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Polyserase3 (PRSS53) (NM_001039503) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC211724
PRSS53 (Myc-DDK-tagged)-Human protease, serine, 53 (PRSS53)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $515.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Polyserase3
Synonyms POL3S; UNQ308
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211724 representing NM_001039503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGGTGCTGGGGCCCAGTGCTGCTCATCGCGGGTGCCACAGTCCTCATGGAGGGTCTTCAAGCCG
CTCAGCGTGCCTGTGGACAGCGTGGCCCCGGCCCCCCCAAGCCTCAGGAGGGCAACACAGTCCCTGGCGA
GTGGCCCTGGCAGGCCAGTGTGAGGAGGCAAGGAGCCCACATCTGCAGCGGCTCCCTGGTGGCAGACACC
TGGGTCCTCACTGCTGCCCACTGCTTTGAAAAGGCAGCAGCAACAGAACTGAATTCCTGGTCAGTGGTCC
TGGGTTCTCTGCAGCGTGAGGGACTCAGCCCTGGGGCCGAAGAGGTGGGGGTGGCTGCCCTGCAGTTGCC
CAGGGCCTATAACCACTACAGCCAGGGCTCAGACCTGGCCCTGCTGCAGCTCGCCCACCCCACGACCCAC
ACACCCCTCTGCCTGCCCCAGCCCGCCCATCGCTTCCCCTTTGGAGCCTCCTGCTGGGCCACTGGCTGGG
ATCAGGACACCAGTGATGCTCCTGGGACCCTACGCAATCTGCGCCTGCGTCTCATCAGTCGCCCCACATG
TAACTGTATCTACAACCAGCTGCACCAGCGACACCTGTCCAACCCGGCCCGGCCTGGGATGCTATGTGGG
GGCCCCCAGCCTGGGGTGCAGGGCCCCTGTCAGGGAGATTCCGGGGGCCCTGTGCTGTGCCTCGAGCCTG
ACGGACACTGGGTTCAGGCTGGCATCATCAGCTTTGCATCAAGCTGTGCCCAGGAGGACGCTCCTGTGCT
GCTGACCAACACAGCTGCTCACAGTTCCTGGCTGCAGGCTCGAGTTCAGGGGGCAGCTTTCCTGGCCCAG
AGCCCAGAGACCCCGGAGATGAGTGATGAGGACAGCTGTGTAGCCTGTGGATCCTTGAGGACAGCAGGTC
CCCAGGCAGGAGCACCCTCCCCATGGCCCTGGGAGGCCAGGCTGATGCACCAGGGACAGCTGGCCTGTGG
CGGAGCCCTGGTGTCAGAGGAGGCGGTGCTAACTGCTGCCCACTGCTTCATTGGGCGCCAGGCCCCAGAG
GAATGGAGCGTAGGGCTGGGGACCAGACCGGAGGAGTGGGGCCTGAAGCAGCTCATCCTGCATGGAGCCT
ACACCCACCCTGAGGGGGGCTACGACATGGCCCTCCTGCTGCTGGCCCAGCCTGTGACACTGGGAGCCAG
CCTGCGGCCCCTCTGCCTGCCCTATCCTGACCACCACCTGCCTGATGGGGAGCGTGGCTGGGTTCTGGGA
CGGGCCCGCCCAGGAGCAGGCATCAGCTCCCTCCAGACAGTGCCCGTGACCCTCCTGGGGCCTAGGGCCT
GCAGCCGGCTGCATGCAGCTCCTGGGGGTGATGGCAGCCCTATTCTGCCGGGGATGGTGTGTACCAGTGC
TGTGGGTGAGCTGCCCAGCTGTGAGGGCCTGTCTGGGGCACCACTGGTGCATGAGGTGAGGGGCACATGG
TTCCTGGCCGGGCTGCACAGCTTCGGAGATGCTTGCCAAGGCCCCGCCAGGCCGGCGGTCTTCACCGCGC
TCCCTGCCTATGAGGACTGGGTCAGCAGTTTGGACTGGCAGGTCTACTTCGCCGAGGAACCAGAGCCCGA
GGCTGAGCCTGGAAGCTGCCTGGCCAACATAAGCCAACCAACCAGCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211724 representing NM_001039503
Red=Cloning site Green=Tags(s)

MKWCWGPVLLIAGATVLMEGLQAAQRACGQRGPGPPKPQEGNTVPGEWPWQASVRRQGAHICSGSLVADT
WVLTAAHCFEKAAATELNSWSVVLGSLQREGLSPGAEEVGVAALQLPRAYNHYSQGSDLALLQLAHPTTH
TPLCLPQPAHRFPFGASCWATGWDQDTSDAPGTLRNLRLRLISRPTCNCIYNQLHQRHLSNPARPGMLCG
GPQPGVQGPCQGDSGGPVLCLEPDGHWVQAGIISFASSCAQEDAPVLLTNTAAHSSWLQARVQGAAFLAQ
SPETPEMSDEDSCVACGSLRTAGPQAGAPSPWPWEARLMHQGQLACGGALVSEEAVLTAAHCFIGRQAPE
EWSVGLGTRPEEWGLKQLILHGAYTHPEGGYDMALLLLAQPVTLGASLRPLCLPYPDHHLPDGERGWVLG
RARPGAGISSLQTVPVTLLGPRACSRLHAAPGGDGSPILPGMVCTSAVGELPSCEGLSGAPLVHEVRGTW
FLAGLHSFGDACQGPARPAVFTALPAYEDWVSSLDWQVYFAEEPEPEAEPGSCLANISQPTSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039503
ORF Size 1659 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039503.2
RefSeq Size 1662 bp
RefSeq ORF 1662 bp
Locus ID 339105
UniProt ID Q2L4Q9
Cytogenetics 16p11.2
MW 58.2 kDa
Summary In vitro can degrade the fibrinogen alpha chain of as well as pro-urokinase-type plasminogen activator.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039503" in other vectors (4)
SKU Description Size Price
RC211724L3 Lenti-ORF clone of PRSS53 (Myc-DDK-tagged)-Human protease, serine, 53 (PRSS53) 10 ug
$815.00
RC211724L4 Lenti-ORF clone of PRSS53 (mGFP-tagged)-Human protease, serine, 53 (PRSS53) 10 ug
$815.00
RG211724 PRSS53 (tGFP-tagged) - Human protease, serine, 53 (PRSS53) 10 ug
$715.00
SC310882 PRSS53 (untagged)-Human protease, serine, 53 (PRSS53) 10 ug
$516.00

Plasmid Map for RC211724

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211724
Circular map for RC211724

file-alt Citations

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