CYP3A7 (NM_000765) Human Tagged ORF Clone

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SKU
RC212353
CYP3A7 (Myc-DDK-tagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7)
  $702.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CYP3A7
Synonyms CP37; CYPIIIA7; P-450(HFL33); P-450111A7; P450-HFLA; P450HLp2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212353 representing NM_000765
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTCATCCCAAACTTGGCCGTGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGATACTCCTCTATC
TATATGGAACCCGTACACATGGACTTTTTAAGAAGCTTGGAATTCCAGGGCCCACACCTCTGCCTTTTTT
GGGAAATGCTTTGTCCTTCCGTAAGGGCTATTGGACGTTTGACATGGAATGTTATAAAAAGTATAGAAAA
GTCTGGGGTATTTATGACTGTCAACAGCCTATGCTGGCTATCACAGATCCCGACATGATCAAAACAGTGC
TAGTGAAAGAATGTTATTCTGTCTTCACAAACCGGAGGCCTTTCGGGCCAGTGGGATTTATGAAAAATGC
CATCTCTATAGCTGAGGATGAAGAATGGAAGAGAATACGATCATTGCTGTCTCCAACATTCACCAGCGGA
AAACTCAAGGAGATGGTCCCTATCATTGCCCAGTATGGAGATGTGTTGGTGAGAAATCTGAGGCGGGAAG
CAGAGACAGGCAAGCCTGTCACCTTGAAACACGTCTTTGGGGCCTACAGCATGGATGTGATCACTAGCAC
ATCATTTGGAGTGAGCATCGACTCTCTCAACAATCCACAAGACCCCTTTGTGGAAAACACCAAGAAGCTT
TTAAGATTTAATCCATTAGATCCATTCGTTCTCTCAATAAAAGTCTTTCCATTCCTTACCCCAATTCTTG
AAGCATTAAATATCACTGTGTTTCCAAGAAAAGTTATAAGTTTTCTAACAAAATCTGTAAAACAGATAAA
AGAAGGTCGCCTCAAAGAGACACAAAAGCACCGAGTGGATTTCCTTCAGCTGATGATTGACTCTCAGAAT
TCAAAAGACTCTGAGACCCACAAAGCTCTGTCTGATCTGGAGCTCATGGCCCAATCAATTATCTTTATTT
TTGCTGGCTATGAAACCACGAGCAGTGTTCTCTCCTTCATTATATATGAACTGGCCACTCACCCTGATGT
CCAGCAGAAAGTGCAGAAGGAAATTGATACAGTTTTACCCAATAAGGCACCACCCACCTATGATACTGTG
CTACAGTTGGAGTATCTTGACATGGTGGTGAATGAAACACTCAGATTATTCCCAGTTGCTATGAGACTTG
AGAGGGTCTGCAAAAAAGATGTTGAAATCAATGGGATGTTTATTCCCAAAGGGGTGGTGGTGATGATTCC
AAGCTATGTTCTTCATCATGACCCAAAGTACTGGAGAGAGCCTGAGAAGTTCCTCCCTGAAAGGTTCAGT
AAAAAGAACAAGGACAACATAGATCCTTACATATACACACCCTTTGGAAGTGGACCCAGAAACTGCATTG
GCATGAGGTTTGCTCTCGTGAACATGAAACTTGCTCTAGTCAGAGTCCTTCAGAACTTCTCCTTCAAACC
TTGTAAAGAAACACAGATCCCCCTGAAATTACGCTTTGGAGGACTTCTTCTAACAGAAAAACCCATTGTT
CTAAAGGCTGAGTCAAGGGATGAGACCGTAAGTGGAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC212353 representing NM_000765
Red=Cloning site Green=Tags(s)

MDLIPNLAVETWLLLAVSLILLYLYGTRTHGLFKKLGIPGPTPLPFLGNALSFRKGYWTFDMECYKKYRK
VWGIYDCQQPMLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKNAISIAEDEEWKRIRSLLSPTFTSG
KLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKHVFGAYSMDVITSTSFGVSIDSLNNPQDPFVENTKKL
LRFNPLDPFVLSIKVFPFLTPILEALNITVFPRKVISFLTKSVKQIKEGRLKETQKHRVDFLQLMIDSQN
SKDSETHKALSDLELMAQSIIFIFAGYETTSSVLSFIIYELATHPDVQQKVQKEIDTVLPNKAPPTYDTV
LQLEYLDMVVNETLRLFPVAMRLERVCKKDVEINGMFIPKGVVVMIPSYVLHHDPKYWREPEKFLPERFS
KKNKDNIDPYIYTPFGSGPRNCIGMRFALVNMKLALVRVLQNFSFKPCKETQIPLKLRFGGLLLTEKPIV
LKAESRDETVSGA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000765
ORF Size 1509 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000765.3, NP_000756.2
RefSeq Size 2095 bp
RefSeq ORF 1512 bp
Locus ID 1551
UniProt ID P24462
Cytogenetics 7q22.1
Domains p450
Protein Families Druggable Genome, P450, Transmembrane
Protein Pathways Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
MW 57.5 kDa
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. provided by RefSeq, Jan 2015
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Documents
Resources
"NM_000765" in other vectors (4)
SKU Description Size Price
RC212353L3 Lenti-ORF clone of CYP3A7 (Myc-DDK-tagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7) 10 ug
$1,002.00
RC212353L4 Lenti-ORF clone of CYP3A7 (mGFP-tagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7) 10 ug
$1,002.00
RG212353 CYP3A7 (tGFP-tagged) - Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7) 10 ug
$902.00
SC110865 CYP3A7 (untagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7) 10 ug
$704.00

Plasmid Map for RC212353

Download sequence for

Cloning scheme for RC212353

Circular map for RC212353

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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