CHX10 (VSX2) (NM_182894) Human Tagged ORF Clone

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SKU
RC212033
VSX2 (Myc-DDK-tagged)-Human visual system homeobox 2 (VSX2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHX10
Synonyms CHX10; HOX10; MCOP2; MCOPCB3; RET1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212033 representing NM_182894
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGGGAAAGCAGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAGTGGCCAAGAGTACCTCGGGGG
GCGCCCCGGCCAGGTGCACTGGGTTCGGCATCCAGGAGATCCTGGGCTTGAACAAGGAGCCCCCGAGCTC
CCACCCGCGGGCAGCGCTCGACGGCCTGGCCCCCGGGCACTTGCTGGCGGCGCGCTCAGTGCTCAGCCCC
GCGGGGGTGGGCGGCATGGGGCTTCTGGGGCCCGGGGGGCTCCCTGGCTTCTACACGCAGCCCACCTTCC
TGGAAGTGCTGTCCGACCCGCAGAGCGTCCACTTGCAGCCATTGGGCAGAGCATCGGGGCCGCTGGACAC
CAGCCAGACGGCCAGCTCGGATTCTGAAGATGTTTCCTCCAGCGATCGAAAAATGTCCAAATCTGCTTTA
AACCAGACCAAGAAACGGAAGAAGCGGCGACACAGGACAATCTTTACCTCCTACCAGCTAGAGGAGCTGG
AGAAGGCATTCAACGAAGCCCACTACCCAGACGTCTATGCCCGGGAGATGCTGGCCATGAAAACGGAGCT
GCCGGAAGACAGGATACAGGTCTGGTTCCAGAACCGTCGAGCCAAGTGGAGGAAGCGGGAGAAGTGCTGG
GGCCGGAGCAGTGTCATGGCGGAGTATGGGCTCTACGGGGCCATGGTGCGGCACTCCATCCCCCTGCCCG
AGTCCATCCTCAAGTCAGCCAAGGATGGCATCATGGACTCCTGTGCCCCGTGGCTACTGGGGATGCACAA
AAAGTCGCTGGAGGCAGCAGCCGAGTCGGGGAGGAAGCCCGAGGGGGAACGCCAGGCCCTGCCCAAGCTC
GACAAGATGGAGCAGGACGAGCGGGGCCCCGACGCTCAGGCGGCCATCTCCCAGGAGGAACTGAGGGAGA
ACAGCATTGCGGTGCTCCGGGCCAAAGCTCAGGAGCACAGCACCAAAGTGCTGGGGACTGTGTCTGGGCC
GGACAGCCTGGCCCGGAGTACCGAGAAGCCAGAGGAGGAGGAGGCCATGGATGAAGACAGGCCGGCGGAG
AGGCTCAGTCCACCGCAGCTGGAGGACATGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC212033 representing NM_182894
Red=Cloning site Green=Tags(s)

MTGKAGEALSKPKSETVAKSTSGGAPARCTGFGIQEILGLNKEPPSSHPRAALDGLAPGHLLAARSVLSP
AGVGGMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSAL
NQTKKRKKRRHRTIFTSYQLEELEKAFNEAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCW
GRSSVMAEYGLYGAMVRHSIPLPESILKSAKDGIMDSCAPWLLGMHKKSLEAAAESGRKPEGERQALPKL
DKMEQDERGPDAQAAISQEELRENSIAVLRAKAQEHSTKVLGTVSGPDSLARSTEKPEEEEAMDEDRPAE
RLSPPQLEDMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182894
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182894.3
RefSeq Size 3005 bp
RefSeq ORF 1086 bp
Locus ID 338917
UniProt ID P58304
Cytogenetics 14q24.3
Protein Families Transcription Factors
MW 39.2 kDa
Summary This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_182894" in other vectors (6)
SKU Description Size Price
RC212033L1 Lenti ORF clone of Human visual system homeobox 2 (VSX2), Myc-DDK-tagged 10 ug
$757.00
RC212033L2 Lenti ORF clone of Human visual system homeobox 2 (VSX2), mGFP tagged 10 ug
$757.00
RC212033L3 Lenti ORF clone of Human visual system homeobox 2 (VSX2), Myc-DDK-tagged 10 ug
$757.00
RC212033L4 Lenti ORF clone of Human visual system homeobox 2 (VSX2), mGFP tagged 10 ug
$757.00
RG212033 VSX2 (tGFP-tagged) - Human visual system homeobox 2 (VSX2) 10 ug
$657.00
SC307527 VSX2 (untagged)-Human visual system homeobox 2 (VSX2) 10 ug
$457.00

Plasmid Map for RC212033

Download sequence for

Cloning scheme for RC212033

Circular map for RC212033

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