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MUSK (NM_005592) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC212253
MUSK (Myc-DDK-tagged)-Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1
ORF Plasmid:
DDK DDK-His tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,193.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MUSK
Synonyms CMS9; FADS; FADS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212253 representing NM_005592
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAGCTCGTCAACATTCCACTGGTACATATTCTTACTCTGGTTGCCTTCAGCGGAACTGAGAAAC
TTCCAAAAGCTCCTGTCATCACCACTCCTCTTGAAACAGTGGATGCCTTAGTTGAAGAAGTGGCTACTTT
CATGTGTGCAGTGGAATCCTACCCCCAGCCTGAGATTTCCTGGACTAGAAATAAAATTCTCATTAAACTC
TTTGACACCCGGTACAGCATCCGGGAGAATGGGCAGCTCCTCACCATCCTGAGTGTGGAAGACAGTGATG
ATGGCATTTACTGCTGCACGGCCAACAATGGTGTGGGAGGAGCTGTGGAGAGTTGTGGAGCCCTGCAAGT
GAAGATGAAACCTAAAATAACTCGTCCTCCCATAAATGTGAAAATAATAGAGGGATTAAAAGCAGTCCTA
CCATGTACTACAATGGGTAATCCCAAACCATCAGTGTCTTGGATAAAGGGAGACAGCCCTCTCAGGGAAA
ATTCCCGAATTGCAGTTCTTGAATCTGGGAGCTTGAGGATTCATAACGTACAAAAGGAAGATGCAGGACA
GTATCGATGTGTGGCAAAAAACAGCCTCGGGACAGCATATTCCAAAGTGGTGAAGCTGGAAGTTGAGGTT
TTTGCCAGGATCCTGCGGGCTCCTGAATCCCACAATGTCACCTTTGGCTCCTTTGTGACCCTGCACTGTA
CAGCAACAGGCATTCCTGTCCCCACCATCACCTGGATTGAAAACGGAAATGCTGTTTCTTCTGGGTCCAT
TCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTGCAGCTGTTTATCACCAAGCCAGGACTCTAC
ACATGCATAGCTACCAATAAGCATGGGGAGAAGTTCAGTACTGCCAAGGCTGCAGCCACCATCAGCATAG
CAGAATGGAGTAAACCACAGAAAGATAACAAAGGCTACTGCGCCCAGTACAGAGGGGAGGTGTGTAATGC
AGTCCTGGCAAAAGATGCTCTTGTTTTTCTCAACACCTCCTATGCGGACCCTGAGGAGGCCCAAGAGCTA
CTGGTCCACACGGCCTGGAATGAACTGAAAGTAGTGAGCCCAGTCTGCCGGCCAGCTGCTGAGGCTTTGT
TGTGTAACCACATCTTCCAGGAGTGCAGTCCTGGAGTAGTGCCTACTCCTATTCCCATTTGCAGAGAGTA
CTGCTTGGCAGTAAAGGAGCTCTTCTGCGCAAAAGAATGGCTGGTAATGGAAGAGAAGACCCACAGAGGA
CTCTACAGATCCGAGATGCATTTGCTGTCCGTGCCAGAATGCAGCAAGCTTCCCAGCATGCATTGGGACC
CCACGGCCTGTGCCAGACTGCCACATCTAGATTATAACAAAGAAAACCTAAAAACATTCCCACCAATGAC
GTCCTCAAAGCCAAGTGTGGACATTCCAAATCTGCCTTCCTCCTCCTCTTCTTCCTTCTCTGTCTCACCT
ACATACTCCATGACTGTAATAATCTCCATCATGTCCAGCTTTGCAATATTTGTGCTTCTTACCATAACTA
CTCTCTATTGCTGCCGAAGAAGAAAACAATGGAAAAATAAGAAAAGAGAATCAGCAGCAGTAACCCTCAC
CACACTGCCTTCTGAGCTCTTACTAGATAGACTTCATCCCAACCCCATGTACCAGAGGATGCCGCTCCTT
CTGAACCCCAAATTGCTCAGCCTGGAGTATCCAAGGAATAACATTGAATATGTGAGAGACATCGGAGAGG
GAGCGTTTGGAAGGGTGTTTCAAGCAAGGGCACCAGGCTTACTTCCCTATGAACCTTTCACTATGGTGGC
AGTAAAGATGCTCAAAGAAGAAGCCTCGGCAGATATGCAAGCGGACTTTCAGAGGGAGGCAGCCCTCATG
GCAGAATTTGACAACCCTAACATTGTGAAGCTATTAGGAGTGTGTGCTGTCGGGAAGCCAATGTGCCTGC
TCTTTGAATACATGGCCTATGGTGACCTCAATGAGTTCCTCCGCAGCATGTCCCCTCACACCGTGTGCAG
CCTCAGTCACAGTGACTTGTCTATGAGGGCTCAGGTCTCCAGCCCTGGGCCCCCACCCCTCTCCTGTGCT
GAGCAGCTTTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCTTACCTCTCAGAACGTAAGTTTGTTCACC
GAGATTTAGCCACCAGGAACTGCCTGGTGGGCGAGAACATGGTGGTGAAAATTGCCGACTTTGGCCTCTC
CAGGAACATCTACTCAGCAGACTACTACAAAGCTAATGAAAACGACGCTATCCCTATCCGTTGGATGCCA
CCAGAGTCCATTTTTTATAACCGCTACACTACAGAGTCTGATGTGTGGGCCTATGGCGTGGTCCTCTGGG
AGATCTTCTCCTATGGCCTGCAGCCCTACTATGGGATGGCCCATGAGGAGGTCATTTACTACGTGCGAGA
TGGCAACATCCTCTCCTGCCCTGAGAACTGCCCCGTGGAGCTGTACAATCTCATGCGTCTATGTTGGAGC
AAGCTGCCTGCAGACAGACCCAGTTTCACCAGTATTCACCGAATTCTGGAACGCATGTGTGAGAGGGCAG
AGGGAACTGTGAGTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212253 representing NM_005592
Red=Cloning site Green=Tags(s)

MRELVNIPLVHILTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL
FDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL
PCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEV
FARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLY
TCIATNKHGEKFSTAKAAATISIAEWSKPQKDNKGYCAQYRGEVCNAVLAKDALVFLNTSYADPEEAQEL
LVHTAWNELKVVSPVCRPAAEALLCNHIFQECSPGVVPTPIPICREYCLAVKELFCAKEWLVMEEKTHRG
LYRSEMHLLSVPECSKLPSMHWDPTACARLPHLDYNKENLKTFPPMTSSKPSVDIPNLPSSSSSSFSVSP
TYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLL
LNPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALM
AEFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSMRAQVSSPGPPPLSCA
EQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGENMVVKIADFGLSRNIYSADYYKANENDAIPIRWMP
PESIFYNRYTTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILSCPENCPVELYNLMRLCWS
KLPADRPSFTSIHRILERMCERAEGTVSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005592
ORF Size 2607 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005592.4
RefSeq Size 2666 bp
RefSeq ORF 2610 bp
Locus ID 4593
UniProt ID O15146
Cytogenetics 9q31.3
Protein Families Druggable Genome, Protein Kinase, Transmembrane
MW 96.9 kDa
Summary This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.provided by RefSeq, Oct 2009
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Documents
Resources
"NM_005592" in other vectors (7)
SKU Description Size Price
RC212253L1 Lenti ORF clone of Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1, Myc-DDK-tagged 10 ug
$1,493.00
RC212253L2 Lenti ORF clone of Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1, mGFP tagged 10 ug
$1,493.00
RC212253L3 Lenti ORF clone of Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1, Myc-DDK-tagged 10 ug
$1,493.00
RC212253L4 Lenti ORF clone of Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1, mGFP tagged 10 ug
$1,493.00
RC600068 MUSK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens muscle, skeletal, receptor tyrosine kinase, transcript variant 1, Signal peptide (1-23) plus EC domain (24-495) 10 ug
$289.00 $457.00
RG212253 MUSK (tGFP-tagged) - Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1 10 ug
$1,393.00
SC127894 MUSK (untagged)-Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 1 10 ug
$1,195.00

Plasmid Map for RC212253

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212253
Circular map for RC212253

file-alt Citations

Improved plasma membrane expression of the trafficking defective P344R mutant of muscle, skeletal, receptor tyrosine kinase (MuSK) causing congenital myasthenic syndrome
Milhem, Al-Gazali, Ali
Int. J. Biochem. Cell Biol. (2015) 60C, 119-129 DOI: 10.1016/j.biocel.2014.12.015
Cited by 6
AI Summary: OriGene’s Human MUSK (NM_005592) cDNA ORF Clone was used as the template for generating MuSK missense mutants, enabling the study of trafficking defects and functional consequences of the P344R mutation in this research.
Snippet: missense mutants (D38E, P344R, M605I, A727 V, V790 M and M835 V) were introduced by QuickChange site-directed mutagenesis with Turbo PfuDNA polymerase (Stratagene) and using wild-type MUSK (NM_005592) Human cDNA ORF Clone (Origene ... Related articles All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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