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TTC23 (NM_022905) Human Tagged ORF Clone

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SKU
RC211456
TTC23 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTC23
Synonyms HCC-8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211456 representing NM_022905
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAGAATCACAGGAAACCCACATATCCAACCACCTAGATGAAGTTGTTGCTGCTGTTAGCATCACTC
ATAGAAAGAAGTTCCAAAACAAGCTGCTTCAGACAGCACTATTCCAGCCTCCTCGAGAGAAACTCCACCT
CTGTGAAGAGAAAGCAAAGTCCTATTCCAACAGTCATGAGTACAAACAGGCCGTCCATGAGCTTGTGCGT
TGCGTAGCACTGACAAGAATTTGCTATGGAGACTCACATTGGAAACTAGCAGAGGCACATGTTAATCTGG
CTCAAGGCTACCTCCAGCTGAAAGGACTGTCACTGCAAGCAAAACAACATGCAGAAAAAGCCAGACAAAT
CCTCGCCAACTCCATTGTGCCTCCCTATAGTGAGAATACAGATGTTTTCAAGTTTTCCATTGAGCTTTTC
CATACCATGGGCAGAGCTTTACTCTCCCTTCAAAAATTTAAGGAAGCTGCAGAGAATTTGACAAAAGCAG
AGAGACTTTCAAAGGAGCTGCTACAATGTGGAAGAATTATAAAGGAAGAATGGATAGAAATTGAAGCACG
GATCAGATTATCATTTGCACAGGTGTATCAAGGTCAGAAGAAGTCAAAAGAAGCTTTGTCCCACTATCAA
GCAGCTTTGGAATATGTTGAGATCAGTAAAGGTGAAACAAGTCGTGAGTGTGTACCCATATTGAGAGAAT
TAGCAGGTGTAGAGCAAGCCCTGGGACTCCACGATGTATCCATCAACCACTTCCTCCAGGCACATCTCAT
CATCCTGAGTAGAAGCCCCTCTCAAGTGGAGGCAGCAGACTCGGCACACATCGTCGCCCATGCTGCTGTC
GCTTCAGGGAGACACGAGCACCATGATGTAGCTGAGCAGTATTTTCAAGAGAGCATGGCTCATCTTAAGG
ATTCTGAAGGGATGGGAAGAACCAAATTTCTTTCAATTCAAGATGAATTTTGCCATTTTCTACAAATGAC
TGGACAAAAAGAGAGAGCAACCTCGATCCTGAGAGAGTCCCTGGAAGCCAAAGTGGAAGCATTTGGCGAT
TTCAGTCCCGAGGTGGCAGAGACATACCGGCTCCTGGGAGGAGCAGACCTGGCGCAGGGGAACCACAGTG
GGGCCCGCAAGAAACTGAAGAAGTGTCTCCAGATCCAGACCCTCTTATATGGACCGCAGGACAAAAGGAC
TCTGGCCACCCAGCAGGCCATGGGCATGCTGTCCACGGCCCCCAAGGTTGCTTCGAAGCCAAGGCAGGCA
TCAAAAGCCAAAGTGGCCTTCTGCACCAGCATCCCTCAGGACACCCTGCTGGGGAAGGCCCGGCCCGGCA
CAACAGCAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211456 representing NM_022905
Red=Cloning site Green=Tags(s)

MQESQETHISNHLDEVVAAVSITHRKKFQNKLLQTALFQPPREKLHLCEEKAKSYSNSHEYKQAVHELVR
CVALTRICYGDSHWKLAEAHVNLAQGYLQLKGLSLQAKQHAEKARQILANSIVPPYSENTDVFKFSIELF
HTMGRALLSLQKFKEAAENLTKAERLSKELLQCGRIIKEEWIEIEARIRLSFAQVYQGQKKSKEALSHYQ
AALEYVEISKGETSRECVPILRELAGVEQALGLHDVSINHFLQAHLIILSRSPSQVEAADSAHIVAHAAV
ASGRHEHHDVAEQYFQESMAHLKDSEGMGRTKFLSIQDEFCHFLQMTGQKERATSILRESLEAKVEAFGD
FSPEVAETYRLLGGADLAQGNHSGARKKLKKCLQIQTLLYGPQDKRTLATQQAMGMLSTAPKVASKPRQA
SKAKVAFCTSIPQDTLLGKARPGTTAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022905
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022905.4, NP_075056.3
RefSeq Size 3562 bp
RefSeq ORF 1343 bp
Locus ID 64927
Cytogenetics 15q26.3
Domains TPR
MW 49.8 kDa
Summary Participates positively in the ciliary Hedgehog (Hh) signaling.UniProtKB/Swiss-Prot Function
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"NM_022905" in other vectors (5)
SKU Description Size Price
RC211456L3 Lenti ORF clone of Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC211456L4 Lenti ORF clone of Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2, mGFP tagged 10 ug
$757.00
RG211456 TTC23 (tGFP-tagged) - Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2 10 ug
$489.00 $657.00
SC112399 TTC23 (untagged)-Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2 10 ug
$457.00
SC317732 TTC23 (untagged)-Human tetratricopeptide repeat domain 23 (TTC23), transcript variant 2 10 ug
$503.00

Plasmid Map for RC211456

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211456
Circular map for RC211456

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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