PGC1 alpha (PPARGC1A) (NM_013261) Human Tagged ORF Clone
- TrueORF®
SKU
RC212244
PPARGC1A (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)
$1,096.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PGC1 alpha |
| Synonyms | LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013261 |
| ORF Size | 2394 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013261.5 |
| RefSeq Size | 6317 bp |
| RefSeq ORF | 2397 bp |
| Locus ID | 10891 |
| UniProt ID | Q9UBK2 |
| Cytogenetics | 4p15.2 |
| Domains | RRM |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Adipocytokine signaling pathway, Huntington's disease, Insulin signaling pathway |
| MW | 90.8 kDa |
| Summary | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013261" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212244L1 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A), Myc-DDK-tagged | 10 ug |
$1,396.00
|
|
| RC212244L2 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A), mGFP tagged | 10 ug |
$1,396.00
|
|
| RC212244L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A), Myc-DDK-tagged | 10 ug |
$1,396.00
|
|
| RC212244L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A), mGFP tagged | 10 ug |
$1,396.00
|
|
| RG212244 | PPARGC1A (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A) | 10 ug |
$1,296.00
|
|
| SC128185 | PPARGC1A (untagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A) | 10 ug |
$1,097.00
|
|
Citations
Enhanced Therapeutic Potential of the Secretome Released from Adipose-Derived Stem Cells by PGC-1α-Driven Upregulation of Mitochondrial Proliferation
Lee, Kim, Lee et al
Int J Mol Sci (2019) 20 (22) DOI: 10.3390/ijms20225589
Lee, Kim, Lee et al
Int J Mol Sci (2019) 20 (22) DOI: 10.3390/ijms20225589
AI Summary: OriGene’s pcDNA-PGC-1α plasmid was used to transfect adipose-derived stem cells, enabling the study’s investigation of how PGC-1α overexpression enhances the therapeutic effects of the stem cell secretome in liver injury models.
Snippet: ASCs were grown in 100 mm cell dishes (Corning Glass Works, Corning, NY, USA). After reaching 70-80% confluence, the ASCs were transiently transfected with 1 μg pcDNA-PGC-1α. The PGC-1α plasmid was purchased from OriGene Technologies (Rockville Cached
Aqueous extract of Houttuynia cordata ameliorates aortic endothelial injury during hyperlipidemia via FoxO1 and p38 MAPK pathway
Liu, Cao, Lv et al
Journal of Functional Foods (2019) 62, 103510 DOI: 10.1016/j.jff.2019.103510
Liu, Cao, Lv et al
Journal of Functional Foods (2019) 62, 103510 DOI: 10.1016/j.jff.2019.103510
AI Summary: In this study, the PGC-1α protein purchased from OriGene was used to investigate the molecular mechanisms by which Houttuynia cordata extract protects against hyperlipidemia-induced endothelial injury.
Snippet: Antibody against FoxO1 was purchased from Cell Signaling Technology (Beverly, MA, USA). Peroxisome proliferator receptor-γ coactivator 1α (PGC-1α) was purchased from OriGene Technology (Rockville, MD, USA). Complexes;
PGC-1α dictates endothelial function through regulation of eNOS expression
Craige, Kröller-Schön, Li et al
Sci Rep (2016) 6, 38210 DOI: 10.1038/srep38210
Craige, Kröller-Schön, Li et al
Sci Rep (2016) 6, 38210 DOI: 10.1038/srep38210
AI Summary: OriGene's human PGC-1α cDNA was used to generate expression constructs for investigating the role of PGC-1α in regulating endothelial function and eNOS expression in this study.
Snippet: Human PGC-1α cDNA (Origene, Rockville, MD) was linearized and inserted into pBSmVELacZ (Obtained from Kenneth Walsh, Ph.D. Boston University, Boston, MA) to replace the LacZ open reading frame through the NotI site to produce human PGC-1α expression ... Cite SaveSaving
Potential Role of PINK 1 for Increased PGC-1α-Mediated Mitochondrial Fatty Acid Oxidation and Their Associations with Alzheimer disease and Diabetes
Choi, Ravipati, Nimmagadda et al
Mitochondrion (2014)
Choi, Ravipati, Nimmagadda et al
Mitochondrion (2014)
Cited by 76
AI Summary: OriGene’s pCMV6 Myc-PGC-1α plasmid was used to generate stable PGC-1α-overexpressing HEK 293 cell lines, enabling the study of PGC-1α’s role in mitochondrial fatty acid oxidation and its association with Alzheimer’s disease and diabetes.
Snippet: To generate stable PGC-1α-overexpressing cell lines, HEK 293 cells were transfected with the plasmid pCMV6 Myc-PGC-1α (OriGene, Rockville, MD, USA) or control vector using Lipofectamine 2000 (0.10% (v/v) final, Invitrogen, Grand Island, NY, USA ) and selected ... Cite SaveSaving
AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes
Wang, Di, Noguchi
Int. J. Biochem. Cell Biol. (2014)
Wang, Di, Noguchi
Int. J. Biochem. Cell Biol. (2014)
Cited by 29
AI Summary: In this study, the PGC-1α expression vector from OriGene was used to investigate the role of PGC-1α in mediating erythropoietin’s effects on metabolic activity and reactive oxygen species production in white adipocytes.
Snippet: without EPO treatment. PGC-1α expression vector was purchased from Origene and transfected into 3T3-L1 adipocytes using Lipofectamine 2000 (Invitrogen). 2.3. NAD + /NADH determination Assays. NAD + and NADH levels ... Cite SaveSaving
Showing 1-5 of 5 papers.
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