AZI2 (NM_022461) Human Tagged ORF Clone

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SKU
RC211435
AZI2 (Myc-DDK-tagged)-Human 5-azacytidine induced 2 (AZI2), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AZI2
Synonyms AZ2; NAP1; TILP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211435 representing NM_022461
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCACTGGTAGAAGATGATATCTGTATTCTGAATCATGAAAAAGCCCATAAGAGAGATACAGTGA
CTCCAGTTTCAATATATTCAGGAGATGAATCTGTTGCTTCCCATTTTGCTCTTGTCACTGCATATGAAGA
CATCAAAAAACGACTTAAGGATTCAGAGAAAGAGAACTCTTTGTTAAAGAAGAGAATAAGATTTTTGGAA
GAAAAGCTAATAGCTCGATTTGAAGAAGAAACAAGTTCCGTGGGACGAGAACAAGTAAATAAGGCCTATC
ATGCATATCGAGAGGTTTGCATTGATAGAGATAATTTGAAGAGCAAACTGGACAAAATGAATAAAGACAA
CTCTGAATCTTTGAAAGTATTGAATGAGCAGCTACAATCTAAAGAAGTAGAACTCCTCCAGCTGAGGACA
GAGGTGGAAACTCAGCAGGTGATGAGGAATTTAAATCCACCTTCATCAAACTGGGAGGTGGAAAAGTTGA
GCTGTGACCTGAAGATCCATGGTTTGGAACAAGAGCTGGAACTGATGAGGAAAGAATGTAGCGATCTCAA
AATAGAACTACAGAAAGCCAAACAAACGGATCCATATCAGGAAGACAATCTGAAGAGCAGAGATCTCCAA
AAACTAAGCATTTCAAGTGATAATATGCAGCATGCATACTGGGAACTGAAGAGAGAAATGTCTAATTTAC
ATCTGGTGACTCAAGTACAAGCTGAACTACTAAGAAAACTGAAAACCTCAACTGCAATCAAGAAAGCCTG
TGCCCCTGTAGGATGCAGTGAAGACCTTGGAAGAGACAGCACAAAACTGCACTTGATGAATTTTACTGCA
ACATACACAAGACATCCCCCTCTCTTACCAAATGGCAAAGCTCTTTGTCATACCACATCTTCCCCTTTAC
CAGGAGATGTAAAGGTTTTATCAGAGAAAGCAATCCTCCAATCATGGACAGACAATGAGAGATCCATTCC
TAATGATGGTACATGCTTTCAGGAACACAGTTCTTATGGCAGAAATTCTCTGGAAGACAATTCCTGGGTA
TTTCCAAGTCCTCCTAAATCAAGTGAGACAGCATTTGGGGAAACTAAAACTAAAACTTTGCCTTTACCCA
ACCTTCCACCACTGCATTACTTGGATCAACATAATCAGAACTGCCTTTATAAGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211435 representing NM_022461
Red=Cloning site Green=Tags(s)

MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLE
EKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRT
EVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQ
KLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKACAPVGCSEDLGRDSTKLHLMNFTA
TYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGRNSLEDNSWV
FPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022461
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022461.5
RefSeq Size 3113 bp
RefSeq ORF 1179 bp
Locus ID 64343
UniProt ID Q9H6S1, C9JB40
Cytogenetics 3p24.1
Protein Pathways RIG-I-like receptor signaling pathway
MW 44.8 kDa
Summary AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 PubMed 14560022).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_022461" in other vectors (6)
SKU Description Size Price
RC211435L1 Lenti ORF clone of Human 5-azacytidine induced 2 (AZI2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211435L2 Lenti ORF clone of Human 5-azacytidine induced 2 (AZI2), transcript variant 1, mGFP tagged 10 ug
$757.00
RC211435L3 Lenti ORF clone of Human 5-azacytidine induced 2 (AZI2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211435L4 Lenti ORF clone of Human 5-azacytidine induced 2 (AZI2), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211435 AZI2 (tGFP-tagged) - Human 5-azacytidine induced 2 (AZI2), transcript variant 1 10 ug
$489.00 $657.00
SC112480 AZI2 (untagged)-Human 5-azacytidine induced 2 (AZI2), transcript variant 1 10 ug
$457.00

Plasmid Map for RC211435

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Cloning scheme for RC211435

Circular map for RC211435

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