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CELSR1 (NM_014246) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC211652
CELSR1 (Myc-DDK-tagged)-Human cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila) (CELSR1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK
  $2,443.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CELSR1
Synonyms ADGRC1; CDHF9; FMI2; HFMI2; LMPHM9; ME2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-HindIII Cloning Scheme for this gene | Plasmid Map
ACCN NM_014246
ORF Size 9042 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 11389 bp
RefSeq ORF 9045 bp
Locus ID 9620
UniProt ID Q9NYQ6
Cytogenetics 22q13.31
Protein Families Druggable Genome, Transmembrane
MW 329.5 kDa
Summary The protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. This particular member is a developmentally regulated, neural-specific gene which plays an unspecified role in early embryogenesis. provided by RefSeq, Jul 2008
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"NM_014246" in other vectors (3)
SKU Description Size Price
RC211652L1 Lenti ORF clone of Human cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila) (CELSR1), Myc-DDK-tagged 10 ug
$2,743.00
RG211652 CELSR1 (tGFP-tagged) - Human cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila) (CELSR1) 10 ug
$2,843.00
SC304071 CELSR1 (untagged)-Human cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila) (CELSR1) 10 ug
$2,687.00

Plasmid Map for RC211652

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Cloning scheme for RC211652
Circular map for RC211652

file-alt Citations

Genetic analysis of rare coding mutations in CELSR1-3 in Chinese Congenital Heart and Neural Tube Defects
Qiao, Liu, Li et al
Clin. Sci. (2016)
AI Summary: OriGene's Myc-tagged human CELSR1 plasmid (RC211652) was used as the template for site-directed mutagenesis to generate CELSR1 variants, enabling the functional analysis of identified mutations in this study.
Snippet: 2010). 207 Plasmids 208 Myc-tagged human CELSR1 was purchased fromOrigene Technologies (RC211652). We 209 then performed site-directed mutations CELSR1P870L with primers (F: 5'-GACAACGGC 210 ATCCTGCAGAAATCAGAC ... Cite SaveSaving
Genetic analysis of rare coding mutations of CELSR1-3 in congenital heart and neural tube defects in Chinese people
Qiao, Liu, Li et al
Clinical Science (2016) 130 (24), 2329-2340
AI Summary: OriGene's Myc-tagged human CELSR1 plasmid (RC211652) was used as the template for generating and analyzing site-directed CELSR1 mutations in the study.
Snippet: Myc-tagged human CELSR1 was purchased from Origene Technologies (RC211652). We then performed the site-directed mutation CELSR1 P870L with primers (forward: 5′-GACAACGGCATCCTGCAGAAATCAGAC-3′;reverse: 5′-GTCTGATTTCTGCAGGATGCCGTTGTC-3′)
Showing 1-2 of 2 papers.
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