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BCR (NM_004327) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC211635L4
Lenti-ORF clone of BCR (mGFP-tagged)-Human breakpoint cluster region (BCR), transcript variant 1
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,517.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BCR
Synonyms ALL; BCR1; CML; D22S11; D22S662; PHL
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC211635).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004327
ORF Size 3813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004327.3
RefSeq Size 4739 bp
RefSeq ORF 3816 bp
Locus ID 613
UniProt ID P11274
Cytogenetics 22q11.23
Domains C2, PH, RhoGAP, RhoGEF
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chronic myeloid leukemia, Pathways in cancer
MW 142.6 kDa
Summary A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Jan 2020
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"NM_004327" in other vectors (6)
SKU Description Size Price
RC211635 BCR (Myc-DDK-tagged)-Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,217.00
RC211635L1 Lenti-ORF clone of BCR (Myc-DDK-tagged)-Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,517.00
RC211635L2 Lenti-ORF clone of BCR (mGFP-tagged)-Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,517.00
RC211635L3 Lenti-ORF clone of BCR (Myc-DDK-tagged)-Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,517.00
RG211635 BCR (tGFP-tagged) - Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,417.00
SC125605 BCR (untagged)-Human breakpoint cluster region (BCR), transcript variant 1 10 ug
$1,107.00

Plasmid Map for RC211635L4

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Cloning scheme for RC211635L4
Circular map for RC211635L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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