RAB36 (NM_004914) Human Tagged ORF Clone

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SKU
RC211227
RAB36 (Myc-DDK-tagged)-Human RAB36, member RAS oncogene family (RAB36)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RAB36
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211227 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATCGCCGGTGCAAGCTGGATGCTTGGACGCGCCGCTGCCAGTCCAACGCAGACCCCGCCCACGA
CGTCGACGATTCGTGTAGCCCGCAGGTCCCGCGTGGCTCTCGTTGCCATGGTGATCGCCGCCGCTGGCTC
AGGCGGACCAGGCCGCGCGGAGCCCCAGCTTTCACAGCCATCGCTGGACTGTGGAAGAATGAGGTCCTCC
CTGACACCTTTGGGGCCCCCTGTGAGCCGCGACCGTGTCATCGCCAGCTTCCCTAAGTGGTACACGCCGG
AAGCCTGTTTGCAGCTCAGGGAGCACTTCCACGGGCAGGTCAGCGCTGCCTGCCAACGCAGGAACACGGG
GACTGTCGGGCTCAAACTCTCCAAGGTGGTGGTGGTTGGCGATCTCTACGTGGGGAAGACCAGCCTCATC
CACAGGTTTTGCAAGAATGTTTTTGATCGAGACTACAAGGCCACCATTGGGGTGGACTTTGAAATTGAGC
GCTTTGAGATTGCTGGGATTCCCTATAGCCTCCAGATCTGGGACACAGCTGGGCAGGAGAAGTTCAAGTG
CATCGCATCTGCCTACTACCGGGGTGCCCAGGTGATCATCACGGCCTTTGACCTCACTGACGTGCAGACC
CTGGAGCATACCAGGCAGTGGTTGGAGGATGCTCTGAGGGAGAACGAGGCAGGCTCCTGCTTCATCTTCC
TCGTGGGAACCAAGAAGGACCTTCTGTCAGGGGCCGCATGTGAGCAGGCCGAAGCAGACGCTGTGCACCT
GGCCAGGGAGATGCAGGCCGAGTACTGGTCAGTGTCGGCCAAGACTGGCGAGAACGTGAAGGCATTCTTC
AGCCGCGTAGCCGCCCTGGCATTCGAGCAGTCGGTGCTGCAGGACCTGGAGAGGCAGAGCAGTGCCCGGC
TCCAGGTCGGCAATGGAGACCTAATCCAAATGGAAGGGAGTCCGCCCGAGACCCAGGAGAGCAAGAGGCC
CTCCAGCCTGGGCTGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211227 protein sequence
Red=Cloning site Green=Tags(s)

MVIAGASWMLGRAAASPTQTPPTTSTIRVARRSRVALVAMVIAAAGSGGPGRAEPQLSQPSLDCGRMRSS
LTPLGPPVSRDRVIASFPKWYTPEACLQLREHFHGQVSAACQRRNTGTVGLKLSKVVVVGDLYVGKTSLI
HRFCKNVFDRDYKATIGVDFEIERFEIAGIPYSLQIWDTAGQEKFKCIASAYYRGAQVIITAFDLTDVQT
LEHTRQWLEDALRENEAGSCFIFLVGTKKDLLSGAACEQAEADAVHLAREMQAEYWSVSAKTGENVKAFF
SRVAALAFEQSVLQDLERQSSARLQVGNGDLIQMEGSPPETQESKRPSSLGCC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004914
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004914.4
RefSeq Size 3822 bp
RefSeq ORF 804 bp
Locus ID 9609
UniProt ID O95755
Cytogenetics 22q11.23
Protein Families Druggable Genome
MW 36.3 kDa
Summary Protein transport. Probably involved in vesicular traffic (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_004914" in other vectors (4)
SKU Description Size Price
RC211227L3 Lenti ORF clone of Human RAB36, member RAS oncogene family (RAB36), Myc-DDK-tagged 10 ug
$600.00
RC211227L4 Lenti ORF clone of Human RAB36, member RAS oncogene family (RAB36), mGFP tagged 10 ug
$600.00
RG211227 RAB36 (tGFP-tagged) - Human RAB36, member RAS oncogene family (RAB36) 10 ug
$489.00 $500.00
SC303546 RAB36 (untagged)-Human RAB36, member RAS oncogene family (RAB36) 10 ug
$457.00

Plasmid Map for RC211227

Download sequence for

Cloning scheme for RC211227

Circular map for RC211227

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