KCNK9 (NM_016601) Human Tagged ORF Clone

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SKU
RC211314
KCNK9 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 9 (KCNK9)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCNK9
Synonyms K2p9.1; KT3.2; TASK-3; TASK3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211314 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGGCAGAACGTGCGGACTCTGTCCCTCATCGTCTGCACCTTCACCTACCTGCTGGTGGGCGCCG
CCGTGTTCGACGCCCTCGAGTCGGACCACGAGATGCGCGAGGAGGAGAAACTCAAAGCCGAGGAGATCCG
GATCAAGGGGAAGTACAACATCAGCAGCGAGGACTACCGGCAGCTGGAGCTGGTGATCCTGCAGTCGGAA
CCGCACCGCGCCGGCGTCCAGTGGAAATTCGCCGGCTCCTTCTACTTTGCGATCACGGTCATCACCACCA
TAGGTTATGGGCACGCTGCACCTGGCACCGATGCGGGCAAGGCCTTCTGCATGTTCTACGCCGTGCTGGG
CATCCCGCTGACACTGGTCATGTTCCAGAGCCTGGGCGAGCGCATGAACACCTTCGTGCGCTACCTGCTG
AAGCGCATTAAGAAGTGCTGTGGCATGCGCAACACTGACGTGTCTATGGAGAACATGGTGACTGTGGGCT
TCTTCTCCTGCATGGGGACGCTGTGCATCGGGGCGGCCGCCTTCTCCCAGTGTGAGGAGTGGAGCTTCTT
CCACGCCTACTACTACTGCTTCATCACGTTGACTACCATTGGGTTCGGGGACTACGTGGCCCTGCAGACC
AAGGGCGCCCTGCAGAAGAAGCCGCTCTACGTGGCCTTTAGCTTTATGTATATCCTGGTGGGGCTGACGG
TCATCGGGGCCTTCCTCAACCTGGTCGTCCTCAGGTTCTTGACCATGAACAGTGAGGATGAGCGGCGGGA
TGCTGAAGAGAGGGCATCCCTCGCCGGAAACCGCAACAGCATGGTCATTCACATCCCTGAGGAGCCGCGG
CCCAGCCGGCCCAGGTACAAGGCGGACGTCCCGGACCTGCAGTCTGTGTGCTCCTGCACCTGCTACCGCT
CGCAGGACTATGGCGGCCGCTCGGTGGCACCGCAGAACTCCTTCAGCGCCAAGCTTGCCCCCCACTACTT
CCACTCCATCTCTTACAAGATCGAGGAGATCTCACCAAGCACATTAAAAAACAGCCTCTTCCCATCGCCT
ATTAGCTCCATCTCTCCTGGGTTACACAGCTTTACCGACCACCAGAGGCTGATGAAACGCCGGAAGTCCG
TT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211314 protein sequence
Red=Cloning site Green=Tags(s)

MKRQNVRTLSLIVCTFTYLLVGAAVFDALESDHEMREEEKLKAEEIRIKGKYNISSEDYRQLELVILQSE
PHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLL
KRIKKCCGMRNTDVSMENMVTVGFFSCMGTLCIGAAAFSQCEEWSFFHAYYYCFITLTTIGFGDYVALQT
KGALQKKPLYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASLAGNRNSMVIHIPEEPR
PSRPRYKADVPDLQSVCSCTCYRSQDYGGRSVAPQNSFSAKLAPHYFHSISYKIEEISPSTLKNSLFPSP
ISSISPGLHSFTDHQRLMKRRKSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016601
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016601.2, NP_057685.1
RefSeq Size 3300 bp
RefSeq ORF 1124 bp
Locus ID 51305
Cytogenetics 8q24.3
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 42.3 kDa
Summary This gene encodes a protein that contains multiple transmembrane regions and two pore-forming P domains and functions as a pH-dependent potassium channel. Amplification and overexpression of this gene have been observed in several types of human carcinomas. This gene is imprinted in the brain, with preferential expression from the maternal allele. A mutation in this gene was associated with Birk-Barel dysmorphism syndrome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2017
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Documents
Resources
"NM_016601" in other vectors (4)
SKU Description Size Price
RC211314L3 Lenti ORF clone of Human potassium channel, subfamily K, member 9 (KCNK9), Myc-DDK-tagged 10 ug
$757.00
RC211314L4 Lenti ORF clone of Human potassium channel, subfamily K, member 9 (KCNK9), mGFP tagged 10 ug
$757.00
RG211314 KCNK9 (tGFP-tagged) - Human potassium channel, subfamily K, member 9 (KCNK9) 10 ug
$489.00 $657.00
SC304437 KCNK9 (untagged)-Human potassium channel, subfamily K, member 9 (KCNK9) 10 ug
$457.00

Plasmid Map for RC211314

Download sequence for

Cloning scheme for RC211314

Circular map for RC211314

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