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GLIS1 (NM_147193) Human Tagged ORF Clone

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SKU
RC211202
GLIS1 (Myc-DDK-tagged)-Human GLIS family zinc finger 1 (GLIS1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $577.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GLIS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211202 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCCCGCACATCCCTGTCTGCCCACTGTCGGGGCCCGCTGGCCACTGGCCTGCACCCAGACC
TGGACCTCCCGGGCCGAAGCCTCGCCACCCCTGCGCCTTCCTGCTACCTTCTGGGCAGCGAACCCAGCTC
TGGCCTGGGCCTCCAGCCCGAGACCCACCTCCCCGAGGGCAGCCTGAAGCGGTGCTGCGTCTTGGGCCTA
CCCCCCACCTCCCCAGCCTCCTCCTCACCCTGTGCCTCCTCCGACGTCACCTCCATCATCCGCTCCTCCC
AGACGTCTCTGGTCACCTGTGTAAATGGACTCCGGAGCCCCCCTCTGACGGGAGATCTGGGGGGCCCTTC
CAAGCGGGCCCGGCCTGGCCCTGCATCGACGGACAGCCATGAGGGCAGCTTGCAACTTGAAGCCTGCCGG
AAGGCGAGCTTCCTGAAGCAGGAACCCGCGGATGAGTTTTCAGAGCTCTTTGGGCCTCACCAGCAGGGCC
TGCCGCCCCCCTATCCCCTGTCTCAGTTGCCGCCTGGCCCAAGCCTTGGAGGCCTGGGGCTGGGCCTGGC
AGGCAGGGTGGTGGCCGGGCGGCAGGCGTGCCGCTGGGTGGACTGCTGTGCAGCCTATGAGCAGCAGGAG
GAGCTGGTGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAGGACTTCACCTGCTTCTGGG
CTGGCTGCGTGCGCCGCTACAAGCCCTTCAACGCCCGCTACAAGCTGCTCATCCACATGCGAGTGCACTC
GGGCGAGAAGCCCAACAAGTGCATGTTTGAAGGCTGCAGCAAGGCCTTCTCACGGCTGGAGAACCTCAAG
ATCCACCTGAGGAGCCACACGGGCGAGAAGCCGTACCTGTGCCAGCACCCGGGTTGCCAGAAGGCCTTCA
GCAACTCCAGCGACCGCGCCAAGCACCAGCGCACCCACCTAGACACGAAGCCGTACGCCTGTCAGATCCC
TGGCTGCTCCAAGCGCTACACAGACCCCAGCTCCCTCCGCAAGCACGTCAAGGCCCATTCAGCCAAAGAG
CAGCAGGTGCGTAAGAAGCTGCATGCGGGCCCTGACACCGAGGCCGACGTCCTGACCGAGTGTCTGGTCC
TGCAGCAGCTCCACACGTCCACACAGCTGGCTGCCAGCGACGGCAAGGGTGGCTGTGGCCTGGGCCAGGA
GCTGCTCCCAGGTGTGTATCCTGGCTCCATCACCCCCCATAACGGACTTGCATCGGGCCTCCTGCCCCCA
GCGCACGACGTACCTTCCAGGCACCACCCGCTGGATGCCACCACCAGTTCCCACCACCATCTGTCCCCTC
TGCCCATGGCTGAGAGCACCCGGGATGGGTTGGGGCCCGGCCTCCTCTCACCAATAGTCAGCCCCCTGAA
GGGGCTGGGGCCACCGCCGCTGCCCCCATCCTCTCAGAGCCATTCTCCGGGGGGCCAGCCCTTCCCCACA
CTCCCCAGCAAGCCGTCCTACCCACCCTTCCAGAGCCCTCCACCCCCGCCTCTGCCCAGCCCACAAGGTT
ACCAGGGCAGTTTCCACTCCATCCAGAGTTGCTTCCCCTATGGCGACTGCTACCGGATGGCTGAACCAGC
AGCCGGTGGGGACGGACTGGTCGGGGAGACCCACGGTTTCAACCCCCTGCGGCCCAATGGCTACCACAGC
CTCAGCACGCCCTTGCCTGCCACAGGCTATGAGGCCCTGGCTGAGGCCTCATGCCCCACAGCGCTGCCAC
AGCAGCCATCTGAAGATGTGGTGTCCAGCGGCCCCGAGGACTGTGGCTTCTTCCCCAATGGAGCCTTTGA
CCACTGCCTGGGCCACATCCCCTCCATCTACACAGACACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211202 protein sequence
Red=Cloning site Green=Tags(s)

MAEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGL
PPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACR
KASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQE
ELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLK
IHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKE
QQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPP
AHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPT
LPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHS
LSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_147193
ORF Size 1860 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_147193.2
RefSeq Size 2816 bp
RefSeq ORF 1863 bp
Locus ID 148979
UniProt ID Q8NBF1
Cytogenetics 1p32.3
MW 66 kDa
Summary GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 PubMed 12042312).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_147193" in other vectors (6)
SKU Description Size Price
RC211202L1 Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), Myc-DDK-tagged 10 ug
$877.00
RC211202L2 Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), mGFP tagged 10 ug
$877.00
RC211202L3 Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), Myc-DDK-tagged 10 ug
$877.00
RC211202L4 Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), mGFP tagged 10 ug
$877.00
RG211202 GLIS1 (tGFP-tagged) - Human GLIS family zinc finger 1 (GLIS1) 10 ug
$489.00 $777.00
SC306307 GLIS1 (untagged)-Human GLIS family zinc finger 1 (GLIS1) 10 ug
$578.00

Plasmid Map for RC211202

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211202
Circular map for RC211202

file-alt Citations

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