NBPF15 (NM_173638) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC212377L2
Lenti ORF clone of Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2, mGFP tagged
  $1,220.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NBPF15
Synonyms AB14; AG3; NBPF16
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC212377).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173638
ORF Size 2010 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173638.2, NP_775909.1
RefSeq Size 4272 bp
RefSeq ORF 2013 bp
Locus ID 284565
UniProt ID Q8N660
Cytogenetics 1q21.1
MW 77.5 kDa
Summary This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. provided by RefSeq, May 2013
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"NM_173638" in other vectors (6)
SKU Description Size Price
RC212377 NBPF15 (Myc-DDK-tagged)-Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2 10 ug
$920.00
RC212377L1 Lenti ORF clone of Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2, Myc-DDK-tagged 10 ug
$1,220.00
RC212377L3 Lenti ORF clone of Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2, Myc-DDK-tagged 10 ug
$1,220.00
RC212377L4 Lenti ORF clone of Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2, mGFP tagged 10 ug
$1,220.00
RG212377 NBPF15 (tGFP-tagged) - Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2 10 ug
$1,120.00
SC107071 NBPF15 (untagged)-Human neuroblastoma breakpoint family, member 15 (NBPF15), transcript variant 2 10 ug
$921.00

Plasmid Map for RC212377L2

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Cloning scheme for RC212377L2

Circular map for RC212377L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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