehm2/1 antibody (AP338037) was used in this study to detect and analyze the expression of the Ehm2/1 protein variant in lung adenocarcinoma tissues and cell lines.
Snippet: Primary antibodies against human Ehm2/1 (1:200;AP338037;OriGene Technologies, Inc., Rockville, MD, USA) and human Ehm2/2 (1:50;ab135616;Abcam, Cambridge, UK) were used for probing (incubation for 20 min at room temperature)
Showing 1-1 of 1 papers.
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