OTOGL (NM_173591) Human Tagged ORF Clone

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SKU
RC212026
OTOGL (Myc-DDK-tagged)-Human otogelin-like (OTOGL)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OTOGL
Synonyms C12orf64; DFNB84B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212026 representing NM_173591
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCAAGTTCGACAGGAAGAACCTTGTTGTTTTTCCCCTTTTTGTGTTTGTGAATCTTGCACCAAAC
CTGTTCCACTATGTCATGATGGGGAATTTCTCACAGTAGATCTTAATAGCACACACTTCTGTTGTCCTCA
GTATTACTGTGTATGTGAACCAAACCTTTGTCCTATGCCATTACTCAACTGTGCAGAAGATATGAATCTT
GTGAAAGAAAATGTATCTGGTCAATGTTGCCCAACATGGCACTGTGAATGTAACTGTGAAAACCTTGTTA
TGCCAACTTGTGAAGTGGGAGAATTTACTGCAATAGACCATAACTTCCAGAGTGATTGTGGATGCATACA
GTATCTCTGTGAAAAGGATGATGTGTGTGTATTTCAAGAAGTATCAGTATTGAATCCTGGACAATCCATG
ATAAAGTATTTGGAAGAAGACTTTTGTTATACTATAGAGTGTCTGGAAGAAAAAGATAACCATACAGGCT
TTCACACTCTGAATTTTACACTGGTGAATTGTTCAAAAAAATGTGATGTTCACCAGGTATATACTCCATC
CCCAAGTGATTATGGTTGTTGTGGTACCTGCAAAAATGTATCCTGCAAATTTCACATGGAAAATGGAACA
TCAGTTGTATACGCGGTAGGGAGTACCTGGCACTACAATTGCACCACATATGAATGTGTTAAAACTGATG
AAGGAGCAATAATTCTGAACTACACAATGGTCTGTCCCCCTTTTAATGAGACTGAATGCAAAATGAATGA
AGGGATTGTGAAGCTTTATAATGAAGGCTGTTGCAAGATCTGCAAACGAGAAGAAAGAATATGCCAGAAA
GTGATCATTGAATCGGTCATAAGGAAACAGGACTGTATGAGCCAAAGCCCTATAAATGTTGCATCTTGTG
ACGGCAAATGCCCATCAGCTACCATATATAACATCAATATTGAAAGTCACCTAAGATTCTGCAAGTGTTG
TCGTGAAAATGGAGTACGAAACTTGTCTGTGCCTCTGTATTGCTCAGGAAATGGCACTGAAATTATGTAC
ACTCTCCAGGAACCCATAGACTGTACATGCCAGTGGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC212026 representing NM_173591
Red=Cloning site Green=Tags(s)

MIQVRQEEPCCFSPFCVCESCTKPVPLCHDGEFLTVDLNSTHFCCPQYYCVCEPNLCPMPLLNCAEDMNL
VKENVSGQCCPTWHCECNCENLVMPTCEVGEFTAIDHNFQSDCGCIQYLCEKDDVCVFQEVSVLNPGQSM
IKYLEEDFCYTIECLEEKDNHTGFHTLNFTLVNCSKKCDVHQVYTPSPSDYGCCGTCKNVSCKFHMENGT
SVVYAVGSTWHYNCTTYECVKTDEGAIILNYTMVCPPFNETECKMNEGIVKLYNEGCCKICKREERICQK
VIIESVIRKQDCMSQSPINVASCDGKCPSATIYNINIESHLRFCKCCRENGVRNLSVPLYCSGNGTEIMY
TLQEPIDCTCQWN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173591
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173591.1, NP_775862.1
RefSeq Size 2361 bp
RefSeq ORF 7062 bp
Locus ID 283310
UniProt ID A0A0A0MSS2
Cytogenetics 12q21.31
MW 263.4 kDa
Summary The protein encoded by this gene belongs to the otogelin family. This gene is expressed in the inner ear of vertebrates with the highest level of expression seen at the embryonic stage and lowest in adult. Knockdown studies in zebrafish suggest that this gene is essential for normal inner ear function. Mutations in this gene are associated with autosomal recessive deafness. provided by RefSeq, Dec 2012
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Documents
Resources
"NM_173591" in other vectors (3)
SKU Description Size Price
RC212026L3 Lenti-ORF clone of OTOGL (Myc-DDK-tagged)-Human otogelin-like (OTOGL) 10 ug
$757.00
RC212026L4 Lenti-ORF clone of OTOGL (mGFP-tagged)-Human otogelin-like (OTOGL) 10 ug
$757.00
RG212026 OTOGL (tGFP-tagged) - Human otogelin-like (OTOGL) 10 ug
$657.00

Plasmid Map for RC212026

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Cloning scheme for RC212026

Circular map for RC212026

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