HDAC4 (NM_006037) Human Tagged ORF Clone
$1,415.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HDAC4 |
| Synonyms | AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDACA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006037 |
| ORF Size | 3252 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006037.4 |
| RefSeq Size | 8459 bp |
| RefSeq ORF | 3255 bp |
| Locus ID | 9759 |
| UniProt ID | P56524 |
| Cytogenetics | 2q37.3 |
| Domains | Hist_deacetyl |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 118.9 kDa |
| Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006037" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211495L1 | Lenti ORF clone of Human histone deacetylase 4 (HDAC4), Myc-DDK-tagged | 10 ug |
$1,715.00
|
|
| RC211495L2 | Lenti ORF clone of Human histone deacetylase 4 (HDAC4), mGFP tagged | 10 ug |
$1,715.00
|
|
| RC211495L3 | Lenti ORF clone of Human histone deacetylase 4 (HDAC4), Myc-DDK-tagged | 10 ug |
$1,715.00
|
|
| RC211495L4 | Lenti ORF clone of Human histone deacetylase 4 (HDAC4), mGFP tagged | 10 ug |
$1,715.00
|
|
| RG211495 | HDAC4 (tGFP-tagged) - Human histone deacetylase 4 (HDAC4) | 10 ug |
$1,615.00
|
|
| SC125507 | HDAC4 (untagged)-Human histone deacetylase 4 (HDAC4) | 10 ug |
$1,416.00
|
|
Citations
Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1
Barbier-Torres, Beraza, Fernández-Tussy et al
Hepatology (2015)
Barbier-Torres, Beraza, Fernández-Tussy et al
Hepatology (2015)
Cited by 37
AI Summary: OriGene’s pCMV6-HDAC4 plasmid was used to overexpress HDAC4 in hepatocytes, enabling the study of HDAC4’s role in cholestatic liver injury in the absence of Prohibitin-1.
Snippet: WT and Phb1 KO hepatocytes were transfected with two μg of pCMV6-HDAC4 (Origene) using jetPRIME TM reagent (Polyplus). pcDNA3-LacZ (Invitrogen) was used as negative control
Proteomics Analysis of Human Obesity Reveals the Epigenetic Factor HDAC4 as a Potential Target for Obesity
Abu-Farha, Tiss, Abubaker et al
PLoS ONE (2013) 8 (9), e75342 DOI: 10.1371/journal.pone.0075342
Abu-Farha, Tiss, Abubaker et al
PLoS ONE (2013) 8 (9), e75342 DOI: 10.1371/journal.pone.0075342
Cited by 90
AI Summary: OriGene products contributed to this study by providing the pCMV-HDAC4 expression vector and the pCMV empty vector, which were used to investigate the functional role of HDAC4 in obesity-related cellular assays.
Snippet: the software annotation. Plasmid constructs. The expression vector encoding HDAC4 (pCMV-HDAC4) and the pCMV empty vector were purchased from OriGene (OriGene Technologies, Inc, Rockville, MD). Reporter plasmids
Showing 1-2 of 2 papers.
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