DCTN1 (NM_004082) Human Tagged ORF Clone

SKU RC211975

DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DCTN1
• Synonyms: DAP-150; DP-150; P135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211975 representing NM_004082; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCACAGAGCAAGAGGCACGTGTACAGCCGGACGCCCAGCGGCAGCAGGATGAGTGCGGAGGCAAGCG; CCCGGCCTCTGCGGGTGGGCTCCCGTGTAGAGGTGATTGGAAAAGGCCACCGAGGCACTGTGGCCTATGT; TGGAGCCACACTGTTTGCCACTGGCAAATGGGTAGGCGTGATTCTGGATGAAGCAAAGGGCAAAAATGAT; GGAACTGTTCAAGGCAGGAAGTACTTCACTTGTGATGAAGGGCATGGCATCTTTGTGCGCCAGTCCCAGA; TCCAGGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACACCTGATTCTTCTGCTTCAAAAGTCCT; CAAAAGAGAGGGAACTGATACAACTGCAAAGACTAGCAAACTGCGGGGACTGAAGCCTAAGAAGGCACCG; ACAGCCCGAAAGACCACAACTCGGCGACCCAAGCCCACGCGCCCAGCCAGTACTGGGGTGGCTGGGGCCA; GTAGCTCCCTGGGCCCCTCTGGCTCAGCGTCAGCAGGTGAGCTGAGCAGCAGTGAGCCCAGCACCCCGGC; TCAGACTCCGCTGGCAGCACCCATCATCCCCACGCCGGTCCTCACCTCTCCTGGAGCAGTCCCCCCGCTT; CCTTCCCCATCCAAGGAGGAGGAGGGACTAAGGGCTCAGGTGCGGGACCTGGAGGAGAAACTAGAGACCC; TGAGACTGAAACGGGCAGAAGACAAAGCAAAGCTAAAAGAGCTGGAGAAACACAAAATCCAGCTGGAGCA; GGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCCGACCTGCAGCGGCGCCTCAAGGAGGCGAGA; AAGGAAGCCAAGGAGGCGCTGGAGGCAAAGGAACGCTATATGGAGGAGATGGCTGATACTGCTGATGCCA; TTGAGATGGCCACTTTGGACAAGGAGATGGCTGAAGAGCGGGCTGAGTCCCTGCAGCAGGAGGTGGAGGC; ACTGAAGGAGCGGGTGGACGAGCTCACTACTGACTTAGAGATCCTCAAGGCTGAGATTGAAGAGAAGGGC; TCAGATGGCGCTGCATCCAGTTATCAGCTCAAGCAGCTTGAGGAGCAGAATGCCCGCCTGAAGGATGCCC; TGGTGAGGATGCGGGATCTTTCTTCCTCAGAGAAGCAGGAGCATGTGAAGCTCCAGAAGCTCATGGAAAA; GAAGAACCAAGAGCTGGAAGTTGTGAGGCAACAGCGGGAGCGTCTGCAGGAGGAGCTAAGCCAGGCAGAG; AGCACCATTGATGAGCTCAAGGAGCAGGTGGATGCTGCTCTGGGTGCTGAGGAGATGGTGGAGATGCTGA; CAGATCGGAACCTGAATCTGGAAGAGAAAGTGCGCGAGTTGAGGGAGACTGTGGGAGACTTGGAAGCGAT; GAATGAGATGAACGATGAGCTGCAGGAGAATGCACGTGAGACAGAACTGGAGCTGCGGGAGCAGCTGGAC; ATGGCAGGCGCGCGGGTTCGTGAGGCCCAGAAGCGTGTGGAGGCAGCCCAGGAGACGGTTGCAGACTACC; AGCAGACCATCAAGAAGTACCGCCAGCTGACCGCCCATCTACAGGATGTGAATCGGGAACTGACAAACCA; GCAGGAAGCATCTGTGGAGAGGCAACAGCAGCCACCTCCAGAGACCTTTGACTTCAAAATCAAGTTTGCT; GAGACTAAGGCCCATGCCAAGGCAATTGAGATGGAATTGAGGCAGATGGAGGTGGCCCAGGCCAATCGAC; ACATGTCCCTGCTGACAGCCTTCATGCCTGACAGCTTCCTTCGGCCAGGTGGGGACCATGACTGCGTTCT; GGTGCTGTTGCTCATGCCTCGTCTCATTTGCAAGGCAGAGCTGATCCGGAAGCAGGCCCAGGAGAAGTTT; GAACTAAGTGAGAACTGTTCAGAGCGGCCTGGGCTGCGAGGAGCTGCTGGGGAGCAACTCAGCTTTGCTG; CTGGACTGGTGTACTCGCTGAGCCTGCTGCAGGCCACGCTACACCGCTATGAGCATGCCCTCTCTCAGTG; CAGTGTGGATGTGTATAAGAAAGTGGGCAGCCTGTACCCTGAGATGAGTGCCCATGAGCGCTCCTTGGAT; TTCCTCATTGAACTGCTGCACAAGGATCAGCTGGATGAGACTGTCAATGTGGAGCCTCTCACCAAGGCCA; TCAAGTACTATCAGCATCTGTACAGCATCCACCTTGCCGAACAGCCTGAGGACTGTACTATGCAGCTGGC; TGACCACATTAAGTTCACGCAGAGTGCTCTGGACTGCATGAGTGTGGAGGTAGGACGGCTGCGTGCCTTC; TTGCAGGGTGGGCAGGAGGCTACAGATATTGCCCTCCTGCTCCGGGATCTGGAAACTTCATGCAGTGACA; TCCGCCAGTTCTGCAAGAAGATCCGAAGGCGAATGCCAGGGACAGATGCTCCTGGGATCCCAGCTGCACT; GGCCTTTGGACCACAGGTATCTGACACGCTCCTAGACTGCAGGAAACACTTGACGTGGGTCGTGGCTGTG; CTGCAGGAGGTGGCAGCTGCTGCTGCCCAGCTCATTGCCCCACTGGCAGAGAATGAGGGGCTACTTGTGG; CTGCTCTGGAGGAACTGGCTTTCAAAGCAAGCGAGCAGATCTATGGGACCCCCTCCAGCAGCCCCTATGA; GTGTCTGCGCCAGTCATGCAACATCCTCATCAGTACCATGAACAAGCTGGCCACAGCCATGCAGGAGGGG; GAGTATGATGCAGAGCGGCCCCCCAGCAAGCCTCCACCGGTTGAACTGCGGGCTGCTGCCCTTCGTGCAG; AGATCACAGATGCTGAAGGCCTGGGTTTGAAGCTCGAAGATCGAGAGACAGTTATTAAGGAGTTGAAGAA; GTCACTCAAGATTAAGGGAGAGGAGCTAAGTGAGGCCAATGTGCGGCTGAGCCTCCTGGAGAAGAAGTTG; GACAGTGCTGCCAAGGATGCAGATGAGCGCATCGAGAAAGTCCAGACTCGGCTGGAGGAGACCCAGGCAC; TGCTGCGAAAGAAGGAGAAAGAGTTTGAGGAGACAATGGATGCACTCCAGGCTGACATCGACCAGCTGGA; GGCAGAGAAGGCAGAACTAAAGCAGCGTCTGAACAGCCAGTCCAAACGCACGATTGAGGGACTCCGGGGC; CCTCCTCCTTCAGGCATTGCTACTCTGGTCTCTGGCATTGCTGGTGAAGAACAGCAGCGAGGAGCCATCC; CTGGGCAGGCTCCAGGGTCTGTGCCAGGCCCAGGGCTGGTGAAGGACTCACCACTGCTGCTTCAGCAGAT; CTCTGCCATGAGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCCTCAAGGGAGCCCAGATGAAG; GCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTATCCCATGAGGGCCCTGGCAGTGAGTTAC; CAGCTGGAGCGCTGTATCGTAAGACCAGCCAGCTGCTGGAGACATTGAATCAATTGAGCACACACACGCA; CGTAGTAGACATCACTCGCACCAGCCCTGCTGCCAAGAGCCCGTCGGCCCAACTTATGGAGCAAGTGGCT; CAGCTTAAGTCCCTGAGTGACACCGTCGAGAAGCTCAAGGATGAGGTCCTCAAGGAGACAGTATCTCAGC; GCCCTGGAGCCACAGTACCCACTGACTTTGCCACCTTCCCTTCATCAGCCTTCCTCAGGGCCAAGGAGGA; GCAGCAGGATGACACAGTCTACATGGGCAAAGTGACCTTCTCATGTGCGGCTGGTTTTGGACAGCGACAC; CGGCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCATCTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211975 representing NM_004082; Red=Cloning site Green=Tags(s); ; MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKND; GTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLRGLKPKKAP; TARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPL; PSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEAR; KEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKG; SDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAE; STIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLD; MAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFA; ETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKF; ELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLD; FLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAF; LQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAV; LQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEG; EYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKL; DSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRG; PPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMK; ASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVA; QLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRH; RLVLTQEQLHQLHSRLIS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004082
• ORF Size: 3834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004082.5
• RefSeq Size: 4992 bp
• RefSeq ORF: 3837 bp
• Locus ID: 1639
• UniProt ID: Q14203
• Cytogenetics: 2p13.1
• Domains: CAP_GLY, M
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 141.5 kDa
• Summary: This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]