IRS2 (NM_003749) Human Tagged ORF Clone
$1,716.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IRS2 |
| Synonyms | IRS-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003749 |
| ORF Size | 4014 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq Size | 7014 bp |
| RefSeq ORF | 4017 bp |
| Locus ID | 8660 |
| UniProt ID | Q9Y4H2 |
| Cytogenetics | 13q34 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus |
| MW | 137.3 kDa |
| Summary | This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003749" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212413L1 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), Myc-DDK-tagged | 10 ug |
$2,016.00
|
|
| RC212413L2 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), mGFP tagged | 10 ug |
$2,016.00
|
|
| RC212413L3 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), Myc-DDK-tagged | 10 ug |
$2,016.00
|
|
| RC212413L4 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), mGFP tagged | 10 ug |
$2,016.00
|
|
| RG212413 | IRS2 (tGFP-tagged) - Human insulin receptor substrate 2 (IRS2) | 10 ug |
$1,916.00
|
|
| SC303374 | IRS2 (untagged)-Human insulin receptor substrate 2 (IRS2) | 10 ug |
$1,718.00
|
|
Citations
Recurrent Amplification at 13q34 Targets at CUL4A, IRS2, and TFDP1 As an Independent Adverse Prognosticator in Intrahepatic Cholangiocarcinoma
Liu, You, Weng et al
PLoS ONE (2015) 10 (12), e0145388 DOI: 10.1371/journal.pone.0145388
Liu, You, Weng et al
PLoS ONE (2015) 10 (12), e0145388 DOI: 10.1371/journal.pone.0145388
Cited by 10
AI Summary: In this study, OriGene products—including IRS2 cDNA (RC212413), specific siRNAs targeting IRS2, CUL4A, and TFDP1, and the PCNA antibody—were used for gene overexpression, gene silencing, and proliferation assays to investigate the roles of these genes in intrahepatic cholangiocarcinoma progression.
Showing 1-1 of 1 papers.
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